- Research article
- Open Access
Chloroplast genome resources and molecular markers differentiate rubber dandelion species from weedy relatives
BMC Plant Biology volume 17, Article number: 34 (2017)
Rubber dandelion (Taraxacum kok-saghyz, TK) is being developed as a domestic source of natural rubber to meet increasing global demand. However, the domestication of TK is complicated by its colocation with two weedy dandelion species, Taraxacum brevicorniculatum (TB) and the common dandelion (Taraxacum officinale, TO). TB is often present as a seed contaminant within TK accessions, while TO is a pandemic weed, which may have the potential to hybridize with TK. To discriminate these species at the molecular level, and facilitate gene flow studies between the potential rubber crop, TK, and its weedy relatives, we generated genomic and marker resources for these three dandelion species.
Complete chloroplast genome sequences of TK (151,338 bp), TO (151,299 bp), and TB (151,282 bp) were obtained using the Illumina GAII and MiSeq platforms. Chloroplast sequences were analyzed and annotated for all the three species. Phylogenetic analysis within Asteraceae showed that TK has a closer genetic distance to TB than to TO and Taraxacum species were most closely related to lettuce (Lactuca sativa). By sequencing multiple genotypes for each species and testing variants using gel-based methods, four chloroplast Single Nucleotide Polymorphism (SNP) variants were found to be fixed between TK and TO in large populations, and between TB and TO. Additionally, Expressed Sequence Tag (EST) resources developed for TO and TK permitted the identification of five nuclear species-specific SNP markers.
The availability of chloroplast genomes of these three dandelion species, as well as chloroplast and nuclear molecular markers, will provide a powerful genetic resource for germplasm differentiation and purification, and the study of potential gene flow among Taraxacum species.
Rubber dandelion (Taraxacum kok-saghyz Rodin, TK) is being developed as an alternative natural rubber source in response to increasing global demand and instability of current sources. Natural rubber production is fragile due to its reliance on a single source, the Brazilian or Para rubber tree (Hevea brasiliensis Muell. Arg.), which is cultivated as clones mostly in Southeast Asia . This production could be easily disrupted by the introduction of South American Leaf Blight, a fatal fungal disease caused by Microcyclus ulei , which is currently controlled by quarantine measures. Moreover, Hevea rubber production is also threatened by high labor costs, due to the necessity of tapping latex from the trees by hand, and land competition with palm plantations . To establish a more sustainable and mechanized natural rubber production system, TK has been explored in many temperate countries as a potential domestic rubber-producing crop .
TK, which originated in southeastern Kazakhstan as a wild plant , is a diploid (2x = 16) outcrossing, self-incompatible species. TK was cultivated extensively in the Union of Soviet Socialist Republics (USSR) and the US throughout the 1930s and during World War II to help alleviate wartime-induced natural rubber shortages . At that time, rubber yields for TK were reported between 150 and 500 kg ha−1 y−1 . Higher rubber production potential of TK has recently been demonstrated in studies where germplasm with a rubber content of 5–6% of root dry weight was grown in outdoor planting boxes to yield the equivalent of 1300 kg ha−1 in a 6-month period (Cornish, unpublished), which is comparable to the yield of rubber tree (500–3000 kg ha−1 y−1) . Reaching comparable yields in large scale field production is a challenging endeavor, but coupled with germplasm with much higher rubber concentrations (up to 30%), commercially viable yields appear achievable . Moreover, its wide environmental adaptation and fast generation time make TK one of the most promising alternate rubber producing plants. Rubber production from TK is expected to reduce the need to import rubber, mitigate production shortfalls, stabilize global rubber prices, as well as ensure rubber supplies should rubber tree production be threatened.
The domestication of TK is complicated by two additional dandelion species, Taraxacum brevicorniculatum Koroleva (TB) and Taraxacum officinale F.H. Wigg. (TO, common dandelion). TK, TB, and TO are sympatric species, and germplasm collections are often mixed . TB is a triploid (3x = 24), which exhibits obligate apomixis, where clonal seeds are produced without pollination. TB also produces natural rubber in its roots, albeit to a lesser extent than TK (approximately 2–3% of the dry weight in TB, compared to as high as 30% in TK) [5, 6]. However, TB is a more vigorous species than TK with a high accumulation of biomass similar to TO. Recent molecular biology studies have used TB to investigate functions of genes related to rubber biosynthesis [6–8]. TB and TK share the same geographical origin and have been co-introduced into North America and Europe, where TB is often an unintentional seed contaminant. Therefore, TB has often been misidentified as TK in many ex situ germplasm collections until TB and TK were discriminated using taxonomic and Amplified Fragment Length Polymorphism (AFLP) analyses .
TO, the ubiquitous weedy dandelion, is distributed worldwide and can be found in all states and provinces of the United States and Canada, respectively . TO has virtually no rubber production, although it does produces a milky latex, and is a vigorous, highly successful weed. TO is a perennial and is most successful as an agricultural weed in pastures and no-till systems. All TO reported in North America are obligate apomictic triploids (3x = 24) [10, 11]. However, sexual, diploid TO (2x = 16) has been identified in Europe .
