Fig. 1
From: Evolutional dynamics of 45S and 5S ribosomal DNA in ancient allohexaploid Atropa belladonna
Karyological characterization of Atropa belladonna (accessions 986 and 987). a and b Double fluorescent staining with 4′,6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA), respectively; c Localization of 5S and 45S rDNA sequences on chromosomes; The chromosomes were stained by DAPI (blue fluorescence), hybridization signals of 5S (red) and 45S rDNA (green) are marked by arrows; d Active 45S rDNA (nucleolar organizing region, NOR) sites (arrows) in Atropa belladonna detected by silver staining; Scale bar, 10 μm