- Research article
- Open Access
Prediction and analysis of three gene families related to leaf rust (Puccinia triticina) resistance in wheat (Triticum aestivum L.)
BMC Plant Biology volume 17, Article number: 108 (2017)
The resistance to leaf rust (Lr) caused by Puccinia triticina in wheat (Triticum aestivum L.) has been well studied over the past decades with over 70 Lr genes being mapped on different chromosomes and numerous QTLs (quantitative trait loci) being detected or mapped using DNA markers. Such resistance is often divided into race-specific and race-nonspecific resistance. The race-nonspecific resistance can be further divided into resistance to most or all races of the same pathogen and resistance to multiple pathogens. At the molecular level, these three types of resistance may cover across the whole spectrum of pathogen specificities that are controlled by genes encoding different protein families in wheat. The objective of this study is to predict and analyze genes in three such families: NBS-LRR (nucleotide-binding sites and leucine-rich repeats or NLR), START (Steroidogenic Acute Regulatory protein [STaR] related lipid-transfer) and ABC (ATP-Binding Cassette) transporter. The focus of the analysis is on the patterns of relationships between these protein-coding genes within the gene families and QTLs detected for leaf rust resistance.
We predicted 526 ABC, 1117 NLR and 144 START genes in the hexaploid wheat genome through a domain analysis of wheat proteome. Of the 1809 SNPs from leaf rust resistance QTLs in seedling and adult stages of wheat, 126 SNPs were found within coding regions of these genes or their neighborhood (5 Kb upstream from transcription start site [TSS] or downstream from transcription termination site [TTS] of the genes). Forty-three of these SNPs for adult resistance and 18 SNPs for seedling resistance reside within coding or neighboring regions of the ABC genes whereas 14 SNPs for adult resistance and 29 SNPs for seedling resistance reside within coding or neighboring regions of the NLR gene. Moreover, we found 17 nonsynonymous SNPs for adult resistance and five SNPs for seedling resistance in the ABC genes, and five nonsynonymous SNPs for adult resistance and six SNPs for seedling resistance in the NLR genes. Most of these coding SNPs were predicted to alter encoded amino acids and such information may serve as a starting point towards more thorough molecular and functional characterization of the designated Lr genes. Using the primer sequences of 99 known non-SNP markers from leaf rust resistance QTLs, we found candidate genes closely linked to these markers, including Lr34 with distances to its two gene-specific markers being 1212 bases (to cssfr1) and 2189 bases (to cssfr2).
This study represents a comprehensive analysis of ABC, NLR and START genes in the hexaploid wheat genome and their physical relationships with QTLs for leaf rust resistance at seedling and adult stages. Our analysis suggests that the ABC (and START) genes are more likely to be co-located with QTLs for race-nonspecific, adult resistance whereas the NLR genes are more likely to be co-located with QTLs for race-specific resistance that would be often expressed at the seedling stage. Though our analysis was hampered by inaccurate or unknown physical positions of numerous QTLs due to the incomplete assembly of the complex hexaploid wheat genome that is currently available, the observed associations between (i) QTLs for race-specific resistance and NLR genes and (ii) QTLs for nonspecific resistance and ABC genes will help discover SNP variants for leaf rust resistance at seedling and adult stages. The genes containing nonsynonymous SNPs are promising candidates that can be investigated in future studies as potential new sources of leaf rust resistance in wheat breeding.
Leaf rust is a fungal disease caused by Puccinia triticina (= P. recondita Roberge ex Desmaz. f. sp. tritici.) which has been a serious threat to the world production of bread wheat (Triticum aestivum L.) and other cereals over the past decades [1,2,3]. The leaf rust pathogen is a biotrophic parasite with many physiological races that often are highly specific to wheat cultivars with compatible resistance genes. To date, at least 75 leaf rust (Lr) resistance genes have been identified, and the majority of them confer the race-specific resistance in the seedling stage . However, a few race-nonspecific Lr genes including Lr34 and Lr67 have been also found particularly at adult stage, conferring resistance to multiple pathogen species [5,6,7,8,9].
According to Krattinger et al. , the Lr genes in wheat and other cereals may be divided into three groups based on their specificity and durability. The first group contains genes that confer race-specific resistance against one but not other races of the same pathogen species. As mentioned above, the majority of the Lr genes are within this group. These Lr genes often encode intracellular immune receptor proteins with nucleotide-binding sites and leucine-rich repeats (NLR, also known as NBS-LRR or R genes). Proteins encoded by R genes directly or indirectly perceive pathogen-derived virulence effectors that are secreted into the cytoplasm of host cells in order to suppress basal immunity. The second group contains genes that confer race-nonspecific resistance against multiple fungal pathogens simultaneously. The well-studied example in this group is Lr34. This locus was first reported by Dyck  and it was mapped on wheat chromosome 7D. It has been subsequently known to confer resistance against multiple diseases including leaf rust with the resistance gene named as Lr34, stem rust (caused by P. graminis) with the resistance gene named as Sr57, stripe (yellow) rust (caused by P. striiformis) with the resistance gene named as Yr18, powdery mildew (caused by Blumeria graminis) with the resistance gene named as Pm38 and barley yellow dwarf virus with the resistance gene named as Bdv1 [11,12,13,14]. Thus, Lr34 has other designations including Lr34/Yr18, Lr34/Yr18/Sr57/Pm38 and Lr34/Yr18/Sr57/Pm38/Bdv1 in the literature. Lr34 has been molecularly characterized and it encodes a putative ABC transporter containing transmembrane (TM) and nucleotide binding (NB) domains [6, 15]. The third group, like the second group, confers race-nonspecific resistance, but unlike the second group, such resistance is against all races within the same pathogen species. A known example in this group is a gene with resistance to stripe (yellow) rust in wheat (Yr36), and the resistance genes in the group are called START genes because they code for a START (steroidogenic acute regulatory [StAR] protein-related lipid transfer domain) protein . Additional examples of START genes in other cereal species include the recessive rice blast resistance gene pi21 encoding a small proline-rich protein , and the recessive barley powdery mildew resistance gene mlo coding for a membrane-anchored protein .
