Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo

Fig. 3

WUS is an MPK3 substrate in vivo. a-c Electropherograms of various WUS:myc fusion proteins and their isoform distributions in cIEF-immunoassay. Expressed proteins and treatments are indicated for each sample. a Effect of activated MPK3 on C-terminal myc-tagged WUS isoform distributions in cIEF-immunoassay. Top: control (single WUS:myc construct transformation), middle: WUS:myc co-expressed with MPK3, bottom: WUS:myc co-expressed with flg22-activated MPK3. Asterisks indicate acidic isoforms specifically accumulating in the presence of activated MPK3. b Differential charge compositions of point mutant WUS variants are detected as changes in protein pI values. WUSAA: non-phosphorylatable mutant, WUS-DD: phosphomimetic mutant, WUS-Δdock: MAPK docking D-site disabled mutant. c Alanine substitutions at the MAPK phosphorylation sites T108, S112 impair WUS phosphorylation by MPK3. d Conventional SDS-PAGE immunoblot of transiently expressed WUS variants

Back to article page