- Research article
- Open Access
Comparative studies on tolerance of rice genotypes differing in their tolerance to moderate salt stress
© The Author(s). 2017
Received: 17 January 2017
Accepted: 7 August 2017
Published: 17 August 2017
Moderate salt stress, which often occurs in most saline agriculture land, suppresses crop growth and reduces crop yield. Rice, as an important food crop, is sensitive to salt stress and rice genotypes differ in their tolerance to salt stress. Despite extensive studies on salt tolerance of rice, a few studies have specifically investigated the mechanism by which rice plants respond and tolerate to moderate salt stress. Two rice genotypes differing in their tolerance to saline-alkaline stress, Dongdao-4 and Jigeng-88, were used to explore physiological and molecular mechanisms underlying tolerance to moderate salt stress.
Dongdao-4 plants displayed higher biomass, chlorophyll contents, and photosynthetic rates than Jigeng-88 under conditions of salt stress. No differences in K+ concentrations, Na+ concentrations and Na+/K+ ratio in shoots between Dongdao-4 and Jigeng-88 plants were detected when challenged by salt stress, suggesting that Na+ toxicity may not underpin the greater tolerance of Dongdao-4 to salt stress than that of Jigeng-88. We further demonstrated that Dongdao-4 plants had greater capacity to accumulate soluble sugars and proline (Pro) than Jigeng-88, thus conferring greater tolerance of Dongdao-4 to osmotic stress than Jigeng-88. Moreover, Dongdao-4 suffered from less oxidative stress than Jigeng-88 under salt stress due to higher activities of catalase (CAT) in Dongdao-4 seedlings. Finally, RNA-seq revealed that Dongdao-4 and Jigeng-88 differed in their gene expression in response to salt stress, such that salt stress changed expression of 456 and 740 genes in Dongdao-4 and Jigeng-88, respectively.
Our results revealed that Dongdao-4 plants were capable of tolerating to salt stress by enhanced accumulation of Pro and soluble sugars to tolerate osmotic stress, increasing the activities of CAT to minimize oxidative stress, while Na+ toxicity is not involved in the greater tolerance of Dongdao-4 to moderate salt stress.
Soil salinity is a global environmental challenge, limiting crop production over 800 million hectares worldwide . The majority of the saline land has arisen from natural events and human intervention, such as release of soluble salts of various types during weathering of parental rocks, the deposition of oceanic salts by wind and rain as well as irrigation containing trace amounts of sodium chloride . Rice is an important cereal that provides 50–80% of daily calorie intake for more than 3 billion people. Rice plants are sensitive to salt stress, particularly at the seedling and reproductive stages. However, rice genotypes differ in their sensitivity to salt stress, and some rice genotypes tolerant to salt stress have been reported, including those of genotypes Pokkali  and IR63731–1–1-4-3-2 . Elucidating of the molecular and physiological mechanisms by which rice genotypes respond and adapt to salt stress are pivotal for selecting and breeding rice genotypes capable of growth in the saline soils.
Plants suffering from high salt stress often display symptoms of Na+ toxicity due to accumulation of Na+, which in turn reduces nutrient acquisition, leading to nutritional imbalances, and oxidative damage . Plants have evolved several mechanisms to cope with these problems. Minimizing Na+ toxicity by compartmenting toxic Na+ into vacuoles and/or restricting Na+ uptake by plants are the most common strategy for plants to tolerate salt stress . For example, greater tolerance to Na+ confers barley greater tolerance to salt stress than wheat despite similar foliar Na+ concentrations in barely to those of wheat .
In addition, plants exposed to salt stress can also suffer from osmotic stress. Therefore, plants have to equip with capacity to tolerate osmotic stress under saline conditions. Salt stress limits plant growth by increasing the osmotic potential of the soil and, thus, decreasing water uptake by the roots. Accumulation of compatible osmolytes in the cytosol, lowering osmotic potential to sustain water absorption from saline soil solutions, is an important salinity tolerance mechanism [7, 8]. Many attempts to molecular breeding plants tolerant to drought have been made by introducing genes that encode key enzymes for biosynthesis of compatible solutes .
