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Fig. 2 | BMC Plant Biology

Fig. 2

From: Major Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves

Fig. 2

Induced PLCP and reduced VPE activities individually darkened leaves. Increased DCG-04 (a) and MV151 (b) labeling of PLCPs during senescence. Leaf extracts of equal fresh weights of individually darkened leaves were labeled for 5 hours with 2 μM DCG-04 at pH 6.5 or 2 μM MV151 at pH 4.5 and biotinylated proteins were detected using streptavidin-HRP (a) or fluorescent proteins were detected by scanning (b), respectively. *, endogenously biotinylated protein. c, d Accumulation of RD21A (c) and AALP (d) proteins in individually darkened leaves. Protein extracts of equal fresh weights of individually darkened leaves were separated and detected from protein blots using RD21A and AALP –specific antibodies, respectively. e Reduced AMS101 labeling of VPEs in individually darkened leaves. Leaf extracts of equal fresh weights of individually darkened leaves were labeled for 2 hours with 2 μM AMS101 at pH5.5 and analysed by fluorescence scanning. Coomassie stains of the membranes (a-d) or protein gel (e) is used as a control to show degradation of the large subunit of Rubisco (RBCL). The dotted line (a, b) indicates a removed lane from a western blot

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