iTRAQ-based proteome profile analysis of superior and inferior Spikelets at early grain filling stage in japonica Rice

You, Cuicui; Chen, Lin; He, Haibing; Wu, Liquan; Wang, Shaohua; Ding, Yanfeng; Ma, Chuanxi

doi:10.1186/s12870-017-1050-2

Table 2 Identification of 159, 87 and 28 differentially expressed proteins (≥ 1.5 fold) between SS and IS at 10 DAP in group A (T0-SS/T0-IS), B (T0-SS/T2-IS) and C (T2-IS/T0-IS)

From: iTRAQ-based proteome profile analysis of superior and inferior Spikelets at early grain filling stage in japonica Rice

Protein No.	Accession	Uniprot date accession no.	Protein name UniprotKB database	Fold-change (≥1.5-fold)
Protein No.	Accession	Uniprot date accession no.	Protein name UniprotKB database	T0-SS/T0-IS (A)	T0-SS/T2-IS (B)	T2-IS/T0-IS (C)
Cell growth/division
36	Os02g0753800	Q6Z6A7	Annexin	3.01 ± 0.71	ns	ns
91	Os05g0438800	Q75HX0	Actin	2.89 ± 0.12	1.76 ± 0.08	ns
154	Os07g0249700	Q8H3C8	IAA-amino acid hydrolase ILR1-like 8	3.52 ± 0.47	2.06 ± 0.52	ns
Sugar metabolism
14	Os03g0758100	Q9AUV8	Alpha-1,4 glucan phosphorylase	4.70 ± 0.48	3.18 ± 1.26	ns
19	Os06g0194900	P30298	Sucrose synthase 2	6.04 ± 1.05	ns	3.57 ± 1.79
26	Os03g0278000	Q8W3J0	Os03g0278000 protein	0.35 ± 0.04	ns	ns
33	Os03g0703000	Q75I93	Beta-glucosidase 7	0.40 ± 0.02	ns	ns
38	Os01g0944700	Q94CR1	Beta 1,3-glucanase	2.82 ± 0.20	2.45 ± 0.27	ns
50	Os10g0340600	Q7XFK2	Beta-galactosidase 14	0.40 ± 0.13	ns	ns
54	Os02g0752200	Q6Z8I7	Os02g0752200 protein	0.37 ± 0.09	ns	ns
65	Os08g0509200	Q84YK7	Beta-glucosidase 27	0.45 ± 0.02	ns	0.57 ± 0.02
121	Os06g0172800	Q5SNC5	Putative seed imbibition protein	2.52 ± 0.32	ns	ns
136	Os03g0340500	Q10LP5	Sucrose synthase 4	5.38 ± 1.07	2.02 ± 0.19	ns
Starch biosynthesis
6	Os04g0409200	Q0JDF0	Os04g0409200 protein	0.48 ± 0.04	ns	ns
40	Os08g0345800	P15280	Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic/amyloplastic	4.66 ± 0.51	ns	ns
41	Os05g0580000	Q688T8	Glucose-1-phosphate adenylyltransferase	0.21 ± 0.08	0.21 ± 0.08	ns
42	Os01g0130400	Q9LGC6	Putative alpha-glucosidase	0.48 ± 0.04	ns	ns
44	Os01g0633100	Q7G065	ADP-glucose pyrophosphorylase/AGPase	3.36 ± 0.44	ns	ns
45	Os01g0894300	Q0JGZ6	Fructokinase-1	0.53 ± 0.04	ns	ns
46	Os01g0841600	Q8LR75	Triosephosphate isomerase	ns	0.33 ± 0.05	ns
49	Os06g0675700	Q0DA62	Probable alpha-glucosidase Os06g0675700	4.71 ± 1.86	2.51 ± 0.76	ns
84	Os09g0553200	Q93X08	Os09g0553200 protein	3.23 ± 0.63	ns	ns
86	Os05g0482700	Q5KQH5	Putative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase	0.60 ± 0.05	ns	ns
