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Table 1 Primer sequences for RT-PCR and qRT-PCR of anthocyanin biosynthetic genes

From: Overexpression of snapdragon Delila (Del) gene in tobacco enhances anthocyanin accumulation and abiotic stress tolerance

Gene

Accession No.

Primer sequences

CHS

AF311783

F, 5′- TTGTTCGAGCTTGTCTCTGC −3′

R, 5′- AGCCCAGGAACATCTTTGAG-3′

CHI

AB213651

F, 5′- GTCAGGCCATTGAAAAGCTC −3′

R, 5′- CTAATCGTCAATGCCCCAAC −3′

F3H

AB289450

F, 5′- CAAGGCATGTGTGGATATGG-3′

R, 5′- TGTGTCGTTTCAGTCCAAGG −3′

DFR

AB289448

F, 5′- AACCAACAGTCAGGGGAATG −3′

R, 5′- TTGGACATCGACAGTTCCAG-3′

ANS

AB289447

F, 5′- TGGCGTTGAAGCTCATACTG −3′

R, 5′- GGAATTAGGCACACACTTTGC −3′

ACTIN

AB158612

F, 5′- GGTCATTACCATTGGCTCAGA −3′

R, 5′- CAACAAGTGATGGCTGGAATAAA −3′

  1. CHS chalcone synthase, CHI chalcone isomerase, F3H flavanone-3-hydroxylase, DFR dihydroflavonol 4-reductase, ANS anthocyanidin synthase, ACTIN reference gene used for expression normalization
  2. PCR conditions for RT-PCR: 95 °C (2 min) → [95 °C (20 s) → 57 °C (40 s) → 72 °C (30 s)] × 35 cycles → 72 °C (5 min) → 4 °C
  3. PCR conditions for qRT-PCR: 95 °C (10 min) → [95 °C (30 s) → 57 °C (25 s) → 72 °C (35 s)] × 40 cycles → 95 °C (15 s) → 57 °C (30 s) → 95 °C (15 s)
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BMC Plant Biology

ISSN: 1471-2229

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