A common feature among the NLR, ABC and START proteins described above is the presence of distinct domains within each family. In the NLR group, a large number of potential R-genes or resistance gene analog (RGAs) encode R-proteins or effector-recognition receptors known as intracellular immune receptors and most belong to nucleotide-binding site-LRR (NBS-LRR or NLR) class  including seven domains or motifs: Toll/Interleukin-1 receptor (TIR-NBS-LRR or TNL), coiled-coil (CC- NBS-LRR or CNL), leucine zipper (LZ), NBS, LRR, TM and serinethreonine kinase (STK) . In the ABC group, the only well-characterized gene (Lr34) encodes a full-size ATP-binding cassette (ABC) transporter and this protein is a member of the ABCG subfamily which is also known as the PDR (pleiotropic drug resistance) subfamily [6, 10, 12] and this protein impedes the invasion and spread of compatible pathogens in wheat and other cereals . The functions of Lr34 are constitutive rather than induced because the gene functions irrespective of whether or not pathogens are present. There is no known Lr gene that is yet available in the START group. Yr36 is the only rust resistance gene in the group that is currently known to confer resistance to a broad spectrum of stripe rust races in wheat and this adult resistance is highly expressed at high temperatures (25-35 °C) . In general, the START genes are not well studied but they are known to encode proteins for many functions in plants. The first function of the START proteins is, of course, the resistance to plant pathogens (e.g., the three START genes described above: Yr36, pi21  and mlo ). The second function is the response to abiotic stresses, e.g., increased expression of transmembrane START (TM-START) genes in chickpea in response to drought, salt, wound and heat stresses . The third function is the ability to modulate transcription factor activity in Arabidopsis .
RNA-Seq is a more accurate method of quantifying gene expression levels than previous expression assay techniques such as microarray . As RNA-Seq works without need for a genome sequence, it enables joint assays of host and pathogen transcriptomes, thereby gaining insights into how pathogens regulate the expression of their genes for disease progression and how they influence the host plant’s circuitry during a defense response [25,26,27]. A recent study  reported a detailed RNA-Seq time-course for a susceptible wheat cultivar (Vuka) and a resistant line (Avocet-Yr5) inoculated with the wheat yellow rust pathogen Puccinia striiformis f. sp. tritici (Pst) at different days post-inoculation (dpi). These authors were able to identify clusters of differentially expressed genes in wheat plants and Pst. For example, they identified a total of seven clusters of genes with similar expression profiles that were enriched in GO (gene ontology) term annotations and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway memberships for the wheat host. In particular, their Cluster III of host genes had a peak expression at 11 dpi and those genes for membrane transport and ABC transporters and chitinases in this cluster were significantly enriched. Thus, it would be desired to identify the expression profiles of the host genes that belong to the ABC, NLR and START gene families.
Recent QTL mapping studies have reported several hundred QTLs for rust resistance in wheat populations [28,29,30,31]. Additionally, novel QTLs associated with wheat rust resistance have been reported in several genome-wide association studies (GWAS) [32,33,34], using the 9 K or 90 K single nucleotide polymorphism (SNP) chips [35, 36]. Most of the QTLs for leaf rust resistance at the seedling and adult stages are now stored in the T3 database . However, little is known about the physical relationships between these QTLs and the ABC, NLR and START genes. For example, it may be expected  that the ABC (and START) genes are more likely to share genomic regions with QTLs for race-nonspecific adult resistance whereas the NLR genes are more likely to share genomic regions with QTLs for race-specific resistance that is readily expressed in the seedlings. The objective of this study is to conduct bioinformatic prediction and gene annotation within ABC, NLR and START gene families in wheat that share genomic regions with QTLs for leaf rust resistance at the seedling and adult stages as obtained from the T3 database . Specifically, we first predicted the putative ABC, NLR and START genes across the wheat genome. Then we attempted to establish physical relationships between these putative genes and the designated Lr genes from the sequences of SNP markers flanking the leaf rust QTLs. Together, this work provides an important framework for future studies to discover molecular functions of existing and new rust resistance genes in the wheat gene pool for their deployment in the development of wheat cultivars with improved rust resistance.
Putative ABC, NLR and START proteins in wheat
In total, we predicted 526 ABC genes in the wheat genome. These ABC proteins were classified into eight subfamilies with the subfamily G further divided into Gwbc and Gpdr (Table 1). Our results show that the G (Gwbc and Gpdr), C, B and I subfamilies in wheat are the four largest in the ABC family, accounting for 30.4%, 23.0%, 18.8% and 16.2% of total 526 ABC genes, respectively. Notably, 78 ABC Gpdr transporters were predicted in the wheat genome, slightly more than a previous estimate of 60 full-size ABCG transporters estimated from the full-size ABCG gene numbers in Arabidopsis and rice [15, 38]. In comparison, we also performed a parallel analysis in Arabidopsis to validate the prediction pipeline. Our result showed that, of 131 ABC proteins found in Arabidopsis G (33.5%), B (20.8%), I (16.1%), C (12.8%) were the four largest subfamilies, which is consistent with the Arabidopsis result on the ABC proteins recently reported by Andolfo et al. . These authors also reported 146 ABC proteins in rice , including 7 (4.8%), 29 (19.9%), 18 (12.3%), 3 (2.1%), 3 (2.1%), 7 (4.8%), 41 (28.1%), 22 (15.1%), and 16 (11.0%) in A, B, C, D, E, F, Gwbc, Gpdr and I families, respectively.
