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Fig. 6 | BMC Plant Biology

Fig. 6

From: TNO1, a TGN-localized SNARE-interacting protein, modulates root skewing in Arabidopsis thaliana

Fig. 6

The orientation of MT arrays in the elongating cells of skewing mutant roots is not affected. a Representative confocal images of the cortical microtubule array of elongating cells of 5-day-old Arabidopsis roots grown on slanted medium, immunostained with anti-α- tubulin. The boxed inset indicates the region that was selected for the MT array analysis in 6b. Scale bar =10 μm. b Graphical representation of the analyzed MTs as a circular plot derived from analysis of the confocal images in part a with the MicroFilament Analyzer software (MFA). The blue line represents the original data (the MT angles) while the red line represents smoothed data. Green asterisks denote dominant orientations while the yellow line is a weighted average of the detected filaments per angle. c Microtubule angle distribution in the elongating cells of WT and KO roots was calculated from the data generated via the MFA software. Values represent analysis of 3 biological replicates with at least 500 MTs analyzed from 3 cells of three different roots for each replicate. Error bars indicate standard errors

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