Fig. 2

The ssi2-2 mutant harbors a new allele of SSI2. a Physical mapping of ssi2-2. Rough mapping showed that the ssi2-2 gene is located between markers NGA168 and CER461445 on chromosome 2 of Arabidopsis. Fine mapping revealed the gene between the primers of CAPS5 and CH2.1814, a range of 83.8 kb. The numbers below the molecular markers indicate the recombinant events detected between the ssi2-2 locus and the marker. b Structure of the ssi2-2 gene, AT2G43710. AT2G43710 encodes stearoyl desaturase and has three exons and two introns; sequence analysis revealed two point mutations in the third exon of the gene, namely G1952A and G2118A, resulting in changes in amino acids 257 and 312