For the NLR family, we predicted 1117 NLR proteins in wheat (Table 1). This number is slightly higher than those in previous studies of the genome-wide analysis of NLR-coding genes in wheat [40, 41] but slightly lower than the 1185 NLR proteins predicted using the new gene models in the wheat genome assembly recently generated by Earlham Institute . In a concurrent analysis, we predicted 249 NLR proteins in Arabidopsis and the majority of them were TNL (Tir-NBS-LRR), accounting for nearly 69% (171), compared to less than 21% (52) of CNL (Coiled-coil-NBS-LRR) proteins. This high TNL/CNL ratio was consistent with previous studies of the NLR proteins in dicot species [40, 43, 44]. In contrast, nearly 88% (979) of the wheat NLR proteins were classified to the CNL class. However, nine (0.8%) wheat TNL proteins were detected using NLR-Parser, contrary to the commonly accepted belief that TNL genes might have been lost completely in monocots [19, 40, 45]. These nine proteins were further analyzed with PfamScan  and the analysis indicated that none of them contains a predicted TIR domain (PF01582), The analysis also showed that eight of them contain a truncated NB-ARC domain (PF00931) whose length ranges from 63 to 271 amino acids (the full length of the NB-ARC domain is 288 in Pfam) and the remaining one (Traes_3B_706A56165) was annotated by PfamScan as a Ceramidase family protein. Thus, this limited number of TNL proteins predicted in wheat is likely due to NLR-Parser annotation error arising from the presence of truncated NB-ARC domains. In addition, 10.4% in Arabidopsis and 11.6% in wheat of the NLR proteins could not be classified to either CNL or TNL, which were designated as ‘N/A’ by NLR-Parser.
The START family genes were predicted exclusively based on the presence of START (IPR002913 and IPR005031) and/or START-like domain (IPR023393) following domain analysis of the wheat proteome using InterProscan . From this analysis, we detected 139 and 144 START genes in Arabidopsis and wheat, respectively. Among the five classes, the START class was the largest in both Arabidopsis and wheat, with 75 (54%) in the former and 54 (37.5%) in the latter. HD-START was the second largest class in Arabidopsis with 21 members (15.1%), whereas MINIMAL-START was the second largest class in wheat with 47 members (32.6%). In both species, HD-START-MEKHLA was the smallest, each with seven members accounting for 5.0% in Arabidopsis and 4.9% in wheat. In an early study, only 35 Arabidopsis START genes were reported , but these authors used only five START-domain proteins (Arabidopsis GL2 and ATML1, rice ROC1 and AAP54082, and human PCTP) as queries for BLAST searches. Recently, Satheeth and colleagues  reported 36 START genes in chickpea (Cicer arietinum L.), using the START domain (~200 amino acids) encoded by the Arabidopsis gene AT5G54170 (in our Arabidopsis START gene list) as query sequence in the TBLASTN search against the genome contigs database of chickpea. To the best of our knowledge, no genome-scale analysis of START proteins has been reported in wheat.
Our prediction was primarily based on domain analysis, with their domains summarized in Additional file 1. However, our classification of the putative ABC proteins in wheat into different subfamilies was based on protein sequence homology to the ABCs in Arabidopsis and rice (Oryza sativa). Therefore, we analyzed sequence similarity of ABC proteins and their two main types of domains (transmembrane domains or TMDs, and cytosolic nucleotide binding domains or NBDs) between wheat, Arabidopsis and rice (Fig. 1). The protein sequence identity was higher between wheat and rice than between wheat and Arabidopsis. On average, these entire ABC proteins shared 62.2% sequence identity (S.D. ± 14.9%) between wheat and Arabidopsis, and 71.3% (S.D. ± 19.1%) between wheat and rice (Fig. 1a). As expected, the ABC domains showed greater homology in both wheat-Arabidopsis and wheat-rice comparisons, especially for the NBDs including ABC transporter-like domain (IPR003439), AAA+ ATPase domain (IPR003593) and P-loop NTPase fold (IPR027417). For instance, the ABC transporter type 1 transmembrane domain (IPR011527) had an average sequence identity of 65.1% between wheat and Arabidopsis, and 85.2% between wheat and rice (Fig. 1b). Likewise, the average identity of the ABC-2 transporter domain (IPR013525) was 64.8% between wheat and Arabidopsis, and 85.1% between wheat and rice (Fig. 1c). For the ABC transporter-like domain (IPR003439) the average identity increased to 77.4% between wheat and Arabidopsis, and 90.0% between wheat and rice (Fig. 1d). The homology of the AAA+ ATPase domain (IPR003593) was even higher between these species, with the average identity reaching 79.1% between wheat and Arabidopsis and 90.7% between wheat and rice (Fig. 1e). Lastly, the P-loop NTPase fold (IPR027417) showed slightly lower homology, with an average idenitity of 75.8% between wheat and Arabidopsis and 89.1% between wheat and rice (Fig. 1f). Although the sequence identity between wheat and rice ABC proteins is mostly higher than between wheat and Arabidopsis, the top hit of some wheat ABC proteins was from Arabidopsis. Thus, we used both Arabidopsis and rice ABCs to classfiy the wheat ABC proteins.
Annotation of the wheat ABC, NLR and START genes
As almost all ABC, NLR and START genes have no informative annotation (only ambiguous annotation such as uncharacterized protein or hypothetical protein) in the Ensembl Plants database , we enhanced their annotation using sequence analysis with BLAST . We first performed BLAST searches against the known plant disease resistance genes in GenBank and PRGdb [51, 52]. Though 145 ABC proteins showed various degrees of sequence similarity to the known LR34 protein, only two had more than 97% identity (Additional file 2), suggesting that the respective Lr34 and Lr34-B genes are Traes_7DS_25B02BA46 and Traes_4AL_603B6DC64 in Ensembl. Traes_7DS_25B02BA46 is partial, encoding only 799 amino acids (aa) instead of 1401 aa for the full-length LR34 (Additional file 2). In the Ensembl release 33, we found the full-length Lr34 gene (TRIAE_CS42_7DS_TGACv1_621754_AA2025300) encoding a protein of 1401 aa. Our annotation of Lr34 and Lr34-B was confirmed by multiple sequence alignment (MSA) of the LR34 and LR34-B proteins in Ensembl as well as the known resistant (accession: ACN41354) and susceptible (ADK62371) LR34 proteins in GenBank (Fig. 2). Interestingly, this MSA indicated that the putative LR34 proteins in Ensembl (both Traes_7DS_25B02BA46 in release 31 and TRIAE_CS42_7DS_TGACv1_621754_AA2025300 in release 33) represent the resistant version of LR34. In addition, the putative LR34-B (Traes_4AL_603B6DC64) protein has about 97% sequence identity with LR34 (Additional file 2), consistent with a previous study . In contrast, in the NLR and START families, no genes showed over 90% sequence identity with known rust related proteins, and thus unlikely to represent the same genes. For example, Traes_1DS_5FF8D9E2D in the NLR family exhibited an identity of ~85% with the known stripe rust resistance protein YR10 in GenBank (accession: AAG42168). In the START family, only the sequence of a kinase-START domain protein from T. dicoccoides was found in GenBank (ACF33195), and the highest identity was ~63% at the protein sequence level with Traes_7DL_A330C0F90 (Additional file 2). The relative low sequence identity may be attributed to (i) the use of Chinese Spring (CS) for genome sequencing and different wheat cultivars for gene isolation and (ii) increased allelic diversity of R genes among the wheat accessions. For example, Lr1 was cloned from Glenlea  and Lr10 from ThatcherLr10 . In contrast, it is reasonable to expect that when the same wheat genotype is used for both genome sequencing and gene cloning perfect sequence matching may be found. The only example we have found so far for this case is Lr34 whose sequencing and cloning was based on CS  and its protein sequence showed 100% identity with putative LR34 we annotated in this study (Fig. 2; Additional file 2).
