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Fig. 6 | BMC Plant Biology

Fig. 6

From: The DAG1 transcription factor negatively regulates the seed-to-seedling transition in Arabidopsis acting on ABA and GA levels

Fig. 6

GA induces DAG1 expression. a Relative expression level of DAG1 in 24 h-imbibed wild type (WT) seeds, in the presence of water (H2O), GA4+7 (100 μM), Paclobutrazol (PAC) (100 μM), or ABA (3 μM). The values of relative expression levels are the mean of three biological replicates, presented with SD values. Expression levels were normalized with that of the UBQ10 (At4g05320) gene. b and c Chromatin from seeds imbibed 24 h with H2O (white bars) or GA4+7 (100 μM) (black bars), was immunoprecipitated with specific antibodies against the H3K27me3 (b), or the H3K4me3 (c) epigenetic marks. The amount of DNA was measured by qPCR. The regions of the DAG1 locus used for qPCR are as in Fig. 1. The values of fold enrichment were normalized to internal controls (relative to input and to PP2A), and are the average of two biological replicates presented with SD values. Significant differences were analyzed by t-test (*P ≤ 0,05)

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