TK domestication would involve large plantings and possibly the introduction of genetic modifications to improve agronomic performance and rubber yield. The potential for TK and TO to hybridize raises concerns about gene flow between species. There are two potential pathways of gene flow: pollen-mediated gene flow and seed-mediated gene flow . In pollen-mediated gene flow, transgenes contained in TK pollen could potentially be introduced into TO and produce hybrid progeny with novel traits. Alternatively, TK could potentially serve as the pollen acceptor and be fertilized by TO pollen to produce hybrid progeny with weedy traits. In the case of seed-mediated gene flow, progeny produced by TK could be from TK x TK crosses, interspecific hybridization, or through the “mentor effect”, where self-incompatibility is broken down by the introduction of polyploid pollen [14, 15]. Similarly, in the case of pollen mediated gene flow, apomictically produced TO seeds would inevitably be mixed with the seeds of potential hybrids. In order to understand the potential for gene flow between TK and TO, species-specific molecular markers are needed to differentiate interspecific hybrids from apomictically produced TO and self-pollinated TK.
Considering the differences in rubber content and reproduction system among the aforementioned three species, as well as the potential production of TK/TO hybrids, it is important to distinguish them in studies related to molecular genetics, genomics, plant breeding, and gene flow risk assessment. Since the fecundity of weedy dandelions (TB and TO) has been reported to be 40 times higher than TK, once seeds from weedy dandelion are introduced into TK fields, the contamination can be magnified significantly through a single generation . Information that can be used to resolve three dandelion species, as well as their potential hybrids, includes data on morphology and ploidy. However, morphological data may vary through developmental stage and is highly reliant on the experience of the observers. Ploidy detection using flow cytometry cannot be easily multiplexed and has a high cost of entry. Moreover, morphology and genome size of a potential hybrid may overlap with those of the three dandelion species. Therefore, it is necessary to develop molecular markers to provide an accurate and high throughput approach for species and hybrid differentiation.
One source of sequence diversity that can be used to differentiate species is the chloroplast genome. Due to the slower evolution of chloroplast genomes compared to nuclear genomes, chloroplast sequences have often been used for phylogenetic studies and species identification [16, 17]. Therefore, the development of chloroplast markers will provide an accurate molecular tool to differentiate Taraxacum species. Moreover, the genetic information in angiosperm chloroplasts is inherited maternally in most cases, making the chloroplast genome a good indicator of maternal ancestry . The maternal parent could be easily identified in putative hybrid progeny in the absence of parental information, regardless of how many generations have past.
In previous studies, barcoding regions generated from chloroplast sequences have been used for phylogenetic analysis and species differentiation [16, 19]. However, the application of barcoding regions involves re-sequencing those regions of the tested plant samples. Chloroplast Single Nucleotide Polymorphism (SNP) markers were developed in recent studies due to their greater abundance in the genome and better resolution of populations [20, 21]. Since SNP detection can be easily multiplexed and applied to large populations, species differentiation using SNP markers is more practical and conducive to larger experiments.
To identify hybrids, chloroplast markers alone are insufficient, as they are dominant and only indicate maternal ancestry; however, chloroplast markers may be complemented with markers from the nuclear genomes of these species. To date only limited genomic resources are available for TK and TO; 16,441 expressed sequence tags (ESTs) derived from TK root RNA can be found on the National Center for Biotechnology Information (NCBI) (Collins J, Whalen MC, Nural-Taban AH, Scott D, Hathwaik U, Lazo GR, Cox K, Durant K, Woolsey R, Schegg K, et al. Genomic and proteomic identification of candidates genes and proteins for rubber biosynthesis in Taraxacum kok-saghyz (Russian dandelion). 2009. Unpublished; Shintani D. Using EST from Taraxacum kok-saghyz root cDNA library to generate candidate rubber biosynthetic genes. 2005. Unpublished). More EST data obtained from whole plants (41,294 ESTs, 16,858 unigenes) are available for TO [22, 23]. No TB sequence data have been reported.
In this study, chloroplast genomes have been sequenced for TK, TB and TO and chloroplast markers have been developed and validated. At the same time, nuclear markers were developed using previously published ESTs. The genomic and marker resources described in this paper will not only provide a molecular toolkit for germplasm identification and purification, but also allow accurate gene flow studies between TK and TO.
Chloroplast genome sequencing
To generate a complete TK chloroplast genome sequence, chloroplast DNA was extracted from a mixture of genetically distinct TK plants. To reduce polysaccharide content, which interferes with DNA extraction, young leaves were harvested from 1 to 2 month-old greenhouse grown TK plants subjected to a 2-day dark treatment before harvesting. About 20 g leaf tissue were ground in liquid nitrogen and suspended in 400 ml grinding buffer (0.35 M sorbitol, 50 mM HEPES/KOH, pH 7.5, 2 mM EDTA, 1 mM MgCl2, 1 mM MnCl2 and 4.4 mM sodium ascorbate (added just before use) (modified from [24, 25]). After filtering the tissue through four layers of miracloth, the filtrate was collected by centrifuging at 4500 × g for 20 min. The re-suspended pellets were placed on the top of a 30–50% sucrose gradient and centrifuged for 45 min at 10,000 × g, at 4 °C, in a swinging bucket rotor. The intact chloroplasts formed a layer between the 30 and 50% sucrose and were separated from the broken chloroplast remnants. Isolated chloroplasts were treated by DNase using Ambion® TURBO DNA-free™ Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) to degrade nuclear DNA. Chloroplast DNA was extracted using GenElute™ Plant Genomic DNA Miniprep kit (Sigma-Aldrich®, St. Louis, MO, USA) and enriched using the REPLI-g® Mini Kit (Qiagen, Inc., Hilden, Germany). DNA quality was initially checked and quantified using a NanoDrop® ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). Distinctive individual band patterns shown after DNA digestion by restriction enzyme EcoRI indicated the high percentage of chloroplast DNA. DNA was submitted to The Molecular and Cellular Imaging Center (MCIC) at the Ohio Agricultural Research and Development Center (OARDC) for additional quality control and sequencing using the Illumina GAII sequencing platform.
