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Fig. 7 | BMC Plant Biology

Fig. 7

From: Reactive oxygen species mediate tapetal programmed cell death in tobacco and tomato

Fig. 7

Pharmacological interference of ROS amplitude compromised tapetal degeneration and pollen development. a Representative NBT staining of tobacco anthers at different developmental stages from floral buds treated with DMSO or with 10 μM DPI. b Transverse sections of tobacco anthers at the stage of meiosis, microspore, mitosis, or dehiscence from DMSO- or DPI-treated flowering buds. Arrowheads point to the tapetal layer. Insets are close-ups showing the tapetal layer (highlighted with doted lines, DPI-treated) or its absence (DMSO-treated). c PI staining of mature pollen grains released from tobacco flowers treated with DMSO or with DPI during anther development. Transmission image is shown at the right side of its corresponding fluorescence image. Asterisks indicate aborted pollen grains. Numbers at the top are means ± standard deviation (SD, N = 20–25). DMSO and DPI treatments are significantly different (t-test, P < 0.01). Bars, 500 μm for (a); 50 μm for (b, c); 10 μm for (b inset)

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