94	Os08g0520900	Q0J4C6	Os08g0520900 protein	3.19 ± 0.38	2.50 ± 0.36	ns
118	Os08g0191433	Q6Z1D6	Putative starch synthase DULL1	2.45 ± 0.16	ns	2.43 ± 0.19
132	Os04g0164900	Q7X834	OSJNBa0019G23.2 protein/pullulanase	20.08 ± 1.58	5.66 ± 0.45	3.84 ± 0.56
152	Os04g0526600	Q0JBL0	Alpha-amylase/subtilisin inhibitor	6.59 ± 0.45	3.02 ± 0.06	ns
158	Os02g0528200	Q6H6P8	Branching enzyme-3/SBE3	2.36 ± 0.36	ns	ns
159	Os06g0726400	Q0D9D0	Os06g0726400 protein/SBE1	15.54 ± 0.72	3.34 ± 0.24	5.00 ± 0.42
Respration (Glycolysis,TCA and Fermentation)
4	Os01g0905800	Q5N725	Fructose-bisphosphate aldolase	3.99 ± 0.62	ns	ns
27	Os01g0926300	Q5JK10	Os01g0926300 protein	0.37 ± 0.07	ns	ns
30	Os02g0601300	Q6K5G8	Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic	3.76 ± 0.30	ns	ns
59	Os02g0169300	Q6H6C7	Phosphoglycerate kinase	4.84 ± 0.51	ns	ns
69	Os06g0668200	Q655T1	Phosphoglycerate kinase	2.56 ± 0.42	ns	ns
76	Os10g0478200	Q7XDC8	Malate dehydrogenase, cytoplasmic	3.51 ± 0.12	ns	ns
82	Os08g0191700	Q0J7H9	Lactoylglutathione lyase	3.30 ± 0.40	3.14 ± 1.07	ns
89	Os11g0210500	Q0ITW7	Alcohol dehydrogenase 2	5.38 ± 1.61	ns	ns
104	Os06g0486800	Q0DC43	Formate dehydrogenase	3.25 ± 0.54	2.41 ± 0.74	ns
120	Os08g0545200	Q6ZBH2	Os08g0545200 protein/Sorbitol dehydrogenase	12.37 ± 2.25	2.49 ± 0.24	5.09 ± 0.97
122	Os03g0293500	Q10MW3	Pyruvate decarboxylase 2	3.96 ± 0.28	2.72 ± 1.11	ns
125	Os06g0326400	Q69T78	Pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit alpha	2.05 ± 0.21	ns	2.27 ± 0.28
127	Os07g0187200	Q7XI14	Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial	4.60 ± 0.81	ns	ns
173	Os04g0486950	Q7XUG1	Malate synthase	5.85 ± 1.70	4.64 ± 0.51	ns
Photosynthesis
1	Os01g0711000	Q8S7T5	ATP synthase subunit alpha	ns	4.23 ± 1.46	ns
3	Os10g0356000	P0C512	Ribulose bisphosphate carboxylase large chain	2.74 ± 0.09	2.40 ± 0.75	ns
7	Q8S6G5[a]	Q8S6G5	Photosystem II CP43 reaction center protein	ns	1.92 ± 0.09	ns
23	Q69VC8[b]	Q69VC8	Photosystem II CP47 reaction center protein	ns	3.17 ± 1.27	ns
43	Os03g0563300	Q53RM0	Magnesium-chelatase subunit ChlI, chloroplastic	0.46 ± 0.01	ns	ns
57	Os10g0492000	Q9FWV2	Putative chloroplast inner envelope protein	2.36 ± 0.57	ns	ns
Material transport
22	Os07g0448800	Q8H5N9	Probable aquaporin PIP2–1	ns	3.21 ± 0.71	ns
64	Os08g0513600	Q6Z8M9	Os08g0513600 protein	6.26 ± 2.17	ns	ns
75	Os02g0202400	Q6Z782	Os02g0202400 protein	3.04 ± 0.84	ns	ns
78	Os11g0644100	Q2R0I6	Leucine Rich Repeat family protein, expressed	0.24 ± 0.02	ns	ns