To generate TK chloroplast genomes from multiple genotypes as well as complete TO and TB chloroplast genomes, three species were sequenced by MiSeq. A total of 24 genotypes were selected for TK, including 19 USDA lines, three mixed genotypes from USDA lines and a single cytoplasmic male sterile line (Additional file 1). All the USDA lines used in this study were obtained from the USDA-ARS National Plant Germplasm System (NPGS). These samples were collected in southeast Kazakhstan in 2008, from an area delineated by 42.79949 N to 43.06724 N, and 79.17952E to 80.08643E . Detailed information of this collection can be obtained through the NPGS database, Germplasm Resources Information Network (GRIN) at http://www.ars-grin.gov/npgs/ . Additional plants were selected from individual crosses between plants of specific USDA Accessions. All of the genotypes we selected to represent TK were self-incompatible and outcrossing, without variance in genome size. Twenty-four TO genotypes from a global collection of TO seed, including seed collected from North America, Europe and China, were used for sequencing (Additional file 2). All TO seeds used in this study were donated by weed scientists and other collaborators voluntarily, and collected by Prof. John Cardina (Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA). No permissions were required to obtain these seeds. TO seeds were identified based on the plant morphology and reproductive system. A TB “Clone A” donated by Peter van Dijk (Keygene, Wageningen, Netherlands), which originally came from the Botanical garden, Marburg, Germany, as well as 11 genotypes descended from plants collected from Kazakhstan and distributed broadly by Dr. Anvar Buranov (Nova-BioRubber Green Technologies Inc., Canada) were used for TB chloroplast sequencing (Additional file 3) . All TO and TB plants used produced full seed set without pollination and exhibited apomixis after emasculation, with the exception of a single diploid, sexual TO accession, which was deliberately included. The total DNA from 60 leaf samples was extracted using a 2% cetyl trimethylammonium bromide (CTAB) DNA extraction protocol . DNA amount was normalized to 1 ng μL−1 and used for entire chloroplast genome amplification by Long Range Polymerase Chain Reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs Inc., Ipswich, MA, USA). Primers were designed on the conserved regions of the draft TK chloroplast sequence generated by the Illumina GAII data (Additional file 4). Amplified fragments were normalized within each species to have the same molarity and submitted for MiSeq sequencing. The 24 genotypes of TK, 24 genotypes of TO and 12 genotypes of TB were sequenced in a single MiSeq run. A library was made for each species, which was tagged using different barcoding sequences to separate short reads for each species. Individual accessions were not tagged separately.
Chloroplast genome assembly and annotation
Paired-end reads were generated for multiple genotypes of TK, TO, and TB by the Illumina GAII and MiSeq sequencing platforms. Quality control was conducted using the FASTX-Toolkit . For TK GAII data, the quality cutoff score was 40 (-q). A quality score of 20 was used for all Miseq data. By using the assembly program Velvet (version 1.2.10), with parameters, kmer = 35, -cov_cutoff = 20, a complete TK chloroplast genome sequence was generated from high quality GAII short reads . Three contigs sized at 18,568, 24,353 and 84,064 bp long were generated. The 18,568 and 84,064 bp contigs had coverages of 344 and 343, respectively, representing the single copy regions. The 24,353 bp contig had a higher coverage of 834, as there are two copies of this region in a chloroplast haplotype. No Ns were included in the contigs. TO and TB short reads were assembled using the same method mentioned above with the quality score of 20. Assembled contigs were further mapped to the TK chloroplast genome as a reference by BLASTn to generate the entire chloroplast genomes .
Complete chloroplast genomes of TK, TO, and TB were annotated using the Dual Organellar GenoMe Annotator (DOGMA) . Annotation errors were manually corrected. An annotation map was generated using OrganellarGenomeDRAW (OGDRAW) .
Phylogenetic analysis in the Asteraceae and comparative analysis within Taraxacum genus
Phylogenetic analysis was conducted using the Rubisco (Ribulose-1, 5-bisphosphate carboxylase/oxygenase) large subunit gene rbcL from TK, TO, TB and other 27 species in the Asteraceae with available chloroplast genome sequences (Additional file 5). Multiple sequence alignments were carried out using ClustalW, followed by phylogenetic tree generation using MEGA6 . The Maximum Likelihood method was used and the tree with the highest log likelihood was obtained .
To analyze the similarities and divergences of the TK, TO, and TB chloroplast genomes, complete chloroplast sequences of these three species were input into the mVISTA program, along with their annotation information [36, 37]. The Shuffle-LAGAN mode was chosen for comparative analysis . The TK chloroplast sequence was used as the reference genome.
Chloroplast species-specific marker discovery
To develop chloroplast species-specific markers between TK and TO, TO short reads were mapped to the TK chloroplast genome sequence using Bowtie 2 . Variants between TK and TO were detected by Freebayes using the default parameters . TK short reads were further mapped to the TK chloroplast genome to eliminate variants which were not fixed within TK. Variants between TK and TO, but fixed within each species, were considered candidate species-specific markers.
