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Fig. 1 | BMC Plant Biology

Fig. 1

From: Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm

Fig. 1

Schematic representation of the plant expression vectors for the expression of the BP178 gene in the rice endosperm. a Three different constructs in pCAMBIA vector (pC) in which the synthetic gene was cloned between the endosperm-specific promoter including the signal peptide coding sequence of the corresponding seed storage protein (SP) and the nopaline synthase terminator (nos). Relevant restriction enzyme sites for cloning purposes are indicated. The hptII gene encoding resistance to hygromycin under the control of the CaMV35S promoter and terminator was contained into the T-DNA region of pC. LB, left border; RB, right border of T-DNA. Arrows indicate the orientation of the sequence. b The chimeric BP178 peptide corresponding to the fusion of BP134 (KKLFKKILKYL-OH, a cecropin A (1–7)-melittin (2–9) hybrid, in green) linked through the hinge sequence (AGPA, in orange) to a magainin fragment peptide (GIGKFLHSAKKFGKAFVGEIMNS –OH, in blue); and extended with ER retention signal (KDEL, in pink). For details, see Additional file 1: Figure S1

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