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Table 1 UV/vis spectra and characteristic ions of carotenoids from six maturation stages of loquat fruits, obtained by HPLC-PDA-MS

From: Metabolic and transcriptional elucidation of the carotenoid biosynthesis pathway in peel and flesh tissue of loquat fruit during on-tree development

Peak Carotenoid tR (min) λmax (nm) %III/II %Ab/II [M + H] + m/z HPLC/APCI (+)-MSn experiment m/z (% base peak) Exact mass Score Error (ppm) Molecular formula
1 All-trans-neoxanthin 14.1 412,436,462 70 0 601 (40), 583 (75), 565 (100) MS2 [601]: 583 (100), 565 (47), 547 (9), 509 (6),491 (5), 221 (41)
MS3 [601˃583]: 565 (48), 547 (14)
600.4188 91.88 -2.69 C40H56O4
2 All-trans-neochrome 14.6 397,420,448 90 0 601 (42), 583 (100), 565 (43) MS2 [601]: 583 (100), 565 (57),
MS3 [601˃583]:221 (62)
600.4178 96.70 -0.89 C40H56O4
3 All-trans-violaxanthin 14.8 414,438,470 75 0 601 (85), 583 (100), 565 (20) MS2 [601]: 583 (100), 565 (12), 509 (5), 221 (24) 600.4178    C40H56O4
4 Not identified 16.0 466 0 0 455 (100) MS2 [455]: 437 (80), 399 (34) 455.3324 93.60 -2.61 C33H43O
5 Not identified 16.2 396,420,448 75 0 601 (60) MS2 [601]: 583 (95), 221 (100     
6 Not identified 16.4 Not detected -- -- 601 (64) MS2 [601]: 583 (90), 565 (40), 491 (9), 221 (100)
MS3 [601˃583]:565 (100),221 (60)
    
7 β- Diepoxy-cryptoxanthin 16.7 412, 436,466 72 0 585 (100) MS2 [585]: 567 (45), 549 (80), 493 (37), 221 (100), 205 (10) 584.4184 95.60 -2.52 C40H56O3
8 Cis-violaxanthin 17.3 324,410,
434,464
60 8 601 (81), 487 (100) MS2 [601]: 583 (100), 565 (42), 509 (6),491 (23), 221 (51)
MS2 [487]: 469 (100)
    
9 Not identified 17.8 378,400,424 100 0 601 (100), 351 (98) MS2 [601]: 583 (100), 565 (13), 509 (15), 491 (14),393 (40),221 (41)
MS2 [351]: 333 (16)
    
10 All-trans-lutein 18.2 420,444,472 48 0 569 (5), 551 (100) MS2[551]: 533 (51), 495 (24), 477 (35) 568.427 99.30 -0.226 C40H56O2
11 Not identified 18.5 444 0 0 454 (100) MS2[454]: 436 (7), 393 (100)     
12 Not identified 18.9 448 0 0 473 (100),539 (80) MS2[539]: 521 (100)     
13 Not identified 19.0 454 0 0 473 (100), 454 (14) MS2 [473]: 455 (43), 205 (100)
MS2 [454]: 436 (31), 393 (36)
    
14 Not identified 19.3 423,438,474 60   551 (100) MS2 [551]: 533 (67), 477 (55)     
15 Not identified 19.4 448 0 0 439 (93), 403 (100) MS2 [439]:403 (14)     
16 Not identified 19.6 434 0 0 391 (100) MS2 [391]:373 (14)     
17 5,6-Epoxy-β-cryptoxanthin 19.8 416,438,466 34 0 569 (17),551 (54) MS2 [551]: 533 (20), 205 (21) 568.428 75.56 -5.07 C40H56O2
18 5′,6′-Epoxy-β-cryptoxanthin 19.9 419,441,470 52 0 569 (30), 551 (100) MS2 [551]: 533 (100), 577 (14), 459 (30), 221 (13) 568.428 89.42 -2.11 C40H56O2
19 Cis-lutein 20 326,412,436,462 52 20 569 (18), 551 (100) MS2 [551]: 533 (100), 221 (11) 568.428 77.7 3.57 C40H56O2
32 β- Diepoxy-cryptoxanthin 20.2 416,440, 470 87 0 585 (100) MS2 [583]: 567 (49), 549 (10) 221 (31), 205 (29)     
20 Not identified 20.4     446 (100) 417 (90) MS2 [417]:399 (100)
MS2 [446]:219 (100)
    
21 β-Apo-8′-carotenal (IS) 21.0 450 0 0 417 (100) MS2 [417]:399 (25), 389 (32), 361 (39), 325 (100), 293 (97), 157 (88), 119 (16) 416.3079 98.23 -2.15 C30H40O
22 Citranaxanthin 21.6 470 0 0 457 (100) MS2 [457]:439 (90), 399 (49) 455.3324 97.23 -1.25 C33H44O
23 Not identified 22.4 470    696 (100) MS2 [696]: 534 (34), 516 (100)     
24 Not identified 22.8 420,444,470 31 0 537 (58) MS2 [537]: 467 (16), 444 (51), 365 (100)     
25 Not identified 23.3 Not detected    537 (28),430 (100) MS2 [537]:444 (100),481 (24) 413 (90)     
26 All-trans-β-cryptoxanthin 24.0 420, 448,472 24 0 553 (100) MS2 [553]:535 (100), 497 (41),461 (10)     
27 Not identified 24.5     664 (100) MS2 [664]: 551 (100), 496 (55)     
28 Not identified 24.9 453, 479 0 0 551 (100) MS2 [551]: 534 (15), 361 (100)     
29 Phytoene + not identified 14 28.0     545 (95), 553 (100) MS2 [545]:489 (10),395 (100)
MS2 [553]:535 (12)
    
30 5,8-epoxy-β-carotene 28.4 405, 424, 450 26 0 553 (100) MS2 [553]:535 (55),461 (35), 221 (64),205 (17)     
31 All-trans-β-carotene 36.5 424, 446,470 18 0 537 MS2 [537]:444 (100)