Nuclear species-specific marker discovery
To develop nuclear species-specific markers using available Expressed Sequence Tag (EST) resources, 41,294 ESTs of TO (GenBank accession numbers: DY802201-DY843494) and 16,441 ESTs of TK (GenBank accession numbers: GO660574-GO672283, DR398435-DR403165) were obtained from NCBI [22, 23] (Collins J, Whalen MC, Nural-Taban AH, Scott D, Hathwaik U, Lazo GR, Cox K, Durant K, Woolsey R, Schegg K, et al. Genomic and proteomic identification of candidates genes and proteins for rubber biosynthesis in Taraxacum kok-saghyz (Russian dandelion). 2009. Unpublished; Shintani D. Using EST from Taraxacum kok-saghyz root cDNA library to generate candidate rubber biosynthetic genes. 2005. Unpublished). Using the pipeline described by Kozik (2007) , ESTs were assembled into contigs and filtered. Interspecific variants were selected manually, by screening alignments flagged as containing interspecific variations.
Species-specific marker validation
Markers were validated through gel based assays in larger populations than those used for sequencing for each species. The number of genotypes used for TK, TO and TB were 102, 103 and 24, respectively (Additional files 1, 2 and 3). Primers were designed by Primer 3 [42, 43] to validate Cleaved Amplified Polymorphic Sequences (CAPS), which were identified by CAPS Designer  using the following PCR procedure: 5 min initial denaturation at 95 °C, followed by 35 cycles of 40s denaturation at 95 °C, 60s annealing at 54 °C or 56 °C, 60s elongation at 68 °C, as well as a final extension step at 68 °C for 5 min. Tetra-primer ARMS-PCR was also carried out to detect SNPs using the similar PCR procedure with a 58 °C annealing temperature . All the PCR reactions were conducted using reagents obtained from New England Biolabs (Inc., Ipswich, MA, USA) in a 10 μL reaction, following the manufacturer’s instructions.
Chloroplast genome generation, characterization and annotation
More than 25 million paired-end reads were generated by the Illumina GAII sequencing platform for TK, while more than 10, 12, and 6 million reads were generated by MiSeq sequencing for multiple genotypes of TK, TO, and TB, respectively. After de nova and reference guided assembly, the complete chloroplast genome sequences of TK, TO, and TB were obtained and submitted to NCBI database with GenBank accession numbers KX198560 (TK), KX198561 (TO), and KX198559 (TB). The genome sizes of TK, TO, and TB were found to be 151,338, 151,299, and 151,282 bp, respectively. The genome sizes of these three species are similar to those of other species in the Asteraceae, which range from 149,510 to 153,202 bp [46, 47]. The chloroplast genome can be divided into four regions, which are one Large Single Copy (LSC) region, one Small Single Copy (SSC) region, and two Inverted Repeat (IR) regions. The genome size, and regions, as well as the GC content of each species are listed in Table 1. As previously reported in other Asteraceae, the chloroplast genomes of TK, TO, and TB contain a 21 k large inversion (Inv 1) and a 2.1 k small inversion (Inv 2) in the LSC region (Fig. 1) [19, 48, 49]. Inv 1 begins between gene trnS-GCU and trnC-GCA, and ends between trnR-UCU and trnT-GGU. Inv2 occurred at one end of Inv1 and shares the same starting point as Inv1. Inv2 ends between trnY-GUA and ropB.
The annotated chloroplast genomes of these three species are represented in one circular map since their gene number, order and names are the same (Fig. 1). A total of 134 genes have been identified for each of the three species, including 82 protein-coding genes, 8 rRNA genes, 36 tRNA genes, as well as 8 pseudogenes and Open Reading Frames (ORFs). There are 61 protein-coding genes and 21 tRNA genes located in the LSC region, while 11 protein-coding genes and 1 tRNA gene are located in the SSC region. All the rRNA genes are located in the IR regions, along with 5 protein-coding genes, 7 tRNA genes and 4 pseudogenes and ORFs. Genes located in the IR regions are duplicated except rps19 and ycf1, which were only partially duplicated. One specific feature of note is that gene trnF-GAA has three copies in TK and TB, but only two copies in TO. The copy number variation of the trnF-GAA gene has been considered a specific characteristic of Taraxacum sp., which might be useful as a resource for evolutionary studies [18, 50].
Phylogenetic analysis in the Asteraceae
Sequence alignment showed that TK, TO, and TB chloroplast genomes are highly homologous with other members of the Asteraceae. A phylogenetic tree showing the genetic relationship of species in the Asteraceae was obtained (Fig. 2). The results were consistent with previous studies; species within the same subfamily and tribe were grouped together [47, 51]. Phylogenetic analysis showed that TK has a closer genetic distance to TB than to TO. Of the species analyzed, the Taraxacum species were most closely related to lettuce (Lactuca sativa) (Fig. 2).