Since numerous Arabidopsis genes have been characterized, especially for the ABC family, of which the major functions were largely known [21, 38, 53,54,55] (summarized in Additional file 3: Table S1), we then annotated these genes based on their best hit in the Arabidopsis genome (Additional file 2). For example, the putative LR34 in Ensmebl was annotated as ATPDR5 (Pleiotropic Drug Resistance 5, AT2G37280, also known as ABCG33 or ABC transporter G family member 33). Similarly, LR34-B (Traes_4AL_603B6DC64) was annotated as ATPDR9 (Pleiotropic Drug Resistance 9, AT3G53480, also known as ABCG37 or ABC transporter G family member 37). Thus, this annotation can be used to help select candidate genes for molecular characterization in wheat by leveraging the knowledge in the model plant.
Distribution of ABC, NLR and START genes in the three genomes of wheat and their physical proximity to SNPs of QTLs for leaf rust resistance
Of the ABC genes we predicted in this study, 165, 189 and 172 are from the A, B, and D genomes, respectively (Table 1). For NLR genes, 370 (CNL: 320, TNL: 3, N/A: 47), 395 (CNL: 346, TNL: 3, N/A: 46) and 352 (CNL: 313, TNL: 3, N/A: 36) are from A, B and D genomes respectively. For the START family: 45 (START: 15, HD-START: 3, HD-START-MEKHLA: 2, MINIMAL-START: 16, OTHER: 9), 51 (START: 20, HD-START: 4, HD-START-MEKHLA: 2, MINIMAL-START: 16, OTHER: 9), and 48 (START: 19, HD-START: 4, HD-START-MEKHLA: 3, MINIMAL-START: 15, OTHER: 7) are from A, B and D genomes respectively. The ABC, NLR and START genes were evenly distributed over the three wheat genomes (A, B and D), judging from insignificant chi-squared (χ2) tests for individual subfamily classes.
From the two data sets each with 2500 leaf rust resistance QTLs being downloaded from the T3 database , we retained 1090 and 1542 significant (P ≤ 0.01) QTLs for seedling and adult resistance, respectively. In these two data sets, a total of 1809 unique SNP markers were used (Additional file 4), and 451 of them are present in the QTLs of both seedlings and adult plants (Additional file 3: Fig. S1A). Because a total of 570 SNPs for seedling and adult resistance (representing 391 unique markers) have unknown (UNK) chromosomes (Additional file 3: Fig. S1B and C), we mapped their flanking sequences to the wheat genome to determine if these SNPs are located within the ABC, NLR and START genes analyzed in this study or their neighboring regions (5 Kb upstream of 5′ transcription start site [TSS] or downstream of 3′ transcription termination site [TTS] to the predicted genes).
The predicted ABC, NLR and START genes and the SNPs for seedling and adult resistance that reside within coding or neighboring (5 Kb upstream or downstream) regions of the predicted genes were mapped onto individual chromosomes in each of the three wheat genomes (A, B and D) (Fig. 3). The designated Lr genes previously mapped on each chromosome are also shown in the outer layer in Fig. 3 to indicate their physical affinity with the candidate genes and SNPs for leaf rust resistance. Unfortunately such affinity is very coarse due to the lack of mapping positions of the Lr genes on individual chromosomes. Some of the candidate genes with leaf rust SNPs only have positions on a scaffold (a set of ordered sequence contigs with gaps between them), and thus could not be depicted in Fig. 3 (Additional file 5). For this reason, we divided these SNPs into two categories: the genes with chromosome positions (WCP) and the genes with scaffold positions (WSP). The numbers of SNPs for seedling and adult resistance residing in coding regions of ABC, NLR and START genes or their neighborhood (within or 5 Kb upstream of 5′ end or downstream of 3′ end) showed different patterns (Table 2). For example, we found 18 SNPs in genic or neighboring regions of the ABC genes in seedlings and 39 in adult plants. Conversely, 29 and 14 SNPs for seedling and adult resistance were found in genic or neighboring regions of the NLR genes, respectively. In contrast, the START genes or their neighborhood contain roughly identical numbers of QTL SNPs for both seedlings and adult resistance.
Excluding the SNPs in non-coding and neighboring regions of ABC and NLR genes as shown in Table 2, we obtained a subset of SNPs that reside within coding regions and cause amino acid changes in these gene families (Table 3). These coding SNPs are the markers of QTLs identified for leaf rust resistance in wheat seedlings and adult plants as described above. The numbers of nonsynonymous SNPs in the coding regions of ABC and NLR genes exhibited different patterns in seedlings and adult plants. In the ABC genes, we found 17 missense SNPs for adult resistance and only five SNPs for seedling resistance. In contrast, in the NLR genes, we only predicted five nonsynonymous SNPs for adult resistance and six SNPs for seedling resistance (Table 3; Additional file 6). These candidate genes with nonsynonymous SNPs from the QTL data can be used in further studies to investigate their functional roles in Lr genes in wheat.
