Fig. 6

sqRT-PCR of ST1, ST2 and ST3 transcripts in Medicago truncatula seedlings under different treatments. a Transcript accumulation in untreated 7-d-old seedlings (control) and 7-d-old seedlings exposed during the last 24 h to indolacetic acid (IAA, 10 μM); benzylaminopurine (CK, 10 μM); giberellic acid (GA, 100 μM); epibrassinolide (BL, 10 μM) and strigolactone GR24 (SL, 10 μM). b Transcript accumulation in untreated 7-d-old seedlings (control) and 7-d-old seedlings exposed during the last 24 h to abscisic acid (ABA, 100 μM); sodium chloride (NaCl, 150 mM); mannitol (250 mM); N starvation (−N) plants where kept for 7 days in Fahräeus without N supplement and Pi starvation (−Pi) plants where kept for 7 days in Fahräeus-N without phosphate. c Transcript accumulation in plants exposed at different temperatures: 25 °C (control), −4 °C and 37 °C in darkness for the last 12 h in 7-d-old seedlings. d: Transcript accumulation in untreated 7-d-old seedlings (control) and 7-d-old seedlings exposed during the last 24 h to ethephon (ET, 1 mM); methyl jasmonate (MeJA, 100 μM); salicylic acid (SA, 1 mM); as well as a mixture of MeJA with one of the following: ET (MeJA + ET); SA (MeJA + SA) and GA (MeJA + GA) at the same concentrations as when individually applied. Wounding was performed in one foliole by transversally cutting the middle vein. Transcript accumulation was measured relative to ubiquitin as indicated in the Methods section. Scale units express normalized and integrated absorbance (nIA). Statistical analyses were conducted as stated in the Methods section considering 3 levels of significance: *p < 0.05; **p < 0.01; ***p < 0.001