S. No | Gene | NCBI Genbank | Gene size (kb) | Gene area of highest PSSM Score difference | PSSM Score difference | No of amplicons used to cover EcoTILLING fragments | No of exons covered | No of splice sites covered | Function | Consequence of disruption | |
---|---|---|---|---|---|---|---|---|---|---|---|
mRNA ID | Genomic ID | ||||||||||
1. | GBSS I | AB425323.1 | NC_008399.2 | 3.479 | 1679 to 2977 | 2782 | 1 | 8 | 7 | Amylose biosynthesis | Down or up regulation of amylose |
2. | SS I | GQ150950.1 | NC_008399.2 | 6.815 | 4550 to 5848 | 1708 | 1 | 8 | 7 | Amylopectin synthesis | Branching pattern of amylopectin is likely to be changed, no effect on amylose |
3. | SS IIa | GQ150968.1 | NC_008399.2 | 4.419 | 3037 to 4335 | 3346 | 2 | 3 | 1 | Amylopectin synthesis | Branching pattern of amylopectin is likely to be changed, Increase in amylose |
4. | SS IIIa | GQ151010.1 | AK061604 | 11.083 | 8887 to10185 | 3203 | 2 | 7 | 5 | Amylopectin synthesis | Branching pattern of amylopectin is likely to be changed, moderate increase in AC, no effect on RS |
5. | SBE Ia | GQ150912.1 | NC_008399.2 | 4.745 | 923 to 2221 | 2384 | 1 | 5 | 4 | Amylopectin synthesis | Preferentially branches amylose type polyglucans. And it has capacity for branching less branched α-glucans. Also modifies amylopectin architecture and grain morphology. |
6. | SBE IIb | D16201.1 | NC_008395.2 | 10.899 | 3517 to 4815 | 1711 | 2 | 13 | 11 | Amylopectin synthesis | Increased amylose and RS concentration and alter amylopectin architecture such as degree of branching and branching pattern |