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Fig. 7 | BMC Plant Biology

Fig. 7

From: Root morphogenic pathways in Eucalyptus grandis are modified by the activity of protein arginine methyltransferases

Fig. 7

Western blot of un-treated E. grandis roots and roots transgenically mis-expressing EgPRMT1 and EgPRMT10. a Western blots of whole cell lysates from E. grandis roots transformed with A. rhizogenes strain K599 (control) or roots over-expressing EgPRMT1 or EgPRMT10. Blots were probed with anti-ASYM24. Equal amounts of total protein were migrated in each sample and β-tubulin was used to confirm normalization of total protein input. * = bands showing increased intensity from controls in multiple experiments. b Western blot of protein from E. grandis roots transformed with A. rhizogenes strain K599 (control) or 35S::EgPRMT1 immuno-precipitated with the anti-β-tubulin antibody. Blots were probed with anti-ASYM24 or anti-β-tubulin. Graph shows relative amount of methylated β-tubulin compared to total β-tubulin based on Western blot band intensity. * = significant increase in band intensity (p < 0.05)

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