Fig. 2

Transactivation activity and nuclear localization of ONAC095. a–d Transactivation activity and mapping of the specific sequence responsible for transactivation activity in ONAC095. a and c Diagrams showing different truncated constructs and mutated amino acid residues in C2 domain, respectively. b and d Transcription activity assay for truncated and mutated constructs of ONAC095 in yeast. Yeasts harboring different truncated and mutated constructs and empty vector were grown on the SD/Trp⁻ plates or SD/Trp⁻ His⁻ with 4 mM 3-AT for 3 days at 30 °C. Transactivation activity was examined by the growth ability and production of blue pigment after addition of X-α-gal in the SD/Trp⁻ His⁻ plates for 1 day. e ONAC095 is localized in nucleus. Agrobacteria harboring pFGC-ONAC095 or pFGC-eGFP were infiltrated into leaves of N. benthamiana plants expressing a red nucleus marker protein RFP-H2B and leaf samples were collected at 24 hr after agroinfiltration. Microscopic examination was performed under a confocal laser scanning microscope in dark field for green fluorescence (left), red fluorescence (middle left), white field for cell morphology (middle right) and in combination (right), respectively