Increases in activities of enzymes that detoxify reactive oxygen species also contribute to plant tolerance to salinity . For example, Mishra et al. (2013) reported that salt tolerant rice seedlings have a better protection against reactive oxygen species (ROS) by increasing the activities of antioxidant enzymes under salt stress . Transgenic plants overexpressing genes encoding antioxidant enzymes are more tolerant to salt stress than their wild-type counterparts [12, 13].
Several Na+ transporters responsible for Na+ uptake, translocation and compartmentation have been identified in rice plants, including those OsHKTs, OsHAKs, OsNHXs [14–16]. However, the molecular mechanisms underlying Na+ transport from soil solution and within plants remain largely elusive. A comprehensive genome-wide analysis of gene expression in response to salt stress may shed some light on molecular mechanisms responsible for tolerance of rice plants to salt stress. RNA-seq, a high-throughput sequencing technology, was widely used to dissect transcriptomic information. These transcriptome-wide studies have provided new insights on genes and regulatory mechanisms involved in abiotic stresses.
Soil is often referred to as saline one when the electrical conductivity (equivalent to the concentration of salts in saturated soil or in a hydroponic solution) is greater than 4 dS m−1, which is equivalent to approximately 40 mM NaCl and yields of most crops are suppressed when grown in such saline soils . However, higher levels of concentration of NaCl (150–200 mM) have been frequently used to study the physiological and molecular mechanisms to saline stress in the literature [3, 17, 18]. Therefore, elucidating the mechanisms underlying response and tolerance to moderate salt stress that is similar to natural saline soils will be of practical implications regarding for breeding crops capable of growing in saline soils. In our previous studies, we collected more than 100 rice genotypes and assessed their tolerance to saline-alkaline stress. Among these rice genotypes, Dongdao-4 is an elite rice genotype that is capable of growing in saline-alkaline soils in the northeast of China, while the genotype Jigeng-88 is a relatively saline-alkaline-sensitive genotype. In addition, we have indicated that Dongdao-4 rice genotype is more tolerant to saline-alkaline stress than Jigeng-88 by more efficient acquisition of iron under saline-alkaline conditions . However, whether the two rice genotypes differ in their tolerance to moderate, neutral salts stress is unclear. In the present study, we made a comparative study on the effects of moderate salt stress on the two genotypes differing in their tolerance to saline-alkaline stress. We further investigated the physiological and molecular mechanisms responsible for salt tolerance in rice plants.
Dongdao-4 seedlings are more tolerant to moderate salt stress than Jigeng-88 seedlings
To characterize the differences in salt tolerance between Dongdao-4 and Jigeng-88 plants, three-week-old rice seedlings of the two rice genotypes were exposed to solution supplemented with 20 mM NaCl for one day, and 40 mM NaCl for another day, and then exposed to 60 mM NaCl for one week. We monitored the effects of salt stress on plant growth (Additional file 1: Figure S1), photosynthetic rates (Additional file 1: Figure S2b), and Na+, K+ concentrations (Additional file 1: Figure S3). Our results showed that, similar to previous results of saline-alkaline stress , Dongdao-4 was more tolerant to moderate salt stress than Jigeng-88. Chlorophyll content in Dongdao-4 plants was little affected by saline stress, while a significant reduction in chlorophyll content in Jigeng-88 plants was observed by salt stress, leading to a significantly higher chlorophyll content in Dongdao-4 than in Jigeng-88 when exposed to salt stress (Additional file 1: Figure S2a).
Dongdao-4 plants accumulated more soluble sugars and Pro
To further elucidate the mechanisms by which Dongdao-4 accumulated greater amounts of Pro than Jigeng-88 under salt stress, the effects of salt stress on the expression of genes responsible for Pro biosynthesis (Δ1-pyrroline-5-carboxylate synthase genes, Os05g0455500 and Os01g0848200), Pro transport (Os03g0644400 and Os07g0100800) were investigated. As shown in Fig. 1c, treatment with salt stress led to a great increase in transcripts of Os05g0455500 in Dongdao-4 seedlings, whereas expression levels of Os05g0455500 in Jigeng-88 seedlings remained relatively unchanged, leading to a significantly higher expression level of Os05g0455500 in Dongdao-4 than in Jigeng-88 plants. Exposure to salt stress led to a similar increase in transcripts of Os01g0848200 in the two genotypes (Fig. 1d). Treatment with salt stress led to a significant increase in transcripts levels of Os03g0644400 in both genotypes, with the magnitude of increase in Dongdao-4 greater than in Jigeng-88 (Fig. 1e). Expression levels of Os07g0100800 in Dongdao-4 were significantly lower than those in Jigeng-88 under control conditions (Fig. 1f). Exposure to salt stress led to a significant increase in expression level of Os07g0100800 in Dongdao-4 plants (Fig. 1f), while expression level of Os07g0100800 in Jigeng-88 plants was constant upon exposure to salt stress, leading to no differences in expression levels of Os07g0100800 in the two genotypes under conditions of salt stress (Fig. 1f).
