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Fig. 3 | BMC Plant Biology

Fig. 3

From: Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance

Fig. 3

Characterization of ONAC095-OE and ONAC095-SRDX transgenic rice lines and their growth phenotypes. a Schematic diagrams showing the overexpression ONAC095-OE and the dominant chimeric repressor-mediated suppression ONAC095-SRDX constructs used for transformation. HptII, hygromycin phosphotransferase II; LB, left border; RB, right border; Ubi, maize ubiquitin promoter; 35S, CaMV 35S promoter; GUS, β-glucuronidase. b Confirmation of single-copy transgenic lines by Southern blot analysis. Fifty micrograms of genomic DNA were digested with EcoRI and probed with a DIG-labeled fragment of the HptII gene. c Transcript levels of ONAC095 and ONAC095-SRDX in ONAC095-OE and ONAC095-SRDX transgenic lines. Leaf samples from two-week-old seedlings were used for analysis of the transcript levels by qRT-PCR. d Transcript levels of ONAC022 in ONAC095-OE and ONAC095-SRDX transgenic lines. Leaf samples from two-week-old seedlings were used for analysis of the transcript levels by qRT-PCR. e Growth phenotype of two-month-old ONAC095-OE and ONAC095-SRDX plants grown under normal watered condition in greenhouse. f and g Plant height and root length of two-month-old ONAC095-OE and ONAC095-SRDX plants grown under normal watered condition in greenhouse. h Weights of 1000-grain from ONAC095-OE and ONAC095-SRDX plants grown under normal watered condition in greenhouse. Data presented (c, d, f, g) and (h) are the mean ± SD from three independent experiments and columns with an asterisk indicate significant difference at p ≤ 0.05 level between WT and OE/SRDX lines. WT, wild type; OE6, ONAC095-OE6; OE12, ONAC095-OE12; S2, ONAC095-SRDX2; S3, ONAC095-SRDX3

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