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Fig. 5 | BMC Plant Biology

Fig. 5

From: The lysin motif-containing proteins, Lyp1, Lyk7 and LysMe3, play important roles in chitin perception and defense against Verticillium dahliae in cotton

Fig. 5

Silencing of Lyp1 and Lyk7 significantly impaired the resistance to V. dahliae in G. barbadense cv. Hai7124. Lyp1 and Lyk7 were silenced in V. dahliae resistant Hai 7124 seedlings by VIGS, and about 2 weeks later, the seedlings were inoculated with V. dahliae at a concentration of 1 × 107 spores/mL. a Analysis of Lyp1 and Lyk7 expression levels. Total RNA was extracted from the leaves of the VIGS seedlings 14 d post-agroinfiltration, and transcription levels of Lyp1 and Lyk7 in the Lyp1 and Lyk7-silenced plants were compared with that of the control (TRV: 00 plants). Asterisks indicate statistically significant differences, as determined by Student’s t-tests (**P < 0.01). b Disease symptoms of the Lyp1 and Lyk7-silenced plants 20 and 25 days after V. dahliae inoculation; c The percentage of diseased leaves of the Lyp1 and Lyk7-silenced plants and controls after V. dahliae inoculation. These experiments were repeated using at least 20 seedlings per treatment. Error bars show the standard deviation of three biological replicates. Asterisks indicate statistically significant differences in the percentage of diseased leaves between treated plants and TRV: 00 controls, as determined by Student’s t-tests (*P < 0.05, **P < 0.01)

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