Fig. 1

Structural feature of ONAC095, cis-elements in promoter of ONAC095 and stress-responsive expression of ONAC095. a Alignment of ONAC095 with rice ONAC022 and Arabidopsis ANAC036. The conserved NAC domain is boxed in red and the five highly conserved subdomains A to E are indicated by black arrowed lines. C1 and C2 domains are boxed with green and blue lines, respectively. Putative phosphorylation sites are indicated by arrows. S, serine; T, threonine; Y, tyrosine. b Distribution of major stress-related cis-elements in the promoter (1.5 Kb upstream of the start codon) of the ONAC095 gene. c Stress-responsive expression of ONAC095 by drought, salt, cold and heat stresses as well as by exogenous ABA. Drought stress was applied to two-week-old seedlings by transferring the seedlings to three layers filter papers for fast dehydration. Salt stress was applied to the seedlings by rooting the seedlings in 150 mM NaCl solution. Cold and heat stresses were applied by placing the seedlings in growth chambers with temperatures set at 4 °C or 42 °C, respectively. For ABA treatment, the seedlings were sprayed with 100 μM ABA or similar volume of solution as controls. Relative expression levels of ONAC095 were normalized by the transcript level of the Actin gene and the expression level was set as 1 at 0 hr after treatment. Data presented in c are the means ± SD from three independent experiments and columns with an asterisk indicate significant difference at p < 0.05 level between the treatments and normal controls