Effect of mannitol on Dongdao-4 and Jigeng-88
Survival rate, Dry shoot biomass, Dry root biomass, Shoot soluble sugar, Shoot Pro of Dongdao-4 and Jigeng-88 plants before, after exposure to medium supplemented with mannitol and recovery from the mannitol treatment. Three-week-old seedlings were transferred to solution containing 40 mM mannitol for 1 day, and 80 mM mannitol for 1 day, and then exposed to 120 mM mannitol for 5 days, finally recovered for 15 days. Data are means ±SE (n ≥ 4). Means with different letters are significantly different (P < 0.05) within the same treatments. Asterisks (*) indicate significant differences between control and treatments of the same genotype which were determined by Student’s t-test (*0.01 < P < 0.05, **0.001 < P < 0.01, ***P < 0.001)
120 mM Mannitol
Survival rate (%)
100 ± 0.00
100 ± 0.00
100 ± 0.00***a
72.92 ± 3.45b
Dry shoot biomass (mg plant−1)
113.12 ± 4.47
100.68 ± 3.49
132.18 ± 7.68 **a
83.53 ± 2.99 *b
526.08 ± 18.83 ***a
175.03 ± 13.82 *b
Dry root biomass (mg plant−1)
12.81 ± 0.12
12.5 ± 0.62
19.95 ± 0.88**a
12.73 ± 0.35b
66.23 ± 3.85***a
16.48 ± 0.66b
Shoot soluble sugar (mg g−1 DW)
1.79 ± 0.11
1.94 ± 0.43
16.61 ± 0.73***a
13.52 ± 0.41***b
Shoot proline (mg g−1 DW)
0.67 ± 0.06
0.75 ± 0.06
1.32 ± 0.14**a
0.64 ± 0.02b
The two genotypes exhibited a comparable shoot soluble sugars and Pro contents in the absence of mannitol (Table 1). There was a significant increases in shoot soluble sugars and Pro contents in both genotypes upon exposure to the medium supplemented with 120 mM mannitol, whereas the magnitude of treatment-induced increases in shoot soluble sugars and Pro contents in Dongdao-4 plants was greater than that in Jigeng-88 plants, leading to a significantly higher shoot soluble sugars and proline contents in Dongdao-4 than that in Jigeng-88 seedlings under osmotic stress conditions (Table 1).
Dongdao-4 plants exhibited greater tolerance to oxidative stress
The less accumulation of ROS and MDA in Dongdao-4 seedlings under salt stress prompted us to check the activities of the major antioxidant enzymes. Under control conditions, activities of SOD and CAT were comparable in shoots of Dongdao-4 and Jigeng-88 plants, while activities of POD were higher in shoots of Jigeng-88 than those in Dongdao-4 (Fig. 3d–f). There were marked increases in activities of these enzymes for rice seedlings upon exposure to salt stress, and the treatment-induced increases in activities of POD and SOD had no significant differences in shoots of Dongdao-4 and Jigeng-88 plants (Fig. 3e, f). In contrast, the magnitude of the treatment-induced increases in activities of CAT was greater in Dongdao-4 than in Jigeng-88 plants, such that a significantly higher content of CAT in Dongdao-4 than in Jigeng-88 was observed under salt stress (Fig. 3d). These results suggest that higher activities of CAT may contribute to greater tolerance of Dongdao-4 seedlings to salt stress by counteracting oxidative stress evoked by salt stress.
Summary of sequencing results and their matches in the Oryza sativa Japonica. cv. Nipponbare genome
Total mapped reads
Validation of differentially regulated genes (DEGs) by real-time PCR analysis
To validate the data from RNA-sequencing, 15 DEGs were randomly selected for real-time PCR analysis in both genotypes in response to salt stress. The primers of selected genes are listed in Additional file 2: Table S1. A high degree of concordance was observed between the results generated by the two methods (Pearson correlation coefficients R2 = 0.6843; Additional file 1: Figure S5).
