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Table 2 Insertional polymorphism levels of 6 MITEs in wild emmer wheat populations as observed in TD

From: Transposable elements generate population-specific insertional patterns and allelic variation in genes of wild emmer wheat (Triticum turgidum ssp. dicoccoides)

TEa family

 

Populationb

Totalc

  

Mt.Hermon

Amiad

Tabgha

Jaba

Mt.Amasa

 

Aison

Polymorphic bands

50

62

57

45

25

92

Total number of bands

72

78

72

70

60

99

Polymorphism level (%)

69.4

79.5

79.2

64.3

41.7

91.9

Eos

Polymorphic bands

37

39

50

26

23

69

Total number of bands

75

75

79

66

68

92

Polymorphism level (%)

49.3

52.0

63.3

39.4

33.8

75.0

Fortuna

Polymorphic bands

52

51

58

54

41

61

Total number of bands

65

65

68

68

54

70

Polymorphism level (%)

80.0

78.5

85.3

79.4

75.9

87.1

Minos

Polymorphic bands

18

23

13

15

9

34

Total number of bands

81

83

76

80

79

88

Polymorphism level (%)

22.2

27.7

17.1

18.8

11.4

38.6

Oleus

Polymorphic bands

36

28

27

38

20

48

Total number of bands

81

80

79

85

76

92

Polymorphism level (%)

44.4

35.0

34.2

44.7

26.3

52.2

Tantalos

Polymorphic bands

56

48

63

70

43

96

Total number of bands

100

95

99

107

92

116

Polymorphism level (%)

56.0

50.5

85.9

65.4

46.7

82.8

  1. aTD reactions were performed for 6 MITE families using: (1) two primer combinations, Aison-specific primer/M-CTA and Aison-specific primer/ M-CTC; (2) two primer combinations, Eos-specific primer/M-CTA and Eos-specific primer/M-CTG; (3) Fortuna-specific primer/M-CTA; (4) Minos-specific primer/M-CTA and Minos-specific primer/M-CTG; (5) Oleus-specific primer/M-CTA and Oleus-specific primer/M-CTG; (6) Tantalos-specific primer/M-CTA and Tantalos-specific primer/M-CTG. See Additional file 1: Table S2 for details on primer sequences
  2. bTD band was considered polymorphic when it was present/or absent in at least one accession. Total number of bands is the sum of polymorphic and monomorphic bands
  3. cBased on the analysis of all 50 accessions together, indicating the overall level of polymorphism in the collection set