111	Os03g0271200	Q10NF2	Protein TOC75, chloroplastic	4.63 ± 0.36	1.95 ± 0.32	2.45 ± 0.41
123	Os05g0111200	Q65XV6	Os05g0111200 protein	3.77 ± 0.18	2.85 ± 0.23	ns
126	Os03g0240500	Q10PB3	Translocase of chloroplast	2.27 ± 0.17	ns	ns
Signal transduction
2	Os03g0710800	Q10E23	14–3-3-like protein GF14-F	0.53 ± 0.01	ns	ns
101	Os06g0110100	Q8H684	OSEYA1	4.56 ± 1.10	1.86 ± 0.18	2.51 ± 0.58
129	Os01g0356800	Q0JMV9	Os01g0356800 protein/ GTP binding protein	35.53 ± 10.52	2.45 ± 0.06	12.89 ± 1.52
138	Os02g0799000	Q69QZ0	Probable protein phosphatase 2C 27	5.05 ± 0.74	ns	ns
Stress and defense
5	Os07g0186000	Q0D840	Thioredoxin H1	ns	3.65 ± 0.82	ns
8	Os02g0115700	Q0E4K1	Catalase isozyme A	0.28 ± 0.01	ns	0.49 ± 0.07
10	Os05g0116100	Q65XA0	Dehydroascorbate reductase	2.15 ± 0.13	2.97 ± 0.40	ns
11	Os05g0323900	Q43008	Superoxide dismutase [Mn], mitochondrial	1.77 ± 0.05	3.29 ± 0.33	ns
21	Os04g0508300	P55142	Glutaredoxin-C6	2.95 ± 0.11	3.44 ± 0.66	ns
24	Os05g0157200	Q75M01	Os05g0157200 protein	ns	2.97 ± 0.28	ns
35	Os12g0244100	Q2QV45	70 kDa heat shock protein	5.12 ± 1.21	2.18 ± 0.62	ns
58	Os05g0453700	Q7XXS5	Os05g0453700 protein	4.37 ± 0.28	3.23 ± 0.16	ns
67	Os07g0624600	Q7XI41	Probable trehalose-phosphate phosphatase 3	0.35 ± 0.03	0.29 ± 0.08	ns
73	Os12g0514500	Q0IN14	Hsp90 protein, expressed	0.36 ± 0.05	0.32 ± 0.05	ns
81	Os01g0270100	Q0JNR2	Cysteine proteinase inhibitor 12	2.14 ± 0.21	ns	ns
113	Os07g0694700	Q0D3B8	Ascorbate peroxidase	0.51 ± 0.06	ns	ns
134	Os01g0663400	Q0JKM8	Os01g0663400 protein	8.82 ± 0.50	2.32 ± 0.43	3.68 ± 0.78
166	Os07g0638300	P0C5C9	1-Cys peroxiredoxin A	14.91 ± 5.52	3.85 ± 0.39	3.59 ± 1.15
174	Os03g0245800	Q10P60	26.7 kDa heat shock protein, chloroplastic	4.84 ± 0.88	2.16 ± 0.53	ns
Protein synthesis and destination
12	Os04g0685200	Q7XPU2	OSJNBa0088H09.14 protein	0.38 ± 0.04	ns	ns
16	Os06g0687700	Q653F6	Putative t-complex protein 1 theta chain	0.40 ± 0.01	ns	ns
25	Os12g0277500	Q2QU06	60 kDa chaperonin alpha subunit	0.21 ± 0.03	0.23 ± 0.05	ns
34	Os03g0804800	Q75HJ3	Putative TCP-1/cpn60 chaperonin family protein	0.50 ± 0.06	0.61 ± 0.10	ns
48	Os02g0332200	Q6YUK5	T-complex protein 1 subunit delta	0.27 ± 0.03	0.32 ± 0.05	ns
68	Os07g0578300	Q6ZL89	Os07g0578300 protein	0.05 ± 0.01	0.09 ± 0.03	0.38 ± 0.01
71	Os03g0619400	Q6AV23	Putative TCP-1/cpn60 chaperonin family protein	0.40 ± 0.05	ns	ns
79	Os02g0717400	Q6ZGV8	Clustered mitochondria protein homolog	ns	0.30 ± 0.08	ns
95	Os03g0565500	Q10I39	Elongation factor G, mitochondrial, putative, expressed	0.32 ± 0.08	0.33 ± 0.08	ns
