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Fig. 3 | BMC Plant Biology

Fig. 3

From: Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1

Fig. 3

Modulation of plant growth in vivo. a Wild-type control plants grown for 2 weeks on MS plates containing the solvent DMSO and no bulky kinase inhibitor. Scale bar: 4 mm. b Wild-type plants grown for 2 weeks on MS plates supplemented with 100 μM 1-NA-PP1 die. Scale bar: 4 mm. c Wild-type plants grown for 2 weeks on MS plates supplemented with 100 μM 1-NM-PP1 are smaller than wild-type plants grown without the inhibitor but survive. Scale bar: 4 mm. d cdka;1 −/− PRO CDKA;1 :CDKA;1 F80G (cdka;1-as) grown for 2 weeks on MS plates containing the solvent DMSO and no bulky kinase inhibitor are slight reduced in their size in comparison with the wild-type control plants, see also Fig. 2a, d. Scale bar: 4 mm. e cdka;1-as grown for 2 weeks on MS plates supplemented with 100 μM 1-NM-PP1 is severely compromised with arrested root development. Scale bar: 4 mm. f A homozygous cdka;1 −/− seedling grown on a MS plate for 2 weeks after germination shows the typical phenotype of loss of CDKA;1 function with halted root development and only a few and tiny leaves being formed. Scale bar: 1 mm. g Root length measurement of the seedlings of wild type (Col-0) and cdka;1-as (as) grown on MS plates supplemented with 10 μM 1-NM-PP1 for 7 days after germination. Error bars represent the SE. A statistical significant change between the mock and 1-NM-PP1 treatment is marked by an asterisk above the bar (t-test, P = 0.0002 <0.05)

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