Skip to main content
Fig. 1 | BMC Plant Biology

Fig. 1

From: Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Fig. 1

Schematic representation of sample preparation procedure by ChF, PF, and HPF from pollen germination to resin embedding. Arabidopsis PT preparation for ultrastructural studies showing the germination setup in the glass bottom petri dish, the germinated PTs that have grown through the stigma into the A-PGM, and the fixation, dehydration/freeze substitution, infiltration, and embedding steps. During embedding, individual specimens are carefully held with tweezers by the stigma of the pistil and slowly pulled along in the embedding resin to straighten out and align the PTs. A Teflon coated glass slide bearing a sample label is gently placed on top of the samples on the Teflon embedding mould (white block)

Back to article page