Fig. 6
Detection of reactive oxygen species (ROS) in Arabidopsis. a H2O2 accumulation was detected with DAB in 3-week-old rosette leaves after heat treatment at 45 °C for 1 h. O2 − accumulation was detected with NBT in 3-week-old rosette leaves after heat treatment at 45 °C for 1 h. b H2O2 content in 10-day-old seedlings after heat treatment at 45 °C for 1 h. c The activity of the antioxidant enzyme CAT in 10-day-old seedlings after heat treatment at 45 °C for 1 h. d The activity of the antioxidant enzyme GR in 10-day-old seedlings after heat treatment at 45 °C for 1 h. The data represent mean values ± SD of three independent experiments. (* indicates significance at P < 0.05; ** indicates significance at P < 0.01)