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Fig. 4 | BMC Plant Biology

Fig. 4

From: Novel Arabidopsis microtubule-associated proteins track growing microtubule plus ends

Fig. 4

GPT1 tracks the growing plus ends of cortical MTs. a The subcellular localization of GFP-GPT1 (green) and mCherry-TUB6, which labels cortical MTs (magenta), was analyzed in interphase cells of the Arabidopsis root epidermis. Scale bar, 5 μm. b Average fluorescence intensity (FI) profiles of GFP-GPT1 and mCherry-TUB6 were obtained by analyzing and plotting data from 20 MT images. The data were normalized and the peak intensity of GFP-GPT1 was set to 1. The error bars indicate SEM. c Representative time-lapse sequence and corresponding kymograph of a MT that underwent catastrophe at t = 14 s. GFP-GPT1 disappeared from the tip immediately after the MT began to depolymerize. Dashed yellow arrows indicate the point at catastrophe occurred. Scale bar, 2.5 μm. d Representative time-lapse sequence and corresponding kymograph of a MT that underwent rescue at t = 12 s. GFP-GPT1 localized to the tip immediately after MT growth resumed. Dashed yellow arrows indicate the point at which rescue occurred. Scale bar, 2.5 μm

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