103	Os09g0491772	B9G4B3	Os09g0491772 protein	0.16 ± 0.04	0.15 ± 0.04	ns
110	Os02g0100100	Q67IX6	Protein disulfide isomerase-like 1–4	5.62 ± 0.64	ns	3.37 ± 0.67
117	Os01g0185200	Q5VRX8	Os01g0185200 protein	3.21 ± 0.26	ns	ns
119	Os01g0752700	Q5JMX4	Os01g0752700 protein	0.31 ± 0.04	0.28 ± 0.02	ns
130	Os09g0451500	Q67UF5	Protein disulfide isomerase-like 2–3	2.15 ± 0.47	ns	ns
135	Os02g0506500	Q6K6K7	Ubiquitin-like modifier-activating enzyme 5	3.28 ± 1.07	ns	ns
143	Os09g0252800	Q6K3Y7	Putative ubiquitin-protein ligase 1	ns	0.53 ± 0.04	ns
144	Os02g0115900	Q6Z7B0	Dnak-type molecular chaperone Bip	6.30 ± 1.31	ns	3.84 ± 1.04
147	Os05g0557200	Q6I605	Os05g0557200 protein	2.13 ± 0.15	ns	ns
148	Os07g0215500	Q0D7S0	Allergen RA5B	22.26 ± 5.08	4.59 ± 0.37	ns
153	Os07g0213800	Q8H4M4	Allergenic protein	ns	5.68 ± 0.20	ns
155	Os11g0199200	Q53LQ0	Protein disulfide isomerase-like 1–1	4.62 ± 0.40	ns	ns
161	Os03g0610650	Q75H81	Serpin-ZXA	3.53 ± 0.52	2.30 ± 0.33	ns
170	Os05g0519700	Q6F2Y7	Chaperone protein ClpB1	4.54 ± 0.18	2.35 ± 0.39	1.98 ± 0.35
Storage proteins
97	Os07g0609000	Q6YTX6	Seed protein	19.17 ± 2.41	6.65 ± 2.22	ns
99	Os01g0762500	Q0JJ36	Glutelin	1.54 ± 0.02	ns	ns
128	Os03g0793700	Q852L2	Cupin family protein, expressed	8.40 ± 1.03	4.63 ± 0.42	ns
141	Os05g0499100	Q0DH05	Alpha-globulin	39.03 ± 4.70	4.02 ± 0.47	ns
145	Os02g0242600	Q6ESW6	Glutelin	19.54 ± 5.20	ns	9.51 ± 1.33
151	Os03g0197300	Q0DUA3	Os03g0197300 protein	25.94 ± 10.52	6.59 ± 1.98	ns
157	Os02g0456100	Q6K7K6	Glutelin	36.64 ± 4.06	3.66 ± 0.31	11.87 ± 0.71
163	Os03g0336100	Q0DS36	Os03g0336100 protein	23.65 ± 1.52	3.60 ± 0.61	ns
165	Os02g0249900	Q0E2D2	Glutelin	ns	4.24 ± 0.89	ns
167	Os07g0214300	Q0D7S4	Seed allergenic protein RAG2	12.44 ± 2.51	6.26 ± 0.42	ns
169	Os02g0249000	Q6K508	Glutelin	28.90 ± 5.78	1.86 ± 0.30	14.62 ± 1.20
171	Os03g0663800	Q75GX9	Cupin family protein, expressed	31.11 ± 3.49	ns	ns
172	Os02g0268300	Q0E261	Glutelin	56.76 ± 1.25	4.28 ± 0.71	ns
Amino acid metabolism
9	Os08g0447000	Q6ZAA5	D-3-phosphoglycerate dehydrogenase	0.18 ± 0.06	0.28 ± 0.11	ns
20	Os11g0216900	Q0ITU1	Methylthioribose-1-phosphate isomerase	0.31 ± 0.01	ns	ns
52	Os03g0738400	Q7Y1F0	Serine hydroxymethyltransferase	1.99 ± 0.05	ns	ns
66	Os03g0223400	Q10PS4	Glutamine synthetase	5.20 ± 0.10	3.93 ± 1.24	ns
70	Os09g0255400	Q8H3R5	Putative indole-3-glycerol phosphate synthase	3.82 ± 0.69	ns	ns
85	Os12g0235800	Q2QVC1	Argininosuccinate synthase, chloroplast, putative, expressed	2.74 ± 0.13	ns	ns
92	Os03g0136200	Q10S41	Methyltransferase	0.42 ± 0.04	ns	ns
