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Table 2 Effects of suppressing EgcrtB on cell concentration and chlorophyll content of E. gracilis

From: Suppression of the phytoene synthase gene (EgcrtB) alters carotenoid content and intracellular structure of Euglena gracilis

Treatment

Cell concentration

Chlorophyll content (nmol 106 cells−1)

Chlorophyll a/b

Cultured for 3 days (×104 cells ml−1)

Cultured for 7 days (×106 cells ml−1)

a

b

non-electroporated

7.7 ± 0.7a

1.9 ± 0.0a

7.7 ± 0.9a

1.1 ± 0.1a

6.9 ± 0.3a

EgcrtB-dsRNA(−)

5.5 ± 0.7b

1.8 ± 0.1a

8.5 ± 1.6a

1.2 ± 0.2a

7.3 ± 0.3a

EgcrtB-dsRNA(+)

3.3 ± 0.2c

0.3 ± 0.0b

1.3 ± 0.1b

0.2 ± 0.0b

6.1 ± 0.5b

  1. Data represent mean ± SD of biological replicates. The number of biological replicates was as follows: cell concentration, n = 3; chlorophyll content and chlorophyll a/b ratio, n = 4. Different letters in each column indicate a significant differences (P < 0.05, Tukey’s test)