Fig. 4

Major PLCP activities are depleted in senescent leaves of rd21A-1/aalp-1 double mutant plants without affecting Rubisco levels. a PLCP activity profiles of control (day 0) and senescent leaf (day 7) of rd21A-1, aalp-1, sag12-1 and ctb3-1 mutants and wild-type plants. b PLCP activity profiles of individually darkened leaves at 0, 3, 5 and 7 days of the rd21A-1/aalp-1 double mutant in comparison to wild-type (Col0) plants. Leaf extracts of equal fresh weights of individually darkened leaves were labeled for 5 hours with 0.2 μM DCG-04 at pH 6.5 and biotinylated proteins were detected from protein blots using streptavidin-HRP