ABC, NLR and START genes linked to non-SNP molecular markers of rust resistance
The primer sequences of 99 non-SNP molecular markers linked to designated rust genes were used to identify corresponding candidate ABC, NLR and START genes in wheat. In total, we found 75 combinations of rust and Ensembl genes located near these markers, with the median physical distance of about 1.7 Mb (Additional file 7). Of these, eight genes are located less than 100 Kb away from the rust-associated markers, including two, five and one gene(s) in the ABC, NLR and START family, respectively (Table 4). For example, we found the gwm350 marker is only 293 bp to Traes_4AL_7AFBA6334. This gene might therefore be SrND643, a temporarily designated stem rust gene conferring resistance to several Ug99 races of Pgt . In addition, Lr34 (Yr18/Bdv1/Pm38/Ltn1) on 7DS was associated with Traes_7DS_25B02BA46, with a distance of 1212 bp to cssfr1 and 2189 bp to cssfr2, two known markers for Lr34 [13, 57]. Along with Fig. 2 (above-mentioned MSA), this result further supports the annotation of Traes_7DS_25B02BA46 as Lr34, demonstrating the usefulness of bioinformatics analyses in the discovery of rust resistance genes for functional characterization.
The expression differences of the putative ABC, NLR and START genes at different time points of infection (days post inoculation or dpi) in resistant and susceptible wheat plants are presented in Additional file 8. Our analysis revealed that the expression levels of the NLR genes for both resistant and susceptible plants were close to zero across all time points with the resistant plants being expressed only slightly more that the susceptible plants at comparable dpi (Fig. 4). In contrast, we observed higher levels of gene expression in the resistant wheat plants than in susceptible for ABC and START genes at early stages of infections (≤ 5 dpi) but susceptible plants had the similar amount of expression at the later stage (11 dpi). This is consistent with the finding in Fig. 3 of Dobon et al.  in which seven clusters of host genes were identified and one cluster (Cluster III) including the genes encoding ABC transporters had a peak expression at 11 dpi.
However, this result needs to be interpreted with care. Resistant and susceptible plants may have very different genetic backgrounds (i.e. with other rust resistance genes than Yr5 across the wheat genome) because they are different cultivars but not isogenic lines. Similar gene expression analyses were performed in the wheat cultivars Thatcher (susceptible) and its near-isogenic line (NIL) carrying a leaf rust resistance gene Lr9 (ThatcherLr9) or Lr10 (ThatcherLr10) (resistant) [58, 59], but both using an EST (expressed sequence tag) based approach. These analyses showed some genes were expressed at higher levels in the resistant cultivar, including an ABC transporter gene, while several NLR genes were expressed in both the susceptible and resistant samples infected with Ptr. Additionally, gene expression changes induced by the rust resistance gene Lr34/Yr18 were reported in the wheat NILs using Affymetrix wheat GeneChip microarray , which identified both upregulated and downregulated genes associated with resistance.
The present study employed a domain analysis of wheat proteome to predict a total of 1787 genes, with 526 coding for ABC proteins, 1117 for NLR proteins and 144 for START proteins over the three wheat genomes (A, B and D) (Table 1, Fig. 3). We were able to identify a number of SNPs that reside within protein-coding regions and cause amino acid changes of the predicted ABC, NLR and START genes or their neighborhood (5 Kb upstream from 5′ end or downstream from 3′ end). A total of 59 such SNPs were identified as the markers of QTLs for leaf rust resistance at the seedling stage and another 67 SNPs as the markers of QTLs for leaf rust resistance at the adult stage (Table 2; Fig. 3). The number of adult-resistance SNPs residing in the ABC genes or their neighborhood at the adult stage was more than doubled the number of seedling-resistance SNPs whereas the reverse pattern was observed for the SNP variants associated with the NLR genes (Table 2). This seems consistent with the expectation  that the QTLs for race-nonspecific, adult rust resistance are more likely to share genomic regions with the candidate genes in the ABC (and START) family whereas those for race-specific rust resistance in the seedlings are more likely to share genomic regions with the candidate genes in the NLR family.
This study represents a major step towards classification of the candidate genes for race-specific and race-nonspecific resistance in the three gene families and their chromosome-wise physical affinity with SNPs (and thus QTLs) for leaf rust resistance in wheat. Such information will be valuable for further characterizing QTLs at molecular level and their functional relationships with the ABC, NLR and START genes. We were already able to document our predicted amino acid changes due to alternation of single SNPs (point mutation) in the coding regions of the ABC, NLR and START genes (Fig. 2; Table 3). Our analysis was motivated by recent reports that changes in two amino acids caused the difference in proteins encoded by the resistance and susceptibility alleles of Lr34 [6, 15] and Lr67 . Nevertheless, our prediction based on point mutation must be treated as a preliminary result as structural variations such as copy number variation (CNV) of resistance genes may possess a more dramatic impact on disease and other biotic stress . Further complication arises from temporal variation among expressions of the putative ABC, NLR and START genes in compatible (susceptible) and incompatible (resistant) wheat-rust interactions as evident in our RNA-Seq analysis (Fig. 4). Thus, the predictions based on point mutation such as ours can only serve as a starting point towards more thorough molecular and functional characterization of resistance genes for their eventual cloning.
Our prediction indicates that the number of race-specific NLR genes (R genes) is almost doubled the number of race-nonspecific genes (ABC and START genes). This seems consistent with the phenomenon that the majority of Lr genes identified so far in wheat are effective against single races of lest rust only. As Krattinger et al.  pointed out, this phenomenon is also true for resistances to other diseases in wheat and other cereals. The problem with the use of such race-specific Lr genes deployed in the wheat cultivars is that they have quickly become ineffective when new, more virulent races appear in leaf rust pathogen . For this reason, wheat breeders and pathologists have focused on discovery, characterization and use of race-nonspecific genes for durable resistance. However, decades of genetic and breeding research have only been able to identify a limited number of genes in wheat with durable and broad-spectrum resistance to rusts including Lr34/Yr18/Sr57/Pm38, Lr46/Yr29/Pm39, Lr67/Yr46/Sr55/Pm46, Lr68, Lr75, and Yr36 [5, 6, 15, 16, 61, 64, 65]. Thus far, Lr34/Yr18/Sr57/Pm38 (or simply Lr34 hereafter) is the most well-characterized multi-pathogen resistance gene located on wheat chromosome 7D . Unfortunately we did not find any known SNPs for the leaf rust resistance in the T3 data sets  that would reside within coding regions of the Lr34 gene or its neighborhood within 5 Kb upstream and downstream. Certainly, the wheat 9 K and 90 K SNP chips are hardly sufficient to cover large, complex wheat genomes, particularly the D genome (cf. Fig. 3). With ongoing international efforts on manifold sequencing of wheat genomes, exact physical positions and functions of those SNP variants within the ABC genes (Table 2) will be further clarified and characterized.
