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Fig. 5 | BMC Plant Biology

Fig. 5

From: The DAG1 transcription factor negatively regulates the seed-to-seedling transition in Arabidopsis acting on ABA and GA levels

Fig. 5

DAG1 binds the promoter of CYP707A2. Top: graphic representation of the CYP707A2 promoter. Underlying thick lines marked by letters (A, B, C) are referred to different promoter fragments used for qPCR, containing 0, 2 and 10 Dof sites respectively. Bottom, chromatin from dag1DAG1-HA (right) seeds and from dag1 (left) seeds, as a negative control, was immunoprecipitated with anti-HA antibodies, and the amount of DNA was measured by qPCR. The values of fold enrichment were normalized to internal controls (relative to input and to PP2A), and are the average of two biological replicates presented with SD values. Significant fold enrichments were analyzed by t-test (*P ≤ 0,05)

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