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Fig. 4 | BMC Plant Biology

Fig. 4

From: Identification of brassinosteroid genes in Brachypodium distachyon

Fig. 4

Feedback regulation of Brachypodium BR biosynthetic genes after Pcz treatments. a Semi-quantitative RT-PCR of putative BR biosynthetic genes BdDWF4, BdBR6OX2, and BdCPD and the GA biosynthetic gene BdGA20ox after treatment with the indicated concentrations of Pcz and a mock control. BdACT7 is used as an internal loading control. b-d Quantitative real-time RT-PCR analysis evaluating the expression of the same genes under just mock or 50 μM Pcz

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