It may be questioned why Lr67/Yr46/Sr55/Pm46 (or simply Lr67) was not included in this study as it is another well-characterized race-nonspecific gene at the molecular level just like Lr34. The reason is that it does not encode an ABC transporter, but rather it encodes a hexose transporter and confers a similar, but somewhat reduced partial resistance to all three wheat rust pathogens and powdery mildew . Like in Lr34, only two amino acid substitutions (Arg144Gly and Leu387Val) distinguish the resistance (LR67res) from susceptibility (LR67sus) protein . The hexose transport activity of LR67 has been demonstrated in a yeast (Saccharomyces cerevisiae) mutant lacking the glucose uptake ability [62, 66]. A preliminary analysis of five protein sequences encoded by Lr67  including three homoeologs (in A, B, D chromosomes) and the resistance/susceptibility versions from GenBank (accessions: ALL26327, ALL26328, ALL26329, ALL26330 and ALL26331) showed that none of them exhibited a high level of homology to the three family proteins (ABC, NLR and START) analyzed in this study (E-value >1 × 10−5 and sequence identity <36%). Since LR67 belongs to the sugar transporter protein (STP) family, a genome-wide search of the STP proteins using the major facilitator, sugar transport domain (InterPro accession IPR005828, a domain present in all the above five LR67 proteins), allowed for discovering 261 STP proteins (E-value <1 × 10−5) in wheat. This STP data set along with the ABC and other possible transporter families can be further examined in future studies for their physical relationships with QTLs for partial, adult resistance to multiple pathogens in wheat and other cereals.
This study has focused on annotating and characterizing the physical relationships between ABC, NLR and START genes and QTLs for resistance to leaf rust. Similar efforts can be made for stem and stripe (yellow) rusts. At the time of completion of this work as of February 2017, there is only a limited amount of QTL data available for stem and stripe rust resistance from the T3 database  that can be used for such annotation and characterization; in particular, imbalanced availability of QTLs for seedling and adult resistance makes a valid comparative assessment more difficult. As more QTL data become available for stem and stripe rusts, it is conceivable that a similar pattern of the physical relationships between the gene families and QTLs will be identified, just like what we observed in leaf rust.
Our sequence analyses were greatly affected by the fragmentary status of the draft wheat genome assembly . In the Ensembl release 31 of the wheat genome used in this study, the 21 wheat chromosomes were represented by a total of 317,977 assembled scaffold sequences. The average scaffold length was about 20,389 bp (S.D. ± 222 7384 bp), with the median of 3800 bp and maximum of 774,434,471 bp. Because of similar concerns about assembly quality, we chose not to use Ensembl release 33 (the latest release in October 2016), which incorporated short RNA-seq reads and full-length cDNA sequences  but seems more fragmented than release 31 according to our assessment. For example, the wheat genome assembly in release 33 consists of 735,943 supercontig sequences (compared with 317,977 in release 31), with an average supercontig length of 18,245 bp (S.D. ± 41,822 bp), median of 2431 bp and maximum of 823,974 bp. The fragmentary assembly of the wheat genome might also have affected our BLAST searches using the primer sequences against the wheat genomic sequences, and this may be the primary reason of why only seven ABC, NLR and START genes were identified within 100Kb to the markers of QTLs for rust resistance (Table 4, Additional File 7). Other studies based on similar fragmentary assemblies were not even able to identify candidate genes but rather some genomic regions for disease resistance and other agronomic traits. Two such studies are: (i) an in silico mapping of DArT marker sequences for identification of genomic regions harboring resistance to the three rusts  and (ii) flanking sequences of the SNPs of the wheat 90 K chip for identification of candidate genes and regions for pre-harvest sprouting (PHS) resistance in wheat , but with the candidate genes being inferred from Brachypodium (Brachypodium distachyon) and rice genomes via comparative mapping.
In addition to the ABC, NLR and START genes analyzed in this study, genes in other families may also be involved in rust resistance. We have already mentioned genes within the STP transporter family like Lr67. Additional genes include those encoding unusual kinases, receptor-like kinases (RLKs) and receptor-like proteins (RLPs) , and genes involving cell wall biosynthesis and metabolism, such as cysteine proteinase, phenylalanine ammonia-lyase, plasma membrane ATPase and chalcone synthase . When a pathogen attacks its host, the first line of host defense is through secretion of cell surface pattern-recognition receptors (PRRs) and such defense-induced proteins may be recognized and overcome by specific pathogen effectors. The majority of characterized PRRs are either RLKs or RLPs [70,71,72]. A recently developed tool called RGAugury  focused on classification of these proteins and NLR protein family. However, our study had a different focus on the three major protein families related to leaf rust resistance in wheat and these families contain different domains suitable for large-scale analyses.
Certainly with ongoing international efforts for more in-depth wheat genome sequencing, the candidate genes we identified for each of the three gene families eventually will be physically mapped at accurate locations on individual chromosomes. In other words, Fig. 3 will be significantly clarified and updated. Such updating will enable wheat breeders and pathologists to use the well-characterized ABC, NLR and START genes for molecular characterization of the designated Lr genes in breeding for rust resistances. While ABC and START genes may be preferably used as reasoned above, their resistance is only partial and insufficient under high disease pressure when used alone. Strong, durable resistance has been achieved by combining these race-nonspecific genes with race-specific NLR genes such as ‘Sr2-complex’ . However, little is known about the other combining genes and their interactions in the complex. Thus, it would be desirable that future studies will investigate how race-nonspecific genes in the ABC or START gene family interact with weak and strong R genes in the NLR gene family at the molecular level using the candidate genes we identified and characterized for the three gene families.
