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Fig. 6 | BMC Plant Biology

Fig. 6

From: Characterization of four rice UEV1 genes required for Lys63-linked polyubiquitination and distinct functions

Fig. 6

Subcellular distribution of selected OsUev1s. a Subcellular localization of GFP-OsUev1s and their derivatives as illustrated in the right panel. The GFP-tagged OsUev1s were expressed in N. benthamiana leaves by an Agrobacterium-mediated infiltration method. Photos were taken after 2–3 days of infiltration. A representative image is shown for each transformant. b The above transformed N. benthamiana leaves were analyzed by a protein-partitioning assay as described in Methods. GFP-OsUev1A and GFP-OsUev1A-∆CT were detected by an anti-GFP antibody (B-2, sc-9996, Santa Cruz). A: aqueous phase; D: detergent phase

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BMC Plant Biology

ISSN: 1471-2229

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