Fig. 6

Predicted targets of miR166 family and validation of miR166 target gene using RLM-RACE PCR. a The alignments of fragments of target mRNAs that have complementarity to miR166s are shown. miR166 sequences are displayed to highlight complementarity to target mRNAs. The targets are classified into seven groups (I, no annotation; II, ATHB-15; III, ATHB14-LIKE; IV, REVOLUTA-LIKE; V, PHYTOENE SYNTHASE; VI, AMMONIUM TRANSPORTER 2-LIKE; VII, two-component response regulator-like APRR2). b RLM-RACE PCR for ATHB14-LIKE. Lane 1 and M show cleaved product of desired size (734 bp) and DNA ladder, respectively. c Mapping of ATHB14-LIKE mRNA cleavage sites by RLM-RACE. The arrows indicate the cleavage sites and the numbers show the frequency of clones sequenced