In this study we predicted the putative ABC, NLR and START genes in the hexaploid wheat genome, and performed an integrated analysis with the available genetic resources related to rust resistance, including mapped Lr genes, SNPs and other molecular markers. Our analysis suggests that the ABC (and START) genes are more likely to share genomic regions with QTLs for race-nonspecific, adult resistance whereas the NLR genes are more likely to share genomic regions with QTLs for race-specific resistance that would be often expressed at the seedling stage. Candidate genes such as those containing one or multiple missense coding SNPs can be tested in future studies. With continuing efforts for producing an improved assembly and annotation of the wheat genome, bioinformatics analyses such as ours can help identify novel genes for rust resistance breeding in wheat.
Prediction of ABC, NLR and START genes in wheat genome
The wheat proteome (release 31) was downloaded from the FTP archive site (ftp://ftp.ensemblgenomes.org/pub/release-31/plants/fasta/triticum_aestivum/pep/Triticum_aestivum.IWGSC1+popseq.31.pep.all.fa) of Ensembl Plants . This database contains a total of 100,344 predicted protein sequences. Using these protein sequences, we predicted the domains within each protein using the standalone software InterProScan (version 5.21–60.0, 64-bit) , installed on a CentOS (release 7.2) Linux system with 8-core CPU (1600 MHz) and 32 Gb RAM. The InterProscan E-value cutoff was set to 1 × 10−5 . From the InterProscan output file, the presence of PF00005 (ATP-binding domain of ABC transporters) from the protein family database Pfam  within a protein sequence was used to select candidate ABC proteins. These candidates were then classified into eight families (A, B, C, D, E, F, G and I) based upon their best BLASTP hit against the known ABC proteins in Arabidopsis and Oryza sativa (rice), which were collected from the literature [54, 75]. The G family was further divided into Gwbc (White Brown Box) and Gpdr (Pleiotropic Drug Resistance). For the START family, we used the presence of the START domain (InterPro: IPR002913 and IPR005031) or START-like domain (IPR023393) as the signature, and grouped the putative START proteins into five categories: MINIMAL-START, START, HD-START, HD-START-MEKHLA and OTHER. This classification scheme is similar to those reported in previous studies [22, 48]. A MINIMAL-START protein only contains a single START or START-like domain, whereas a START protein contains multiple domains with at least one START or START-like domain. Moreover, a multidomain START protein was designated as HD-START if it contains a homeodomain-like domain (IPR009057) and as HD-START-MEKHLA if it also contains a MEKHLA domain (IPR013978). The NBS-LRR resistance proteins were predicted using the NLR-Parser tool  in a two-step process. First, the entire wheat proteome was scanned with the MEME-formatted motifs (meme.xml) using MAST (Motif Alignment and Scanning Tool) in the MEME (version 4.9.1) suite . Second, the MAST output (mast.xml) was parsed and the NBSLRR genes were classified with NLR-Parser into three classes: CNL (COIL-NBS-LRR), TNL (TIR-NBS-LRR) and N/A.
In silico mapping of the QTL SNP markers to ABC, NLR and START genes on the wheat chromosomes
Two data sets, each with 2500 quantitative trait loci (QTLs) for leaf rust resistance at seedling or adult stage, were extracted from the “GWAS Results” in the Triticeae Toolbox (T3) database (https://triticeaetoolbox.org/wheat/qtl/qtl_report.php) . By selecting ‘Biotic stress’ in the “Category” column and by clicking appropriate leaf rust traits at adult or seedling stage in the “Traits” column, the T3 would display the results from the GWAS analysis for associations between markers (including Infinium 9 K, Infinium 90 K, and GBS restriction sites) and traits for individual trials (individual locations or inoculum types) within the T3 database. The GWAS analysis was carried out using rrBLUP GWAS package  for individual trials or the combined analysis across all trials with the genotype-by-environment interaction effect being adjusted by including those principle components that accounted for more than 5% of the environment-relationship matrix variance as fixed effects in the mixed-model analysis. For a better control of false positive rates in these preliminary QTL data sets, we applied a significance level of P ≤ 0.01 which allowed for retaining 1090 QTLs in seedlings and 1542 QTLs in adult plants for subsequent analysis (Additional file 4).
The sequences of the SNP markers from the GWAS results described above were obtained in T3 and CerealsDB . To predict their functional consequences, these SNPs were first converted into a VCF (Variant Call Format) and annotated with VEP . Then, to better estimate the genomic position of each SNP and its proximity to the predicted genes in the ABC, NLR and START gene families, we mapped the sequence (removing the ‘/’, B allele and the surrounding square brackets) flanking each SNP by alignment to the wheat genome assembly (Triticum_aestivum.IWGSC1 + popseq.31.dna_sm.toplevel.fa) using BLASTN (version 2.5.0+) in the BLAST+ package . This assembly was generated by Ensembl Plants from the whole genome shotgun sequencing data , a chromosome-based draft sequence  and the POPSEQ (population sequencing) data . The chromosomal positions of these genes were based on the Ensembl annotation file (Triticum_aestivum.IWGSC1 + popseq.31.gff3) in GFF3 (General Feature Format) format. Both files were downloaded from the Ensembl FTP archive site (ftp://ftp.ensemblgenomes.org/pub/release-31/plants/). As each flanking sequence often matches several genomic regions, we used E-value, alignment identity and query coverage as criteria to retain the top three candidates that usually represent one hit in each of the three wheat genomes.