Identification of DEGs of Dongdao-4 and Jigeng-88 plants under salt stress
Gene ontology (GO) categorization of DEGs
Dongdao-4 is an elite rice genotype that is capable of growing in saline-alkaline soils in the northeast of China. Our previous studies revealed that Dongdao-4 plants are equipped with an efficient system for acquisition iron under saline-alkaline conditions, thus conferring tolerance to saline-alkaline stress . In the present study, we evaluated the effect of moderate salt stress on Dongdao-4 plants and compared with that of a saline-alkaline-sensitive genotype, Jigeng-88. Our results demonstrated that, similar to saline-alkaline stress, Dongdao-4 plants were also more tolerant to salt stress than Jigeng-88 plants, as evidenced by less reduction in plant height and shoot biomass (Additional file 1: Figure S1). The greater performance of Dongdao-4 plants may be accounted for by their higher foliar chlorophyll contents and photosynthetic rates than those in Jigeng-88 plants under conditions of salt stress (Additional file 1: Figure S2). Moreover, there were no difference in K+ concentrations, Na+ concentrations and Na+/K+ ratio in shoots between Dongdao-4 and Jigeng-88 plants under salt stress (Additional file 1: Figure S3), suggesting that acquisition of Na+ and K+ may not account for the greater tolerance of Dongdao-4 than Jigeng-88 to salt stress. In addition to Na+ toxicity, plants suffering from salt stress also need to deal with osmotic stress. One important finding in the present study is that Dongdao-4 seedlings were capable of accumulating greater amount of soluble sugars, Pro than Jigeng-88 when exposed to moderate salt stress (Figs. 1 and 3). The greater accumulation of soluble sugars and Pro would confer Dongdao-4 plants more effective osmoregulation, thus we further compared transcript profiles of Dongdao-4 with Jigeng-88 in response to salt stress. Our results revealed that Dongdao-4 showed some specific biological processes may underlie the mechanism by which Dongdao-4 has greater tolerance than Jigeng-88. These findings highlight that effective osmoregulation by accumulation of soluble sugars and Pro to maintain water potential and up-regulation of ROS detoxifying systems underlie the greater tolerance of Dongdao-4 to salt stress.
Plants suffering from salt stress have to cope with high concentrations of Na+ in the growth medium. Foliar accumulation of Na+ in the cytosol may be toxic by inhibiting photosynthesis and other metabolic processes. K+ is the most abundant inorganic cation in plants, and plays a diverse role in many physiological processes. Therefore, maintaining an optimal K+/Na+ ratio is an important indicator of plant tolerance to saline stress. However, there are reports demonstrating independence of salt tolerance and foliar Na+ concentration among wheat genotypes [6, 20]. In addition, Pires et al. (2015) observed a high variability of physiological response to salt stress between rice genotypes with similar foliar Na+ concentration, suggested that other mechanisms may be responsible for salt tolerance . Almeida et al. (2014) also reported that the maintenance of growth in the presence of 100 mM NaCl can be independent of the exclusion or accumulation of Na+ among tomato varieties . In the present study, a comparable Na+ concentration and Na+/K+ ratio was observed in Dongdao-4 and Jigeng-88 when challenged by salt stress (Additional file 1: Figure S3c), suggesting that tolerance of Dongdao-4 plants to salt stress is not achieved by minimizing Na accumulation.
Roy et al. (2014) reported that Na+ exclusion may be more effective under conditions of higher salinity, whereas ‘osmotic tolerance’ may be more important in moderately saline conditions . In addition, the introgression of TmHKT1;5-A that encodes a Na+ transporter from Triticum monococcum L. into the durum wheat resulted in a significant improvement in grain yield when growing in saline soils [24, 25]. However, the yield of durum wheat with TmHKT1;5-A was similar to that cultivar without the introgressed gene, under low and moderate saline conditions [24, 25], suggesting that osmotic stress has a greater effect on yield of these plants growing in low to moderate saline soils than Na+ toxic effect . Therefore, our results highlight, that a higher osmoregulation capacity may confer greater tolerance of Dongdao-4 to moderate, neutral salt stress (i.e., 60 mM NaCl).
