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Fig. 5 | BMC Plant Biology

Fig. 5

From: Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable

Fig. 5

Relative specific activities of NbXIP1;1α mutants for water permeability in P. pastoris spheroplasts. The specific activities of the individual NbXIP1;1α mutants and the wild-type NbXIP1;1α (NbXIP1;1αwt) were normalized to the specific activity of the NbXIP1;1αL79G/I102H/V242I mutant. The background corrected rate constants obtained from the osmotic water permeability stopped flow spectroscopy assay were divided by the individual protein amounts estimated by western blot to obtain the specific activities. a First set of NbXIP1;1α mutants. NbXIP1;1αL79G/I102H/V242I, NbXIP1;1αL79G/I102H/V242I/ΔC, NbXIP1;1αL79G/I102H/V242I/ΔD and NbXIP1;1αL79G/I102H/V242I/ΔC/ΔD were designed based on the ICVAR alignment of NbXIPs helix 5 while NbXIP1;1αL79G/I102H/T246I, NbXIP1;1αL79G/I102H/T246I/ΔC and NbXIP1;1αL79G/I102H/T246I/ΔD were designed based on the ICTAR alignment. a and c indicate statistical significant differences (P < 0.05), whereas b is not significant different. See Additional file 5: Table S4. ΔC and ΔD indicate truncations in loop C and loop D. b Second set of NbXIP1;1α mutants. Comparisons marked a and b, d, f, g indicate statistical significant differences at two different levels (P < 0.0005 and P < 0.05, respectively) while differences between pairs marked c and e are not significant. See Additional file 6: Table S5

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