For the SNPs located in the genomic regions covering the ABC, NLR and START genes, those in the coding regions were recognized through aligning their flanking sequences with their coding sequence (CDS) downloaded from Ensembl. We then compared the two alleles of each SNP marker with the allele at the same position of the Ensembl CDS and determined if the Ensembl allele (as reference) was the same as the A or B allele at the SNP site, or in some cases differed from both alleles. To predict the amino acid change, we replaced the Ensembl CDS with the A or B allele, translated the newly generated CDS into protein sequence using the standard codon table. Therefore, if the Ensembl reference allele matches the A or B allele at the SNP site, two protein sequences were generated and otherwise three protein sequences were translated for each SNP marker. To verify this process, we utilized the known SNP (and deletion) in the Lr34 CDS as well as the resistant and susceptible LR34 proteins [6, 15]. We then found the amino acid difference at the corresponding position of each protein translated from each CDS containing different SNP alleles.
Prediction of candidate genes based on sequences of non-SNP markers in wheat
We collected a set of 99 non-SNP markers that showed significant (a LOD score of ≥3.0) associations with wheat rust resistance from Liu et al.  and other sources. The primer sequences of these markers were found in GrainGenes , MASWheat (http://maswheat.ucdavis.edu/protocols/stemrust/) and the literature (Additional file 7). Similar to the above analysis of SNP flanking sequences, we searched the primer sequences against the wheat genome sequence assembly (Triticum_aestivum.IWGSC1 + popseq.31.dna_sm.toplevel.fa) using BLASTN  to identify genomic regions covered by these markers. Each primer query was generated by concatenating the forward primer and reverse primer with five ‘N’ letters inserted between them as gaps. Because of the short length of each primer pair (usually less than 40 bp), blastn-short, a BLASTN program optimized for sequences shorter than 50 bases , was used with settings of a word size of seven and a relaxed E-value of 100. To compare chromosomal positions of markers and candidate genes, we also used the Ensembl annotation file (Triticum_aestivum.IWGSC1 + popseq.31.gff3).
Dobon et al.  recently published a RNA-seq time-course data set for a resistant line (Avocet-Yr5) and a susceptible wheat cultivar (Vuka) inoculated with yellow rust pathogen Puccinia striiformis f. sp. tritici (Pst). The rust inoculum (isolate 87/66 of Pst) is avirulent to the resistant line (Avocet-Yr5), but virulent to the susceptible cultivar (Vuka). We analyzed the expression values (Transcripts Per Million reads or TPM) of 123,532 wheat transcripts (based on the annotation of Ensembl Plants ) taken from Supplemental Tables S20 and S21 of Dobon et al. . We identified putative ABC, NLR and START genes from those host transcripts and analyzed their expression profiles at different days post inoculation (dpi) in the resistant and susceptible wheat plants. The original data on the expression profiles contain five time points (0, 1, 2, 3, and 5 dpi) for Avocet-Yr5, and eight (0, 1, 2, 3, 5, 7, 9, and 11 dpi) for Vuka. We then extracted the TPM values for the putative ABC, NLR and START genes, eliminating those with zero values on all dpi. For each of the putative genes in the three gene families, we calculated a fold change as the sum of the five expression values in the resistant host divided by the sum in the susceptible host. In addition, we identified ABC, NLR and START genes expressed exclusively in the resistant host or the susceptible host.
days post inoculation
nucleotide-binding site /leucine-rich repeat (NBS-LRR)
pleiotropic drug resistance
quantitative trait locus
single nucleotide polymorphism
steroidogenic acute regulatory (StAR) protein-related lipid transfer domain
white brown complex
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We wish to thank three reviewers for critical and constructive comments on an earlier draft, and Dr. Zhiqiu Hu for helpful discussion and for his help with the preparation of Fig. 3.
This research is funded by the Growing Forward 2 Research Opportunities and Innovation Internal Initiatives of Alberta Agriculture and Forestry to R-C Yang.
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All supporting data can be found within the manuscript and its additional files.
Conceived and designed the analyses: RCY and YFP; Performed the analyses: FYP and RCY; Writing of the manuscript: YFP and RCY; Both authors read and approved the final version of the manuscript.
The authors declare that they have no competing interests.
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Summary of the family classification of the ABC, NLR and START proteins in wheat and their predicted InterPro domains as well as the description for each InterPro identifier. (XLSX 141 kb)
Annotation of the putative ABC, NLR and START genes in wheat using the sequences of plant resistance genes in GenBank and PRDdb. (XLSX 228 kb)
Summary of the ABC transporter family, domain structures and major functions in plants. Table S2. The VEP annotation of the 1809 unique SNPs associated with leaf rust resistance in seedling and adult wheat plants from the T3 database. Figure S1. The Venn diagram (A) of the SNP markers of leaf rust resistance QTLs in seedlings and adult plants in the T3 database and their distribution on different wheat chromosomes (B: seedlings; C: adult plants). (PDF 574 kb)
The leaf rust resistance QTLs (P ≤ 0.01) from the T3 database of the wheat seedlings and adult plants and the flanking sequences of their SNP markers from CerealsDB and T3 database. (XLSX 335 kb)
The SNP markers located within (marker located between the gene start and the gene end on the same chromosome; highlighted) ABC, NLR and START genes and 5 Kb upstream (from transcription start site or TSS) or downstream (from transcription termination site or TTS) of them. (XLSX 78 kb)
Predicted effects of rust SNPs on the amino acid changes in the proteins encoded by the putative ABC, NLR and START genes. Genes with multiple SNPs were highlighted. (XLSX 50 kb)
The molecular markers and their primer sequences found in GrainGenes and candidate genes near these makers. (XLSX 22 kb)
RNA-seq expression values (in unit of TPM or transcripts per million) of ABC, NLR and START genes in resistant and susceptible wheat hosts at five time points (0 dpi, 1 dpi, 2 dpi, 3 dpi and 5 dpi or days post inoculation), fold changes of genes expressed in both resistant and susceptible wheat, and genes expressed only in the resistant or susceptible wheat. (XLSX 211 kb)
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Peng, F.Y., Yang, R. Prediction and analysis of three gene families related to leaf rust (Puccinia triticina) resistance in wheat (Triticum aestivum L.). BMC Plant Biol 17, 108 (2017) doi:10.1186/s12870-017-1056-9
- ABC transporter
- NLR (NBS-LRR)
- Rust resistance genes
- Molecular markers
- Single nucleotide polymorphism (SNP)
- Bread wheat
- Triticum aestivum
- Genome analysis