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Fig. 4 | BMC Plant Biology

Fig. 4

From: Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm

Fig. 4

Purification, quantification and antibacterial activity of the BP178 peptide recovered from the pGlb1:BP178 rice seeds. a Schematic procedure for purification. BP178 peptide was recovered at high purity in the acetone supernatant (2) b Tris-Tricine SDS-PAGE and western-blot analysis using anti-BP178 antibodies of fractions recovered during the purification process described in (a) starting from protein body fractions (50 μg) from seeds from pGlb1:BP178 line 7.2. Numbers in the top of panel (b) correspond to: 1, Supernatant; 2, Pelleted protein bodies; 3, Pellet. c Peptide yield from seeds of the indicated pGlb1:BP178 events. The amount of BP178 was calculated by comparing the signal intensity of known amounts of standard (synthetic peptide). Values correspond to the mean of at least 3 independent assays and the standard error is indicated. d Antimicrobial activity against Dickeya sp. of two batches of purified BP178-fractions from pGlb1:BP178 lines. Empty vector (EV) and reference synthetic BP178 peptide were used as control. Mean values of two replicates and the standard error are indicated

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