Fig. 7
The DAG1 protein is stabilized by GA. a Protein level of DAG1-HA in dag1DAG1:HA seeds imbibed for 24 or 48 h in the presence of water (H2O), GA4+7, paclobutrazol (PAC) or ABA. b Protein level of DAG1-HA in 48 h-imbibed seeds (0), then treated for 4 or 8 h with cycloheximide (CHX), or with CHX and GA. Western blot (top) and densitometric analysis (bottom). c Protein level of DAG1-HA in 5-day-old dag1DAG1:HA seedlings treated with MG132 or DMSO as control. TUB or total protein content were used as loading control. Western blot (top) and densitometric analysis (bottom). GA4+7 (100 μM), PAC (100 μM), ABA (3 μM), CHX (50 μM), MG132 (50 μM). The protein levels are the mean of three biological replicates, presented with SD values. Significant differences were analyzed by t-test (*P ≤ 0,05)