Comparative analysis of chloroplast genomes in Taraxacum genus
The complete sequences of TK, TO, and TB were compared and revealed specific highly divergent regions (Fig. 3). Overall, the three species were highly similar, with shared sequence identities of 99.6% in pairwise comparisons. Two IR regions are highly conserved among the species. Non-coding regions, including intergenic regions and introns, were more divergent than protein coding regions. Pairwise comparison between species revealed the gene coding regions with most variations. The first 15 regions with the lowest sequence identity are listed in Table 2. Gene accD (acetyl-CoA carboxylase carboxyltransferase beta subunit) showed the most divergence among the three species. Gene sequence identities of the 15 regions ranged from 97.97 to 99.79%, 96.34 to 99.81% and 94.38 to 99.79% for TK and TO, TK and TB, TO and TB comparisons, respectively. Additionally, non-coding regions with high divergence include inter spaces between trnR-UCU and trnT-GGU, trnM-CAU and atpE, petA and psbJ, trnW-CCA and trnP-UGG, ndhI and ndhG, rpl32 and ndhF, as well as the intron region of ndhA.
Chloroplast species-specific marker development
Variant calling revealed 218 intraspecific variants within 24 genotypes of TK, including 172 SNPs (with an average coverage of 1720, ranging from 46 to 8796), while only 31 intraspecific variants within 24 genotypes of TO were detected, including 12 SNPs (with an average coverage of 7082, ranging from 961 to 24,907). After mapping TO short reads to the TK chloroplast, a total of 281 variants were identified, including 205 SNPs. The average coverage was 1838, and ranged from 152 to 11,954. Among these SNPs, 16 were fixed between TK and TO, with an average coverage of 1708, ranging from 152 to 4296. The location, nucleotide change, annotation and mutation type of these 16 SNPs are listed in Table 3. Although nine SNPs were located in protein coding regions, only two SNPs, which was within gene accD and ndhA (NADH dehydrogenase subunit 1), caused non-synonymous mutations. Four CAPS markers and one Tetra-primer ARMS-PCR marker were chosen as candidate species-specific markers. The primers and restriction enzymes used for marker detection are included in Table 4. These five markers were further validated in a large TO population, including 24 genotypes used for MiSeq and another 59 genotypes from a TO seed world collection. The markers were also validated in a large TK population, which included multiple genotypes from the USDA TK collection and TK populations from current OSU breeding programs (Additional files 1 and 2). Three CAPS markers and one Tetra-primer ARMS-PCR marker showed fixed band patterns within each species, but these patterns were consistently different between species, suggesting that these markers can be used as species-specific markers to differentiate TK from TO. Combining multiple markers is not required since each single marker is sufficient for species differentiation in our study. Among four CAPS markers tested here, one showed polymorphic band patterns in the TO population and could be used as an intraspecific marker. The band patterns for each marker in each species are listed in Table 4.
Nuclear species-specific marker development
A total of 6187 contigs were assembled from existing TO and TK EST resources, totaling 4.2 Megabases (MB), containing 16,900 redundantly detected variants. Only variants detected more than once were counted to reduce noise caused by sequencing errors. A total of 23 redundant, putatively species-specific SNPs were tested as CAPS in the TK and TO populations mentioned above. Of these, two (9%) did not exhibit diversity, 16 (69%) were polymorphic within either or both species and five (22%) were fixed between TK and TO (Table 5). Although some of the five fixed markers showed polymorphic patterns within species, they still differed between the two species, and so were considered species-specific markers. Species-specific markers fixed between TK and TO, as well as intraspecific markers fixed in parental populations, can be further used to validate potential TK x TO hybrid populations in gene flow studies.
In this study, we generated chloroplast genomes for three Taraxacum species using Illumina GAII and MiSeq platforms, which provide important resources for ecological, evolutionary, and genetic engineering studies. The TO chloroplast genome was compared to previously published data and showed highly similarity in genome size and structure, gene number, as well as GC content . Using chloroplast sequences obtained here, and online EST data, we were able to develop species-specific and intraspecific molecular markers, which are essential tools for germplasm purification and gene flow studies. The relationship between different types of molecular markers and species differentiation are summarized in Fig. 4. In our study, we developed new strategies to conduct chloroplast sequencing to facilitate species-specific marker discovery. By pooling multiple genotypes with normalized molarity into one library, and sequencing three libraries in one MiSeq run, a wide range of variation was detected at low cost. After SNP calling, we further validated the candidate markers using populations containing a wide germplasm collection. Not all the candidate markers revealed by sequencing were confirmed as species-specific markers, indicating that only using sequencing data from limited genotypes is not sufficient to develop reliable species-specific markers. Gel-based assays used in this study allowed us to develop species-specific markers which can be used through broad populations, which is especially critical for outcrossing species (e.g. TK) and species with widespread geographic distribution (e.g. TO).
It is expected that the most likely avenue of hybridization between TK and TO is the pollination of TK by TO, as the majority of TO are obligate apomicts [10, 11]. TK was introduced into the U.S. during the Emergency Rubber Project in 1942, where it was hastily cultivated at 152 locations in 40 states as a source of rubber . Although the project was abandoned in 1944 , the massive introduction of TK gave considerable opportunity for gene flow between TK and its weedy relative TO. Although no TK plants appear to have persisted, no gene flow risk assessment between these two species has been reported. Maternally inherited chloroplast species-specific markers provided in this study can be used to detect ancestral hybridization in the field between TK mothers and TO fathers, even when many decades of potential backcrosses may have masked the TK phenotype in such hybrids. The recent development of TK as an alternative rubber resource has prompted new germplasm introductions from Uzbekistan and Kazakhstan in 2006 and 2009, respectively. These markers can also be used to proactively detect recent hybridizations, which may now be occurring.
