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Fig. 4 | BMC Plant Biology

Fig. 4

From: Suppression of the phytoene synthase gene (EgcrtB) alters carotenoid content and intracellular structure of Euglena gracilis

Fig. 4

Effects of suppressing EgcrtB on E. gracilis cells. Cells treated with or without EgcrtB-dsRNA were cultured for 7 days at 25 °C under continuous illumination at 55 μmol m−2 s−1. a Semi-quantitative reverse transcription-PCR analysis of EgcrtB expression in cells with (+) or without (−) EgcrtB-dsRNA. Non-electroporated cells (n) were used as the control. GAPDH expression was used as an internal control for the semi-quantitative RT-PCR analysis. b Cells treated with or without EgcrtB-dsRNA and visual appearance of culture medium (insets) after cultivation for 3 and 7 days. Scale bar, 20 μm. c TEM images of intracellular structures (left column: scale bar, 5 μm) and sections of chloroplasts (right column: scale bar, 500 nm) of cells treated with or without EgcrtB-dsRNA. C, chloroplast; CV, contractile vacuole; LG, lipid globule; N, nucleus; P, paramylon; PG, plastoglobule

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