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Table 2 Genotype of T1 plants from candidate T0 events harboring mutations within FAD3A

From: Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil

Parent line (T0)

T1 plant number

FAD3A genotype

Presence of transgene

Gm183-4

1

−7 bp/WT

Undetected

2

−7 bp/-7 bp

+

3

−7 bp/-7 bp

+

4

−7 bp/WT

+

Gm183-5

2

−43 bp/WT

+

3

WT/WT

Undetected

4

−43 bp/-43 bp

+

5

−43 bp/-43 bp

Undetected

7

−43 bp/-43 bp

+

8

−4 bp/-4 bp

+

9

−43 bp/-43 bp

+

Gm183-6

1

−4 bp/-4 bp

+

2

WT/WT

+

3

WT/WT

+

4

−4 bp/WT

+

5

−4 bp/-4 bp

+

6

WT/WT

Undetected

7

−4 bp/WT

+

8

WT/WT

Undetected

9

WT/WT

+

10

WT/WT

+

11

−4 bp/WT

+

12

−4 bp/WT

+

13

WT/WT

+

14

−4 bp/WT

+