Fig. 4
Effect of silencing of GaRPL18 in cotton under V. dahliae stress. a The observation of cell morphology of the stems of CLCrV and CLCrV:GaRPL18 cotton plants at 25 dpi. b Trypan blue staining was used to visualize cell death of the leaves of CLCrV and CLCrV:GaRPL18 cotton plants at 25 dpi. c Measuring the SA content of CLCrV and CLCrV:GaRPL18 cotton plants at 25 dpi via HPLC. Bars represent standard error from three biological replicates. Asterisks indicate statistically significant differences (Student’s t-test; *P <0.05). d Leaves of CLCrV and CLCrV:GaRPL18 cotton plants were harvested at 12 hpi and 24 hpi to measure the contents of NO, H2O2 and CAT. Error bars represent the standard deviation of three biological replicates. Asterisks indicate statistically significant differences when compared with control (Student’s t-test; *P <0.05; **P <0.001)