Fig. 2From: Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil FAD3 TALEN activity in soybean protoplasts. a TALEN pairs were assessed for their activity ~48 h after transformation in soybean protoplast. The frequency of mutagenesis represents the total number of sequence reads with insertions or deletions divided by the total number of sequence reads. The resulting number was then divided by the transformation frequency (90 %) which was determined using a YFP control plasmid. b TALEN activity relative to the number of SNPs present within the predicted TALEN binding sitesBack to article page