Table 1 Primer sequences used in this study
Primer name | Primer sequence (5′-3′) | Position on scaffold sequence | Annealing temperature (°C) | PCR product size | Function |
|---|---|---|---|---|---|
Flo2-1F | TGTGCTGGAATCACCCACTC | 793–812 | 60 | 1061 | cloning TaFlo2 /polymorphism detection |
Flo2-1R | GCGCGGCGAAAACTAATCAT | 1853–1844 | |||
Flo2-2F | GTGCCGTCCATAATCGTTGC | 1546–1565 | 60 | 1781 | cloning TaFlo2 /polymorphism detection |
Flo2-2R | CATGTGCGGCAAAAGACACA | 3326–3307 | |||
Flo2-3F | AACGGGCATGTGTCTTTTGC | 3299–3318 | 60 | 3025 | cloning TaFlo2 /polymorphism detection |
Flo2-3R | CGACGCAGCTCTGAAAATCG | 6332–6313 | |||
Flo2-4F | CGCTTAGCAGTGGATTTGCC | 5719–5738 | 60 | 3948 | cloning TaFlo2 |
Flo2-4R | ATCCAACAAACAGGTGCCCA | 9667–9647 | |||
Flo2-5F | TTGCGGAAGCCCATCATTCT | 8387–8406 | 60 | 3836 | cloning TaFlo2 |
Flo2-5R | TGACCTTCTGCGGATGCTTT | 1222–12,203 | |||
Flo2-6F | CAGAACAGGGCCGGTACAAT | 11,368–11,387 | 60 | 2600 | cloning TaFlo2 |
Flo2-6R | CGCTCATCTGGATAGGGCAA | 13,967–13,948 |  | ||
TaFlo2-InDel8F | ACCCCTCCTCCGTTATCGTC | 1337–1356 | 60 | 145/153 | 8-bp InDel polymorphism in TaFlo2-A1 |
TaFlo2-InDel8R | CCTCCTTCTTCTTGCGGTCG | 1470–1489 | |||
Flo2-A1F | GTGCTCCGATCCGATGTGCAGTTAT | 5387–5411 | 58 | 587 | 2A specific |
Flo2-A1R | GTGCACAACCAAGTAAAAGG | 5973–5954 | |||
Flo2-B1F | GTC ATC ACTAGAGGA ATTTTCC | 6851–6872 | 58 | 902 | 2B specific |
Flo2-B1R | CTCTCAGAACTGTGGAT | 7752–7736 | |||
Flo2-D1F | CTGTATCTGTAATTTGTTCCG | 5378–5398 | 58 | 326 | 2D specific |
Flo2-D1R | CTTCCGAAAAATGTGGGG | 5704–5687 | |||
mFlo2-1F | TAACGGTGGTGCACTTGTGT | – | 58 | 1868 | Cloning mRNA |
mFlo2-1R | TCAGCCGCAAGTTATGCTCA | – | |||
mFlo2-2F | TGCGGACGAGATGGAAAACA | – | 58 | 1809 | Cloning mRNA |
mFlo2-2R | AGCAGTCAGCCGATGGTATG | – | |||
mFlo2-3F | ATGCGTACTCCCTAAGCGTG | – | 58 | 1889 | Cloning mRNA |
mFlo2-3R | CACGAAGTGCTGCTTGCTTT | – | |||
eTaFlo2F | CCATTCGGCTTTCGTGCAAA | – | 55 | 134 | Expression analysis |
eTaFlo2R | TGTTTTCCATCTCGTCCGCA | – | |||
ActinF | AGCCATACTGTGCCAATC | – | 55 | 134 | Internal control |
ActinR | GCAGTGGTGGTGAAGGAGTAA | – |