93	Os12g0607000	Q2QME6	Homocysteine S-methyltransferase 3	3.83 ± 0.20	ns	ns
105	Os12g0138900	Q2QXY9	2-isopropylmalate synthase B, putative, expressed	2.75 ± 0.17	1.81 ± 0.27	ns
114	Os02g0783625	Q6K7D6	Putative lysine-ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme	3.13 ± 0.56	ns	ns
140	Os12g0145100	Q2QXS4	Os12g0145100 protein	ns	ns	2.63 ± 0.68
142	Os10g0390500	Q94HC5	Putative alanine amino transferase	3.07 ± 0.38	ns	3.02 ± 0.44
149	Os12g0578200	Q2QN58	Chorismate mutase, chloroplast, putative, expressed	18.67 ± 2.19	1.92 ± 0.11	8.26 ± 0.90
150	Os03g0171900	Q10R45	Alanine-glyoxylate aminotransferase 2, mitochondrial, putative, expressed	3.68 ± 0.78	ns	ns
160	Os04g0389800	Q0E0Z3	Acetolactate synthase	3.41 ± 0.30	1.80 ± 0.24	ns
162	Os01g0760600	Q0JJ47	Aspartate aminotransferase	3.82 ± 0.36	ns	3.30 ± 0.31
Nucleotides
29	Os10g0539500	Q7XUC9	Histone H4	0.39 ± 0.03	ns	ns
37	Os01g0550000	Q5JK84	DEAD-box ATP-dependent RNA helicase 15	0.54 ± 0.06	ns	ns
60	Os01g0275600	Q9SDG8	Protein argonaute 4A	0.32 ± 0.06	0.43 ± 0.09	ns
63	Os02g0736400	Q6Z744	Dihydropyrimidine dehydrogenase	2.25 ± 0.17	ns	ns
87	Os03g0158500	Q8H8C1	Putative RNA-binding protein	ns	0.31 ± 0.05	ns
88	Os02g0214500	Q6H8A9	NAC6	4.80 ± 0.64	ns	ns
102	Os07g0471300	Q69UP6	Protein argonaute 18	0.37 ± 0.08	0.41 ± 0.09	ns
107	Os02g0137400	Q6YXY3	Putative splicing factor 3b, subunit 3, 130 kDa	0.56 ± 0.03	ns	ns
109	Os02g0821800	Q6AT27	Putative fibrillarin	0.42 ± 0.08	ns	ns
115	Os07g0212300	Q8H4U7	Mut T-like protein	10.59 ± 1.42	ns	ns
131	Os02g0523500	Q6H547	Os02g0523500 protein	2.18 ± 0.24	ns	1.95 ± 0.34
Lipid metabolism
28	Os05g0295300	B9FK36	Acetyl-CoA carboxylase 2	0.64 ± 0.01	ns	ns
51	Os11g0558300	Q2R2L5	AMP-binding enzyme family protein, expressed	0.34 ± 0.14	ns	ns
56	Os03g0181500	Q8H7L2	3-ketoacyl-CoA synthase	0.17 ± 0.02	0.25 ± 0.01	ns
62	Os06g0156700	Q5VMA4	Os06g0156700 protein	3.49 ± 0.43	ns	ns
77	Os05g0567100	Q0DFW1	Aspartic proteinase oryzasin 1	2.58 ± 0.49	2.54 ± 0.40	ns
96	Os01g0880800	Q8LJJ9	Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic	ns	0.35 ± 0.05	ns
108	Os07g0188800	Q6Z4E4	Methylmalonate semi-aldehyde dehydrogenase	2.13 ± 0.19	2.04 ± 0.40	ns
124	Os06g0260500	Q5Z7E7	3-ketoacyl-CoA synthase	0.24 ± 0.10	0.26 ± 0.12	ns
146	Os01g0348600	Q94CN1	Os01g0348600 protein	0.38 ± 0.15	ns	ns
Secondary metabolism
31	Os07g0529600	Q7XXS4	Thiamine biosynthetic enzyme	0.30 ± 0.02	0.30 ± 0.02	ns
47	Os08g0157500	Q6ZD89	Flavone 3′-O-methyltransferase 1	4.73 ± 0.80	2.59 ± 0.69	ns
