Fig. 8

Expression analysis of full-length transcripts and total transcripts of CsTIR1 and CsAFB2 during the cucumber parthenocarpic fruit set, pollination fruit set and fruit abortion processes. a Schematic diagram of specific primer location. CsTIR1 has three nucleotide mismatches in the miR393 recognition site, while CsAFB2 contains two mismatches in the miR393 recognition site. The orange pair of primers separated on both sides of the miR393-cleavage site were used to detect the full-length transcripts of CsTIR1 and CsAFB2. The green pair of primers distributed on one side of the miR393-cleavage site were used for detection of total transcripts of CsTIR1 and CsAFB2. b Semi-quantitative RT-PCR analysis of full-length transcript and total transcript accumulation of CsTIR1 and CsAFB2 during the early stages of the cucumber parthenocarpic fruit set, pollination fruit set and fruit abortion processes (−3 dpa to 3 dpa). dpa: days post-anthesis. fm: full-length mRNA (complete fragments); tm: total mRNA (complete fragments and alternative splicing fragments)