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Fig. 7 | BMC Plant Biology

Fig. 7

From: microRNAs participate in gene expression regulation and phytohormone cross-talk in barley embryo during seed development and germination

Fig. 7

miR393-mediated auxin signalling regulation affects grain phenotypes and phytohormone cross-talk during seed germination. a: Seed phenotype of wildtype, 35S::MIR393b and 35S::MIM393 transgenic barley; b: Length-width ratio of seeds in wildtype, 35S::MIR393b and 35S::MIM393 transgenic barley; c: 1000-grain weight of wildtype, 35S::MIR393b and 35S::MIM393 transgenic barley; Asterisks indicate significant difference compared with wildtype. *, P < 0.05 (Student’s t-test); d: Relative expression levels of auxin-responsive genes in wildtype, 35S::MIR393b and 35S::MIM393 transgenic barley. Total RNA was isolated from 3 d-old seedlings. The expression level in wildtype was set as 1.0. Error bars represent the SD from three independent experiments; e-f: Relative expression level of ABA and GA-related genes in wildtype, 35S::MIR393b and 35S::MIM393 transgenic barley. Total RNA was isolated from embryo and cotyledon (e) and root (f) in 3 d-old seedlings. The expression level in wildtype was set as 1.0. Error bars represent the SD from three independent experiments. Different letters represent significantly different values at P < 0.05 (Duncan’s multiple range test)

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