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Fig. 2 | BMC Plant Biology

Fig. 2

From: Novel Arabidopsis microtubule-associated proteins track growing microtubule plus ends

Fig. 2

Recombinant GPT1 and GPT2 bind to MTs in vitro. (a) Purified MBP-fused GPT1 and GPT2 proteins were incubated with or without taxol-stabilized MTs, pelleted by ultracentrifugation, and analyzed by SDS-PAGE and Coomassie Brilliant Blue staining. The positions of full-length MBP-GPT1 and MBP-GPT2 are indicated by arrowheads, and the MT-binding partial degradation products of MBP-GPT1 are indicated with an asterisk. Tubulin is also indicated. S, supernatant fraction; P, pellet fraction; and M, size markers. (b) Quantitative analysis of the binding of MBP-GPT2 to MTs. Various concentrations of purified MBP-GPT2 was mixed with taxol-stabilized MTs and then subjected to co-sedimentation analysis, as in A. Assuming that there is one GPT2 binding site on the tubulin dimer, the binding equation (see Methods) was fitted

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