Fig. 3

Quantitative RT-PCR analysis of CsTIR1 and CsAFB2 expression in cucumber. a, b CsTIR1 and CsAFB2 expression in different organs of cucumber (roots, stems, leaves, female flowers, male flowers, and young fruit). c, d CsTIR1 and CsAFB2 expression in cucumber leaves treated with GA3 (10 μM), 6BA (10 μM), and different concentrations of NAA (5, 10, and 50 μM). e, f CsTIR1 and CsAFB2 expression in cucumber ovaries under pollination and treatment with CPPU (400 μM), NAA (500 μM), GA3 (3000 μM), and BRs (0.2 μM). The “young fruit” represent fruit at 0 days post-anthesis. CK, “Control”. Data represent mean ± SD normalized relative to CsActin gene transcript levels. Root and CK expression data normalized to 1. All samples were analyzed in triplicate. Expression differences are calculated relative to expression in the root (a, b) and CK (c, d, e, f). (Student’s t test; * P < 0.05; ** P < 0.01)