Accumulation of compatible solutes (e.g. Pro, raffinose, and glycine betaine) is a common phenomenon for plants to adapt to salt stress by maintaining turgor . In addition, the compatible solutes can also be used to stabilize proteins and cellular structures, and to counteract oxidative stress associated with abiotic stress. In the present study, we found that Dongdao-4 plants accumulated greater amount of soluble sugars and Pro than Jigeng-88 when exposed to salt stress (Fig. 5). We further demonstrated that the expression levels of genes encoding putative Pro synthase and transporters in Dongdao-4 were higher than those in Jigeng-88 under salt stress (Fig. 5), suggesting that the higher expression levels of these genes may underpin the greater accumulation of Pro in Dongdao-4 plants, thus contributing to their greater tolerance to salt stress. The higher Pro and soluble sugar concentration in Dongdao-4 may confer Dongdao-4 plants more effectively osmo regulation, thus accounting for the greater tolerance to osmotic stress than Jigeng-88 (Table 1).
Reactive oxygen species (ROS), as byproducts of photosynthesis, respiration, photorespiration have to maintain in an optimal level to protect plants from oxidative stress and to function as signaling molecules mediating many physiological processes in plants [26, 27]. To avoid excessive accumulation of ROS under salt stress, ROS scavenging mechanisms including enzymatic and non-enzymatic (antioxidants) are often activated in plants. Recent studies reported that an increase in activity of antioxidative enzymes enhanced salt tolerance in rice [11, 28, 29], wheat [30, 31], cotton , barley , alfalfa . Overexpressing genes encoding antioxidant enzymes enhanced salt tolerance in transgenic plants [12, 13, 35]. In present study, we found that salt stress-induced increases in activities of CAT were greater in Dongdao-4 than in Jigeng-88 plants (Fig. 6), which may allow Dongdao-4 plant to more efficiently regulate ROS, thus conferring it more tolerance to salt stress. No significant differences in the expression level of genes encoding CAT between Dongdao-4 and Jigeng-88 seedlings were found, suggesting that epigenetic and post-transcriptional regulation may underlies the activity of CAT.
To further explore the mechanism underlying the greater tolerance of Dongdao-4 at transcriptome level, we treated Dongdao-4 and Jigeng-88 seedlings with 60 mM NaCl for one week, and analyzed their transcriptome. A total of 110 genes were specifically up-regulated in Dongdao-4 (Additional file 2: Tables S2 and S10). Sun et al. (2013) reported that overexpresion of GsSRK in Arabidopsis enhanced salt tolerance and higher yields under salt stress . In this study, one gene encoding G-type lectin S-receptor-like serine/threonine-protein kinase (GsSRK) was up-regulated (Additional file 2: Table S2). As shown in Additional file 1: Figure S6a, the magnitude of the increase was greater in Jigeng-88 than in Dongdao-4, leading to a signifcantly higher expression levels in Dongdao-4. In addition, nine genes encoding lectin receptor-like kinases that play crucial roles in stress perception , were found to be up-regulated. Thaumatin-like proteins (TLPs) is involved in abiotic stresses including salinity and drought [38–42]. Here, one gene encoding thaumatin-like protein was specifically up-regulated by 2.6 fold in Dongdao-4 (Additional file 2: Table S2). Furthermore, WRKY transcription factor 6 and 46 were specifically up-regulated in Dongdao-4 respectively (Additional file 2: Table S2). Validation of OsWRKY46 by Real-time PCR suggested that the expression level of OsWRKY46 was up-regulated in both genotypes by salt stress, the increase was much greater in Dongdao-4 than in Jigeng-88 (Additional file 1: Figure S6b). OsWRKY46 is invovled in regulation of a set of genes associated with cellular osmoprotection and oxidative detoxification under drought and salt stress . To determine if a common transcription factors underlie the specifically up-regulation of genes in Dongdao-4, promoter of these genes were analysed by PlantCARE. As shown in the Additional file 2: Table S10, many motifs have been found in the promoters of these genes by promoter element analysis, such as ABRE (cis-acting element involved in the abscisic acid responsiveness), W-box (WRKY binding site), CGTCA-motif (cis-acting regulatory element involved in the MeJA-responsiveness), MBS (MYB binding site involved in drought-inducibility). However, there are no motif shared by all these genes. Therefore, we speculated that these specifically up-regulated genes may be regulated by multiple transcriptional factors rather than a common transcriptional factor.
