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Table 4 Primers used in these studies

From: Identification and biochemical characterization of the fructokinase gene family in Arabidopsis thaliana

Cloning primers

AGI Number of target and primer direction

Purpose

Primer sequence (5′- -3′)

At2g31390 – forward

FRK2 bacterial/plant expression

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGATGGCATCCAACGGTGATAAAGG

At2g31390 – reverse

FRK2 bacterial expression

GGGGACCACTTTGTACAAGAAAGCTGGGTCCTATTTCTTTTCGAG

At1g66430 – forward

FRK3 bacterial expression/removal of cTP

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGTCTAATCTCAAAGGAAGAGC

At1g66430 – reverse

FRK3 bacterial expression

GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAAACGACGGCTTTGAG

At4g10260 – forward

FRK4 bacterial/plant expression

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGATGGCTAATACTCCATTG

At4g10260 – reverse

FRK4 bacterial expression

GGGGACCACTTTGTACAAGAAAGCTGGGTCCTATTTAGACTTAGATTTC

At1g06030 – forward

FRK6 bacterial/plant expression

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGATGACGTCATCCAACGGCG

At1g06030 – reverse

FRK6 bacterial expression

GGGGACCACTTTGTACAAGAAAGCTGGGTCCTATTCTACTTGTATCTTAAG

At3g59480 – forward

FRK7 bacterial/plant expression

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGATGGCTGCATCTAACGGCGAG

At3g59480 – reverse

FRK7 bacterial expression

GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGTTTCCTTTCAGGAGGC

At5g51830 – forward

FRK1 plant expression

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGATGGGTGAGGATGCAATCTC

At5g51830 – reverse

FRK1 plant expression stop codon mutation

GGGGACCACTTTGTACAAGAAAGCTGGGTCTGACGATCGAGTAGAAGAAAG

At2g31390 – reverse

FRK2 plant expression stop codon mutation

GGGGACCACTTTGTACAAGAAAGCTGGGTCCCATTTCTTTTCGAG

At1g66430 – forward

FRK3 plant expression

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGATGGCTCTCCAAGCCACTAC

At1g66430 – reverse

FRK3 plant expression stop codon mutation

GGGGACCACTTTGTACAAGAAAGCTGGGTCTGAAACGACGGCTTTGAG

At4g10260 – reverse

FRK4 plant expression stop codon mutation

GGGGACCACTTTGTACAAGAAAGCTGGGTCCGATTTAGACTTAGATTTC

At1g06020 – forward

FRK5 plant expression

GGGGACAAGTTTGTACAAAAAAGCAGGCTTGATGGCATCATCCACCGGCG

At1g06020 – reverse

FRK5 plant expression stop codon mutation

GGGGACCACTTTGTACAAGAAAGCTGGGTCTGAACAAGGgctAGCCGTACAACACC

At1g06030 – reverse

FRK6 plant expression stop codon mutation

GGGGACCACTTTGTACAAGAAAGCTGGGTCCGATTCTACTTGTATCTTAAG

At3g59480 – reverse

FRK7 plant expression stop codon mutation

GGGGACCACTTTGTACAAGAAAGCTGGGTCTGAGTTTCCTTTCAGGAGGC

Primers for analysis of T-DNA insertional mutants

Arabidopsis T-DNA line

Primer target

Primer sequence (5′- -3′)

frk1–1 (SALK_046463)

gDNA

CTGGGATGAGATTCGACCAT

gDNA/T-DNA

GGCAAGGTTCCTCAATCAAA

cDNA flanking insert

ATGTTAGCTGATATTCTAAG

cDNA flanking insert

TAGCAGCTTCTTCTGATGGC

frk2–1 (SALKseq_17726)

gDNA/T-DNA

TCATGGCGAGGATCTTTTGCT

gDNA

CACACTAGTTTTGCCTTCTGGT

cDNA flanking insert

TAGTGGAGCCGTGTAGGTCA

cDNA flanking insert

AGCTCAACATCGCTCACCTT

frk2–2 (SALK_114786)

gDNA 5’

TTCTTCTCCTTGTCCTTCCTT

gDNA/ T-DNA 3’

CGAGACGAGAAACAGCGATT

cDNA flanking insert

CTTCGGCGAGATGCTAATCG

cDNA flanking insert

TCCCAGCCAACATATGACCG

frk3–1 (SALK_044085)

gDNA

CGTCCTATCTCCATCAAAGC

gDNA/T-DNA

CGCTTGTCGTCGGTACAA

cDNA flanking insert

GTTTTACCGAAACCCGAGTG

cDNA flanking insert

CCGCCTTAGCTGCAGAAATA

frk3–2 (SALK_035386)

gDNA

CCGCCTTAGCTGCAGAAATA

gDNA/T-DNA

GTTTTACCGAAACCCGAGTG

cDNA flanking insert

GTTTTACCGAAACCCGAGTG

cDNA flanking insert

CCGCCTTAGCTGCAGAAATA

frk5–1 (SALK_027635)

gDNA

CGCTTTTGTCACTTTGCGTTCTGAC

frk5–2 (SALK_057002)

gDNA/T-DNA

CGAGTACGGACTGGTCATCAACAA

cDNA flanking insert

GATGATTTCGGTCATATGCTCGC

cDNA flanking insert

GGAGAAGCCGTACAACACCA

frk7–1 (GABI_253H07)

gDNA

CAGCACGGTCATCAACAATC

gDNA/T-DNA

TAGCGAACGGTGCTACATCA

T-DNA (GABI)

ATATTGACCATCATACTCATTGC

cDNA flanking insert

AACGGTGTCTCTGCTGAAGG

cDNA flanking insert

TCAGGAGGCTCTGAACTTCA

SALK T-DNA Left border

T-DNA in SALK lines

TGGTTCACGTAGTGGGCCATCG

Positive control gene (At1g49350)

cDNA

GCGTGGATGCCGTTGAAAAT