In this study, intraspecific chloroplast and nuclear markers have been discovered, which may have uses in population genetics to test correlations between genetic information carried by chloroplast and nuclear genomes and geographic or environmental data. Intraspecific markers can be used to characterize population structures, revealing information about local adaptation, important evolutionary events and genetic communication frequencies. Additionally, intraspecific nuclear markers also can be used to validate hybrids in controlled crosses, develop genetic maps and conduct marker assisted breeding.
When the chloroplast sequences of TK, TO, and TB were compared, it became apparent that TK and TB were highly similar. Moreover, all four gel-based markers that could discriminate TK and TO could not distinguish TK from TB. These results suggested that TB and TK might share a maternal ancestor. This result supports the finding that the triploid genome of TB is composed of two copies of the TK genome with one copy of an unknown dandelion species (personal communication with Dr. J. Kirschner, Institute of Botany, Academy of Sciences, 25243, Průhonice 1, Czech Republic, 2010). This study may enable additional research on Taraxacum chloroplast diversity, by demonstrating a complement of primers that can amplify entire chloroplast genomes. Furthermore, it may inform chloroplast sequencing efforts to resolve Taraxacum phylogenies, by revealing which regions may have higher interspecific diversity.
The complete annotated chloroplast sequences for TK, TO, and TB allows the development of chloroplast engineering within Taraxacum. The availability of the native chloroplast sequences of an organism can allow constructs to be designed to more readily achieve homologous recombination . Chloroplast engineering is a powerful tool that provides a high level of transgene expression because of the polyploid nature of chloroplast genomes and the large number of chloroplasts present in a single plant cell . Furthermore, chloroplast engineering should prevent the escape of transgenes via pollen, as chloroplasts are maternally inherited [54, 55]. Chloroplast engineering also allows multigene transformation and chloroplast gene manipulation . This research may enable chloroplast engineering in TK and TB to divert additional assimilate to rubber production .
The chloroplast sequences obtained from multiple genotypes within each of the three dandelion species investigated, along with online EST data, allowed us to develop species-specific and intraspecific molecular markers. The availability of chloroplast genomes of these three dandelion species, as well as chloroplast and nuclear molecular markers, provides a powerful genetic resource for germplasm differentiation and purification, and the study of potential gene flow among Taraxacum species. This will further facilitate ecological, evolutionary, and genetic engineering studies for these three species, and significantly accelerate the development of TK as a domestic rubber-producing crop.
Amplified fragment length polymorphism
Cleaved amplified polymorphic sequences
Cetyl trimethylammonium bromide
Dual Organellar GenoMe Annotator
Expressed sequence tag
Large single copy
The molecular and cellular imaging center
National Center for Biotechnology Information
Ohio Agricultural Research and Development Center
Open reading frame
Polymerase chain reaction
Single nucleotide polymorphism
Small single copy
van Beilen JB, Poirier Y. Guayule and Russian dandelion as alternative sources of natural rubber. Crit Rev Biotechnol. 2007;27(4):217–31.
Edathil TT. South American leaf blight‐A potential threat to the natural rubber industry in Asia and Africa. Trop Pest Manag. 1986;32(4):296–303.
Kirschner J, Štěpánek J, Černý T, Heer PD, van Dijk PJ. Available ex situ germplasm of the potential rubber crop Taraxacum kok-saghyz belongs to a poor rubber producer, T brevicorniculatum (Compositae-Crepidinae). Genet Resour Crop Evol. 2013;60(2):455–71.
Krotkov G. A review of literature on Taraxacum koksaghyz Rod. Bot Rev. 1945;11(8):417–61.
Whaley WG, Bowen JS. Russian dandelion (kok-saghyz): an emergency source of natural rubber (US Department of Agriculture). 1947.
Post J, van Deenen N, Fricke J, Kowalski N, Wurbs D, Schaller H, Eisenreich W, Huber C, Twyman RM, Prüfer D, Gronover CS. Laticifer-specific cis-prenyltransferase silencing affects the rubber, triterpene, and inulin content of Taraxacum brevicorniculatum. Plant Physiol. 2012;158(3):1406–17.
van Deenen N, Bachmann A-L, Schmidt T, Schaller H, Sand J, Prüfer D, Gronover CS. Molecular cloning of mevalonate pathway genes from Taraxacum brevicorniculatum and functional characterization of the key enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Mol Biol Rep. 2012;39(4):4337–49.
Schmidt T, Hillebrand A, Wurbs D, Wahler D, Lenders M, Schulze Gronover C, Prüfer D. Molecular cloning and characterization of rubber biosynthetic genes from Taraxacum kok-saghyz. Plant Mol Biol Report. 2009;28(2):277–84.
Plants Profile for Taraxacum officinale (common dandelion). http://plants.usda.gov/core/profile?symbol=taof. Accessed 17 May 2016.
Lyman JC, Ellstrand NC. Clonal diversity in taraxacum officinale (Compositae), an apomict. Heredity. 1984;53(1):1–10.
Solbrig OT. The population biology of dandelions. Am Sci. 1971;59:686–94.
van Dijk P, van Damme J. Apomixis technology and the paradox of sex. Trends Plant Sci. 2000;5(2):81–4.
Ellstrand NC. Dangerous liaisons?: when cultivated plants mate with their wild relatives. Baltimore: Johns Hopkins University Press; 2003.
