Fig. 8

This cartoon summarizes the events occurring during the two hours required to harvest, surface sterilize, and plate 3 DAP kernels on medium. In (a), kernels are on the plant being fed on sucrose obtained from photosynthesis. Cellular sucrose (20 μmol∙g⁻¹ FW) and Tre6P levels are high (54 nmol∙g⁻¹ FW) and SnRK1 activity is low, as is the transcription of putative SnRK1 target genes, class II TPS genes, TPP genes, INCW2 and SuSy1. Two hours after ears are removed from the plant (b) Tre6P levels drop below 1 nmol∙g⁻¹ FW, SnRK1 activity is high, and the transcription increases for putative SnRK1 target genes, class II TPS genes, TPP genes, INCW2 and SuSy1