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Fig. 3 | BMC Plant Biology

Fig. 3

From: Constitutive expression of OsDof4, encoding a C2-C2 zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)

Fig. 3

Transcriptional activity assay of OsDof4. a Schematic representation of effectors, reporter, and internal control plasmids structure. b The transcriptional activity of each effector. GAL4DBD and GAL4DBD-VP16 represented respectively negative and positive control effector. The plasmids of effector, reporter, and internal control were co-transformed into rice protoplasts, after overnight incubation in dark at 28 °C, the relative LUC activity of each effector was measured. With the relative LUC activity of GAL4DBD normalized to 1, obviously GAL4DBD-VP16 and GAL4DBD-OsDof4 showed higher transcriptional activity. Values are means ± SE (n = 5). Error bar indicates SE. The double asterisks indicate significant difference (p < 0.01) compared to GAL4DBD value according to student’s t test

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