A total of 107 genes were up-regulated in both Dongdao-4 and Jigeng-88 (Fig. 4). There are 3 genes with the magnitude of induction being higher in Dongdao-4 than in Jigeng-88, including Os08g0518900, Os12g0564100 and Os03g0847800. Os08g0518900 encoding xylanase inhibitor protein 1 is involved in defence responses that mainly due to biotic stress. Dimkpa et al. (2009) reported that defence response to biotic stress can alleviate abiotic stress conditions, including drought and salinity . These cross-tolerance between abiotic and biotic stress may induce a positive effect in enhancing abiotic tolerance in plants [45–47]. Therefore, the higher transcription levels of genes involved in defence response may contribute to greater tolerance of Dongdao-4 seedlings. MYB transcription factors were reported to be involved in various abiotic stress in plants . Os12g0564100, encoding a MYB transcription factor, may also underly the mechanism of greater tolerance to salt stress in Dongdao-4.
Among the commonly suppressed genes in both genotypes, nine genes were inhibited greater in Dongdao-4 than in Jigeng-88 by salt treatment. These genes encoding myo-inositol oxygenase, mannose-1-phosphate guanylyltransferase and protein involving in photosynthesis, are reported to be involved in plant adaption to abiotic stresses. Myo-inositol oxygenase which catalyzes the oxidation of free myo-inositol to D-glucuronate, are related to osmotic balance and possibly to the transport of Na+ from root to shoot in the common ice plant Mesembryanthemum crystallinum . Cotsaftis et al. (2011) also found that transcript levels of genes encoding myo-inositol oxygenase was significantly down-regulated in salt-tolerant lines FL478 and Pokkli . In addition, Das-Chatterjee et al. (2006) also reported that increased production of myo-inositol through overexpression of the L-myo-inositol 1-phosphate synthase gene in transgenic rice provided the plants with increased salinity tolerance . In our results, one gene encoding myo-inositol oxygenase was significantly down-regulated in both genotypes, while the magnitude of reduction in Dongdao-4 was significantly higher than Jigeng-88 (Additional file 1: Figure S6c, Additional file 2: Table S7). This results indicated that decrease of the transcript levels of genes encoding enzymes that degrade or synthesize compatible solutes will be a mechanism to reduce the effects of the imposed stress, thus conferring greater tolerance of Dongdao-4 plants to salt stress.
In conclusion, we report here that Dongdao-4 was more tolerant to salt stress than Jigeng-88 in spite of an similar Na+ concentration in shoot of both genotypes under salt stress. The greater salt-tolerant in Dongdao-4 may be caused by their greater capapcity to synthesize and accumulation of soluble sugars and Pro and higher activities of CAT under salt stress. Our findings demonstrate that the greater tolerance of Dongdao-4 to osmotic stress and efficient ROS detoxifying system underpin the higher tolerance to moderate salt stress than that of Jigene-88. The information about RNA-seq dataset also provides us potent and valuable guidance in underlying the mechanism with which Dongdao-4 has greater salt-tolerance than Jigeng-88.
Plant materials, growth conditions, and stress treatments
Two rice genotypes, Oryza sativa L. ssp. Japonica (cv. Dongdao-4 and Jigeng-88), were used in this study. Seeds were germinated in tap water at 37 °C for 2 days and transferred on moist tissue paper for 2 days at 30 °C in the dark. Thereafter the germinated seedlings were transferred to nutrient solution containing (mM): 1.425 NH4NO3, 0.42 NaH2PO4, 0.510 K2SO4, 0.998 CaCl2, 1.643 MgSO4, 0.168 Na2SiO3, 0.100 Fe-EDTA, 0.019 H3BO3, 0.009 MnCl2, 0.155 CuSO4, 0.152 ZnSO4, and 0.075 Na2MoO4. This hydroponic experiment was carried out in a growth chamber with 30 °C/22 °C (day/night) with 14-h photoperiod, and the relative humidity was controlled at approximately 70%.