Morita T, Menken SBJ, Sterk AA. Hybridization between European and Asian dandelions (Taraxacum section Ruderalia and section Mongolica). New Phytol. 1990;114(3):519–29.
Tas IC, Van Dijk PJ. Crosses between sexual and apomictic dandelions (Taraxacum) I. The inheritance of apomixis. Heredity. 1999;83:707–14.
Scarcelli N, Barnaud A, Eiserhardt W, Treier UA, Seveno M, d’Anfray A, Vigouroux Y, Pintaud J-C. A set of 100 chloroplast DNA primer pairs to study population genetics and phylogeny in monocotyledons. PLoS ONE. 2011;6(5):e19954.
Shibaike H, Akiyama H, Uchiyama S, Kasai K, Morita T. Hybridization between European and Asian dandelions (Taraxacum section Ruderalia and section Mongolica). J Plant Res. 2002;115(5):321–8.
Wittzell H. Chloroplast DNA, variation and reticulate evolution in sexual and apomictic sections of dandelions. Mol Ecol. 1999;8(12):2023–35.
Kumar S, Hahn FM, McMahan CM, Cornish K, Whalen MC. Comparative analysis of the complete sequence of the plastid genome of Parthenium argentatum and identification of DNA barcodes to differentiate Parthenium species and lines. BMC Plant Biol. 2009;9(1):131.
Schroeder H, Fladung M. Differentiation of Populus species by chloroplast SNP markers for barcoding and breeding approaches. iForest Biogeosciences For. 2015;8(4):544–6.
Singh N, Choudhury DR, Singh AK, Kumar S, Srinivasan K, Tyagi RK, Singh NK, Singh R. Comparison of SSR and SNP markers in estimation of genetic diversity and population structure of Indian rice varieties. PLoS One. 2013;8(12):e84136.
Barker MS, Kane NC, Matvienko M, Kozik A, Michelmore RW, Knapp SJ, Rieseberg LH. Multiple paleopolyploidizations during the evolution of the Compositae reveal parallel patterns of duplicate gene retention after millions of years. Mol Biol Evol. 2008;25(11):2445–55.
Lai Z, Kane NC, Kozik A, Hodgins KA, Dlugosch KM, Barker MS, Matvienko M, Yu Q, Turner KG, Pearl SA, et al. Genomics of Compositae weeds: EST libraries, microarrays, and evidence of introgression. Am J Bot. 2012;99(2):209–18.
Schuler MA, Zielinski RE. Methods in plant molecular biology. San Diego: Academic; 1989.
Palmer JD. 6 - Isolation and structural analysis of chloroplast DNA. In Methods for Plant Molecular Biology, A W Weissbach. San Diego: Ed Academic Press; 1988. p. 105–124.
Hellier BC. Collecting in Central Asia and the Caucasus: U.S. National Plant Germplasm System plant explorations. HortSci. 2011;46:1438–9.
Germplasm Resources Information Network (GRIN). http://www.ars-grin.gov/npgs/. Accessed 2 Aug 2016.
Kabelka E, Franchino B, Francis DM. Two loci from Lycopersicon hirsutum LA407 confer resistance to strains of Clavibacter michiganensis subsp michiganensis. Phytopathology. 2002;92(5):504–10.
FASTX-Toolkit: FASTQ/A short-reads pre-processing tools. http://hannonlabcshledu/fastx_toolkit/index.html. Accessed 3 May 2014.
Zerbino DR, Birney E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008;18(5):821–9.
Madden T. The BLAST Sequence Analysis Tool. In: McEntyre J, Ostell J, editors. The NCBI Handbook [Internet]. Bethesda: National Center for Biotechnology Information (US); 2002. Chapter 16. 2003. http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/books/NBK21097/. Accessed 10 May 2014.
Wyman SK, Jansen RK, Boore JL. Automatic annotation of organellar genomes with DOGMA. Bioinformatics. 2004;20(17):3252–5.
Lohse M, Drechsel O, Kahlau S, Bock R. OrganellarGenomeDRAW-a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res. 2013;41:W575–81.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013;30:2725–9.
Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10(3):512–26.
Frazer KA, Pachter L, Poliakov A, Rubin EM, Dubchak I. VISTA: computational tools for comparative genomics. Nucleic Acids Res. 2004;32:W273–9.
Mayor C, Brudno M, Schwartz JR, Poliakov A, Rubin EM, Frazer KA, Pachter LS, Dubchak I. VISTA: visualizing global DNA sequence alignments of arbitrary length. Bioinformatics. 2000;16(11):1046–7.
Brudno M, Malde S, Poliakov A, Do CB, Couronne O, Dubchak I, Batzoglou S. Glocal alignment: finding rearrangements during alignment. Bioinformatics. 2003;19:i54–62.
Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012;9(4):357–9.
Garrison E, Marth G. Haplotype-based variant detection from short-read sequencing. E-prints arXiv:1207.3907v2 [q-bio.GN]. 2012. https://arxiv.org/abs/1207.3907v2. Accessed 21 Dec 2012.
Kozik A. CDS assembly and SNP discovery. http://cgpdbucdavisedu/SNP_Discovery_CDS/. Accessed 2 Dec 2012.
Koressaar T, Remm M. Enhancements and modifications of primer design program Primer3. Bioinformatics. 2007;23(10):1289–91.
Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG. Primer3-new capabilities and interfaces. Nucleic Acids Res. 2012;40:e115.
Fernandez-Pozo N, Menda N, Edwards JD, Saha S, Tecle IY, Strickler SR, Bombarely A, Fisher-York T, Pujar A, Foerster H, et al. The Sol Genomics Network (SGN)-from genotype to phenotype to breeding. Nucleic Acids Res. 2014;43:D1036–41.
Ye S, Dhillon S, Ke X, Collins AR, Day INM. An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 2001;29(17):e88.
NCBI Organelle Genome Resources Database. http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/genome/organelle/. Accessed 11 Apr 2016.
Choi KS, Park S. The complete chloroplast genome sequence of Aster spathulifolius (Asteraceae); genomic features and relationship with Asteraceae. Gene. 2015;572(2):214–21.
Kim KJ, Choi KS, Jansen RK. Two chloroplast DNA inversions originated simultaneously during the early evolution of the sunflower family (Asteraceae). Mol Biol Evol. 2005;22(9):1783–92.
Timme RE, Kuehl JV, Boore JL, Jansen RK. A comparative analysis of the Lactuca and Helianthus (Asteraceae) plastid genomes: identification of divergent regions and categorization of shared repeats. Am J Bot. 2007;94(3):302–12.
Koch MA. Evolution of the trnF (GAA) gene in Arabidopsis relatives and the Brassicaceae family: monophyletic origin and subsequent diversification of a plastidic pseudogene. Mol Biol Evol. 2005;22(4):1032–43.
Kim J-K, Park JY, Lee YS, Lee HO, Park H-S, Lee S-C, Kang JH, Lee TJ, Sung SH, Yang T-J. The complete chloroplast genome sequence of the Taraxacum officinale F.H.Wigg (Asteraceae). Mitochondrial DNA Part B. 2016;1:228–9.
Verma D, Samson NP, Koya V, Daniell H. A protocol for expression of foreign genes in chloroplasts. Nat Protoc. 2008;3(4):739–58.
Bendich AJ. Why do chloroplasts and mitochondria contain so many copies of their genome? BioEssays. 1987;6(6):279–82.
Daniell H. Molecular strategies for gene containment in transgenic crops. Nat Biotechnol. 2002;20(6):581–6.
Scott SE, Wilkinson MJ. Low probability of chloroplast movement from oilseed rape (Brassica napus) into wild Brassica rapa. Nat Biotechnol. 1999;17(4):390–2.
Daniell H, Kumar S, Dufourmantel N. Breakthrough in chloroplast genetic engineering of agronomically important crops. Trends Biotechnol. 2005;23(5):238–45.
Kumar S, Hahn FM, McMahan CM, Cornish K, Whalen MC. Remodeling the isoprenoid pathway in tobacco by expressing the cytoplasmic mevalonate pathway in chloroplasts. Metab Eng. 2012;14(1):19–28.
We thank Dr. Tea Meulia, Dr. Asela Wijeratne, Saranga Wijeratne and Maria Elena Hernandez-Gonzalez from The Molecular and Cellular Imaging Center (MCIC) in Ohio Agricultural Research and Development Center (OARDC) for their sequencing and assembly support, and Dr. Wenshuang Xie and Dr. Joshua Blakeslee for their assistance in the chloroplast DNA preparation.
We thank USDA National Institute of Food and Agriculture (Hatch project 230837) and OSU OARDC SEEDS grant 2011-004 for financial support.
Availability of data and material
The complete chloroplast genomes of TK, TO, and TB were submitted to the NCBI database with GenBank accession numbers KX198560 (TK), KX198561 (TO), and KX198559 (TB). Other data used in the analysis are included within the article and the additional files.
KC coordinated the study. YZ, BJI and KC designed the experiments. YZ and BJI prepared the chloroplast DNA samples for sequencing. YZ carried out genome annotation, comparison analysis and marker validation. BJI assembled nuclear EST data and discovered nuclear SNP markers. XZ assembled chloroplast genomes, conducted chloroplast SNP calling and phylogenetic analysis. JC provided the plant materials for this study. YZ and XZ prepared figures and tables. All authors participated in the manuscript writing and gave final approval for publication.
The authors declare that they have no competing interests.
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Ethics approval and consent to participate
Availability of supporting data
The phylogenetic data was deposited in the Treebase Repository and available at: http://purl.org/phylo/treebase/phylows/study/TB2:S20241.
Taraxacum kok-saghyz genotypes for sequencing and marker validation. (DOCX 28 kb)
Taraxacum officinale genotypes for sequencing and marker validation. (DOCX 47 kb)
Taraxacum brevicorniculatum genotypes for sequencing and marker validation. (DOCX 28 kb)
Primers used for chloroplast genome amplification by Long Range PCR. (DOCX 27 kb)
Rubisco large subunit genes (rbcL) from the Asteraceae. (DOCX 29 kb)
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Zhang, Y., Iaffaldano, B.J., Zhuang, X. et al. Chloroplast genome resources and molecular markers differentiate rubber dandelion species from weedy relatives. BMC Plant Biol 17, 34 (2017) doi:10.1186/s12870-016-0967-1
- Chloroplast genome
- Species-specific single nucleotide polymorphism markers
- Taraxacum brevicorniculatum
- Taraxacum kok-saghyz
- Taraxacum officinale