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Fig. 2 | BMC Plant Biology

Fig. 2

From: Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice

Fig. 2

Plasma membrane (PM) purity verification and PM-related proteins evaluation. a Western blot examination of plasma membrane enrichment. Proteins from entire cell (EC) lysates, microsomal vesicles (MSV), PM (plasma membrane vesicles) and carbonate-washed PM (CPM) were separated by 10% SDS-PAGE, transferred to PVDF membranes and detected with antibodies for PMA2, a PM marker; COXII, a mitochondrial marker; V-ATPase, a vacuole marker; S14-1, a ribosome marker; or H1, a nucleus marker. For detection of PMA2, COXII and V-ATPase, 5 μg protein was loaded per lane; for detection of S14-1 and H1, 10 μg protein was loaded per lane. b Summary of proteins with transmembrane domain (TMD) predicted with use of HMMTOP 2.0. c Venn diagram depicting the distribution of proteins with different lipid modifications. GPI, glycosylphosphatidylinositol anchor; Pre, prenylation site; Myr, myristoylation site; Pal, palmitoylation site. d Proteins predicted to have a TMD or post-translational modification (PTM) or both. e Protein subcellular locations annotated by Gene Ontology or WoLF PSORT showed that 1,121 of the 1,797 proteins with a TMD and/or PTM had PM location information

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BMC Plant Biology

ISSN: 1471-2229

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