For analysis of tolerance to salt stress, Dongdao-4 and Jigeng-88 plants were grown in the culture solution for 3 weeks. Then, half of plants were transferred to culture solution supplemented with 20 mM NaCl for one day, and 40 mM NaCl for one day, and then exposed to 60 mM NaCl for one week. The remaining half of plants was kept in the medium without NaCl as controls. Medium pH was adjusted to 5.8 and the solution was renewed every 3 d for both control and NaCl-supplemented media. To evaluate the effects of osmotic stress on the two rice genotypes, Dongdao-4 and Jigeng-88 seedlings were cultured in the control hydroponic solution for three weeks, and then transferred to the hydroponic solution containing 40 mM mannitol for 1 day, and 80 mM mannitol for 1 day, and then exposed to 120 mM mannitol for 5 days. After treatment, height, shoot biomass, and root biomass were measured. Shoots and roots of the rice seedlings were harvested and oven-dried at 75 °C for 2 days until their weight reached constant for determination of dry weight.
Determination of Na+ and K+ concentration
Shoots and roots of rice seedlings were harvested and dried as described in the above section. They were digested with the 6 mL of nitric acid and 2 mL of perhydrol with microwave system (MARS, CEM) after grinding into fine powder. Total ion contents were determined using inductively coupled plasma mass spectrometry (ICAP6300; Thermo Scientific, Waltham, MA).
Measurements of total root length
To analyze total root length, roots were scanned with an Epson digital scanner (Expression 10000XL, Epson Inc.) and analyzed with the WinRHIZO/WinFOLIA software (Regent Instruments Inc.).
Measurements of chlorophyll (CHL) concentration
Newly formed leaves were harvested, weighed, and extracted with aqueous ethanol (95% v/v) in both Dongdao-4 and Jigeng-88 plants, in order to determine CHL concentration. Absorbance (A) readings of the supernatant was recorded at wavelengths of 663 and 645 nm. Total CHL concentration was calculated as 8.02A663 + 20.21A645, and was expressed as mg chlorophyll g−1 fresh weight.
Measurements of photosynthetic characteristics
Photosynthetic rates of rice seedlings were measured between 8:30–11:30 with a LI-6400 XT portable photosynthesis system equipped with a LED leaf cuvette (Li-Cor, 146 Lincoln, NE, USA). Artificial illumination was applied to the leaves in the chamber from a red-blue 6400-02B LED light source attached to the sensor head with continuous light (1000 μmol m−2 s−1 photosynthetic photon flux density) and ambient CO2 concentration of approximately 500 μmol CO2 per mol. At least 15 individual Dongdao-4 and Jigeng-88 plants in each stress treatment were selected for measuring photosynthetic rates.
Determination of Pro and soluble sugars
Approx. 100 mg dried shoot-materials were extracted in 5 mL 80% ethanol at 80 °C for 1 h, then cooled at room temperature. Approx. 50 mg active carbon were added to the extract for 30 min and then centrifuged at 6000 g for 10 min. The supernatants were filtered and used to determine concentrations of Pro and soluble sugars. Pro contents in rice leaves were determined by the method described previously [48, 52]. In brief, 2 mL filtrate was incubated with 2 mL ninhydrin reagent (2.5% (w/v) ninhydrin, 60% (v/v) glacial acetic acid, 40% 6 M phosphoric acid) and 2 mL of glacial acetic acid at 100 °C for 1 h, and the reaction were terminated in an ice bath. 4 mL toluene was added into the mixtures, followed by vibrating and incubation at room temperature. The absorbance was measured at wavelength of 520 nm using a spectrophotometer (SmartSpecTM Plus, BioRad).
Total soluble sugar content was measured following the methods used previously [48, 52]. Briefly, 5 mL anthrone reagent was added to 1 mL extract incubating at 95 °C for 15 min, and then cooled at room temperature. The absorbance was measured at wavelength of 625 nm using a spectrophotometer (SmartSpecTM Plus, BioRad).
Visualization of reactive oxygen species (ROS)
The formation of hydrogen peroxide and superoxide anion radicals was detected by 3,3′-diaminobenzidine (DAB) staining and nitroblue tetrazolium (NBT) staining, respectively, as described previously by Wohlgemuth et al. (2002) . In brief, rice leaves from triplicate biological replicates of the samples were first cut into sections (c. 1 cm in length) and then immersed in 40 mL staining solution (0.1% (w/v) DAB, pH 6.5, or 0.1% (w/v) NBT, 10 mM sodium azide, 50 mM potassium phosphate, pH 6.4) in a desiccator. Infiltration was carried out by building up a vacuum (∼100–150 mbar) and until the leaves were completely infiltrated. The incubation was conducted in a growth chamber in the dark overnight.
