Fig. 1
Design of TALENs targeting FAD3 genes within Glycine max. a Illustration of the fatty acid pathway. Relative percent composition of individual fatty acids in the oil from WT and fad2-1 knockout plants is shown on the right. b Schematic of the FAD3A genomic sequence. Triangles, approximate TALEN binding sites; black boxes, exons; gray boxes, 5’ and 3’ untranslated regions. c Nucleotide sequences of the predicted TALEN target sites within the FAD3A, FAD3B, and FAD3A genes. Bold and underlined nucleotides indicate TALEN binding sequence. Lower case nucleotides indicate positions of SNPs. d Illustration of a TALEN monomer expression vector. PNOS, nopaline synthase promoter; SV40 NLS, simian virus 40 large T-antigen nuclear localization signal; TNOS, nopaline synthase terminator; AmpR, ampicillin resistance gene