55	Os08g0498400	Q7F8T6	Tricin synthase 2	3.56 ± 0.56	2.19 ± 0.30	ns
116	Os09g0446800	Q0J1E1	Os09g0446800 protein	2.55 ± 0.03	1.67 ± 0.18	ns
139	Os03g0192700	Q10QK8	Inositol-3-phosphate synthase	2.06 ± 0.18	ns	ns
164	Os08g0189100	Q6YZA9	Germin-like protein 8–2	7.61 ± 0.71	5.34 ± 0.86	ns
Unknown
13	Os12g0555500	Q2QNS7	Os12g0555500 protein	0.49 ± 0.03	ns	ns
15	Os06g0646500	Q67W57	Os06g0646500 protein	ns	ns	0.57 ± 0.04
17	Os03g0278200	Q10N92	Os03g0278200 protein	0.46 ± 0.05	ns	ns
18	Os11g0687100	Q2QZH3	Os11g0687100 protein	0.08 ± 0.03	0.21 ± 0.11	0.31 ± 0.05
32	Os12g0182200	Q2QWU7	Dihydrolipoamide S-acetyltransferase, putative, expressed	0.53 ± 0.05	ns	ns
39	Os06g0613000	Q69WY2	Uncharacterized protein	0.36 ± 0.04	ns	ns
53	Os01g0916600	Q7F2X8	Os01g0916600 protein/OsGRP2	0.24 ± 0.01	0.28 ± 0.01	ns
61	Os11g0687200	Q2QZH2	Expressed protein	0.30 ± 0.05	ns	0.54 ± 0.03
72	Os07g0409100	Q7XTM4	OSJNBa0033G05.21 protein	0.53 ± 0.05	ns	ns
74	Os07g0638100	Q8GVH2	Os07g0638100 protein	0.53 ± 0.03	ns	ns
80	Os01g0128400	Q9LGA3	Os01g0128400 protein	2.00 ± 0.06	ns	ns
83	Os04g0531900	Q7X8W6	OSJNBa0081C01.20 protein	5.73 ± 1.54	4.34 ± 0.33	ns
90	Os10g0463200	Q8H906	Putative early nodulin gene (Enod) related protein	0.36 ± 0.03	0.46 ± 0.04	ns
98	Os03g0327600	Q10M12	Expressed protein	2.03 ± 0.08	ns	ns
100	Os07g0568700	Q0D5C7	Os07g0568700 protein	0.44 ± 0.03	ns	ns
106	Os04g0482800	Q7XUP3	OSJNBb0011N17.20 protein	3.73 ± 0.15	2.14 ± 0.10	ns
112	Os02g0783700	Q0DX00	Os02g0783700 protein	2.05 ± 0.19	ns	ns
133	Os05g0132100	Q0DL03	Os05g0132100 protein	0.34 ± 0.05	ns	ns
137	Os06g0214300	Q69Y21	Os06g0214300 protein	2.85 ± 0.43	1.68 ± 0.16	ns
156	Os04g0404400	Q7X6I8	OJ000315_02.8 protein	16.73 ± 7.88	5.11 ± 1.18	ns
168	Os10g0463800	Q337M4	Os10g0463800 protein	3.60 ± 0.15	2.59 ± 0.29	ns

Accession: the code of the identified protein in RAP database (http://rapdb.dna.affrc.go.jp/); [a] and [b]: the code of these two identified proteins in RAP database were not found; ns means no significant change of protein abundance between the two compared samples; T0: control treatment with no spikelet thinning; T2: the upper 2/3 of spikelets were removed. SS: Superior spikelets; IS: Inferior spikelets; Values are means ± S.D. of two replications. The screening criteria for differentially expressed proteins was a fold change >1.5 or <0.67 and a p-value <0.05

Back to article page

ISSN: 1471-2229

Contact us

General enquiries: journalsubmissions@springernature.com

BMC Plant Biology

Contact us