Determination of malondialdehyde (MDA)
Malondialdehyde (MDA) content in rice leaves was determined following the protocols described by Song et al. (2011) . Briefly, rice leaves were weighed and homogenized in 5 mL of 10% TCA solution, and then centrifuged at 10,000 g for 10 min. Thereafter 2 mL supernatant was added in 2 mL of 10% trichloroacetic acid containing 0.6% thiobarbituric acid. The mixture was then incubated in water at 95 °C for 30 min and the reaction was stopped in an ice bath. The absorbance of the solution was measured at 450, 532, and 600 nm, respectively.
Determination of peroxidase, superoxide dismutase, and catalase activity
Approx. 0.5 g rice leaves were ground thoroughly with a cold mortar and pestle in 50 mM potassium phosphate buffer (pH 7.8) containing 1% polyvinylpyrrolidone. The homogenate was centrifuged at 15,000 g for 20 min at 4 °C. The supernatant was crude enzyme extraction. The activities of peroxidase (POD; EC 22.214.171.124), superoxide dismutase (SOD; EC 126.96.36.199), and catalase (CAT; EC 188.8.131.52) were measured using the protocols described by Yang et al. (2012) .
RNA isolation and real-time RT-PCR
Total RNA of shoots and roots was isolated using RNAiso reagent (Takara) and reverse-transcribed into first-strand cDNA with a PrimeScript® RT Reagent kit (Takara). Real-time PCR was performed in an optical 96-well plate with an Applied Biosystems Stepone™ Real-Time PCR system. Each reaction contained 7.5 μL of 2× SYBR Green Master Mix reagent, 0.5 μL of cDNA samples, and 0.6 μL of 10 μM gene-specific primers in a final volume of 15 μL. The thermal cycle used was as follows: 95 °C for 10 min, and 40 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30s. All the primers used for quantitative RT-PCR are listed in Additional file 2: Table S1. The relative expression level was analyzed by the comparative Ct method.
Total RNA extraction, cDNA library construct ion and Illumina deep sequencing
Total RNA was extracted as described above. The preparation of whole transcriptome libraries and deep sequencing were performed by the Annoroad Gene Technology Corporation (Beijing, PR China). Whole transcriptome libraries were constructed using TruSeq Stranded Total RNA with Ribo-Zero Gold (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system and qPCR (Kapa Biosystems, Woburn, MA, USA). The resulting libraries were sequenced initially on a HiSeq 2000 instrument that generated paired-end reads of 100 nucleotides.
Sequencing data analysis
The original image data were transferred into sequence data by base calling, which are defined as raw reads and saved as FastQ files. Prior to data analysis, the raw reads were filtered to obtain the clean reads by removing adaptors, tags of reads and low-quality reads. Then, the remaining high-quality reads were submitted for mapping analysis against the reference genome sequences (ftp://ftp.ensemblgenomes.org/pub /plants/release-30, last accessed November 30, 2015) using Tophat . Differentially expressed genes (DEGs) were identified using the empirical criterion of a greater than 2-fold change and a significant q value (false discovery rate-adjusted P value) of <0.05 based on two independent biological replicates. GO enrichment was performed using Cytoscape software (http://www.cytoscape.org, version 2.5.2) with Bingo plugin (http://www.psb.ugent.be/cbd/papers/BiNGO/, version 2.3). Hypergeometric test with Benjamini & Hochberg false discovery rate (FDR) were performed using the default parameters to adjust the P-value.
All data were analyzed by the analysis of variance using the SAS statistical software. Significant differences were evaluated using student’s t-test.
This study was supported by the National Natural Science Foundation of China (31301832) and Chinese Academy of Sciences (XDA08010401).
The National Natural Science Foundation of China and Chinese Academy of Sciences.
Availability of data and materials
All data generated or analysed during this study are included in this published article and its supplementary information files.
QL, AY and WHZ designed the experiments; QL and AY conducted the experiments; QL and AY analyzed the data; QL and AY WHZ wrote the paper. All authors read and approved the final manuscript.
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