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Table 1 Peak data for ACS6 C-terminal domain phosphorylation

From: Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo

pI

Area %: GFP:ACS6-C +

pI

Area %: GFP:ACS6-C-AAA +

-

MPK6

MPK6 + flg

-

MPK6

MPK6 + flg

4.58

2.7

5.46

3.09

4.59

5.13

6.05

3.09

4.75

6.04

7.23

7.19

4.75

7

7.89

7.21

4.82

9.64

7.33

10.02

4.82

11.02

9.04

9.1

4.89

13.35

9.43

21.45

4.88

16.48

18.74

13.1

5.01

1.54

17.04

20.56

5.03

4.85

5.25

4.48

5.11

0

0

11.44

5.15

8.04

7.93

10.2

5.15

7.09

6.32

4.29

    

5.28

23.14

20.46

14.36

5.27

25.15

23.1

27.82

5.39

0

4.73

0

5.39

0

6.4

0

5.44

23.08

14.98

1.58

5.45

5.8

0

8.66

5.55

0

0

3.67

5.55

11.24

10.93

10.48

5.68

11.53

7.02

0

    

5.74

0

0

2.34

5.73

5.28

4.67

5.86

  1. Data correspond to the electropherograms shown in Fig. 2b, c